MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1680 986.4759 985.4686
1681 986.4763 985.4690
1682 987.4611 986.4538
1683 987.4666 986.4593
1684 987.4741 986.4668
1686 494.7267 987.4388
1687 988.4795 987.4722
1688 988.4795 987.4722
1689 989.4678 988.4605
1690 989.4700 988.4627
1691 989.4989 988.4916
1692 989.9758 988.9685
1693 330.8125 989.4157
1694 990.4832 989.4759
1695 991.4795 990.4722
1697 991.4833 990.4760
1698 991.5245 990.5172
1700 992.4768 991.4695
1701 992.4789 991.4716
1702 496.7440 991.4734
1703 496.7447 991.4748
1704 496.7623 991.5100
1705 496.7633 991.5120
1706 496.7650 991.5154
1707 496.7652 991.5158
1708 496.7655 991.5164
1709 496.7663 991.5180
1710 993.4673 992.4600
1711 993.4753 992.4680
1712 993.4885 992.4812
1713 994.4772 993.4699
1714 996.4608 995.4535
1715 996.4728 995.4655
1719 996.4818 995.4745
1720 996.4949 995.4876
1721 997.4589 996.4516
1724 499.2339 996.4532
1725 997.4777 996.4704
1726 997.4880 996.4807
1727 997.5176 996.5103
1728 998.4675 997.4602
1730 500.2418 998.4690
1731 999.4792 998.4719
1732 999.4879 998.4806
1734 1000.4846 999.4773
1735 1001.4823 1000.4750
1736 1003.4600 1002.4527
1737 1003.4722 1002.4649
1738 1003.4841 1002.4768
1739 1003.4844 1002.4771
1740 1003.4850 1002.4777
1741 335.4755 1003.4047
1742 1004.4742 1003.4669
1743 1004.4896 1003.4823
1744 1005.4751 1004.4678
1745 1005.4826 1004.4753
1747 1006.4777 1005.4704
1748 1006.4838 1005.4765
1749 503.7521 1005.4896
1751 1007.4686 1006.4613
1752 1007.4743 1006.4670
1753 1007.4849 1006.4776
1755 504.2626 1006.5106
1756 504.2628 1006.5110
1757 1007.5185 1006.5112
1758 1007.5193 1006.5120
1759 504.7260 1007.4374
1760 1008.4864 1007.4791
1761 504.7471 1007.4796
1762 1008.4913 1007.4840
1763 1008.4957 1007.4884
1764 1008.4960 1007.4887
1765 336.8460 1007.5162
1767 1009.4776 1008.4703
1768 1009.4819 1008.4746
1769 1009.4869 1008.4796
1770 1009.4885 1008.4812
1771 1010.4584 1009.4511
1772 1011.4987 1010.4914
1773 1012.4603 1011.4530
1774 506.7346 1011.4546
1775 506.7395 1011.4644
1776 1012.4820 1011.4747
1777 1012.4825 1011.4752
1778 1013.4901 1012.4828
1779 1013.4974 1012.4901
1780 1013.5056 1012.4983
1781 1014.4769 1013.4696
1782 1015.5005 1014.4932
1784 1016.4863 1015.4790
1785 1016.4935 1015.4862
1787 1017.4963 1016.4890
1788 1018.4905 1017.4832
1789 1018.4922 1017.4849
1790 1018.4935 1017.4862
1791 510.2239 1018.4332
1792 1019.4858 1018.4785
1793 1019.4869 1018.4796
1794 1020.4824 1019.4751
1795 1020.4844 1019.4771
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