MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1557 948.4584 947.4511
1559 949.4483 948.4410
1560 949.4565 948.4492
1562 949.5112 948.5039
1565 950.4429 949.4356
1566 950.4598 949.4525
1568 475.7388 949.4630
1570 950.4766 949.4693
1572 476.2159 950.4172
1573 476.2165 950.4184
1574 952.4526 951.4453
1575 952.4549 951.4476
1576 952.4576 951.4503
1577 952.5221 951.5148
1579 953.4528 952.4455
1580 953.4647 952.4574
1581 953.4647 952.4574
1582 954.4539 953.4466
1583 954.4710 953.4637
1586 955.4962 954.4889
1588 956.4312 955.4239
1589 478.7356 955.4566
1590 956.4662 955.4589
1592 957.4608 956.4535
1593 957.4621 956.4548
1594 958.4952 957.4879
1595 959.4607 958.4534
1596 959.4618 958.4545
1597 959.4634 958.4561
1598 959.4716 958.4643
1599 960.4515 959.4442
1600 960.4647 959.4574
1601 960.4736 959.4663
1602 480.7675 959.5204
1603 961.4336 960.4263
1604 961.4598 960.4525
1605 961.4849 960.4776
1606 962.4595 961.4522
1607 963.4679 962.4606
1608 964.4667 963.4594
1609 964.4700 963.4627
1610 964.4768 963.4695
1611 965.4600 964.4527
1612 483.2571 964.4996
1613 483.2578 964.5010
1614 966.4572 965.4499
1619 966.4655 965.4582
1620 483.7364 965.4582
1621 966.4683 965.4610
1622 483.7749 965.5352
1623 484.2135 966.4124
1624 967.4641 966.4568
1625 967.4697 966.4624
1626 967.4896 966.4823
1627 968.4534 967.4461
1628 968.4614 967.4541
1629 484.7355 967.4564
1630 968.4648 967.4575
1631 968.4650 967.4577
1633 968.5013 967.4940
1634 968.5117 967.5044
1635 969.4606 968.4533
1636 969.4646 968.4573
1637 969.4708 968.4635
1639 970.4578 969.4505
1640 970.4644 969.4571
1641 485.7456 969.4766
1643 971.4609 970.4536
1644 971.4661 970.4588
1645 972.4473 971.4400
1646 972.4635 971.4562
1647 973.4701 972.4628
1648 974.4577 973.4504
1649 974.4633 973.4560
1650 975.4578 974.4505
1651 975.4855 974.4782
1652 488.2691 974.5236
1653 976.4664 975.4591
1654 976.4675 975.4602
1655 976.4775 975.4702
1656 976.4817 975.4744
1657 488.7658 975.5170
1658 977.4622 976.4549
1659 977.4633 976.4560
1660 490.2288 978.4430
1661 979.4720 978.4647
1662 980.4623 979.4550
1663 980.4664 979.4591
1664 980.4727 979.4654
1666 981.4717 980.4644
1667 981.4734 980.4661
1668 981.4740 980.4667
1670 982.4539 981.4466
1672 983.4712 982.4639
1673 983.4766 982.4693
1674 984.4695 983.4622
1676 985.4721 984.4648
1677 985.4854 984.4781
1678 985.4868 984.4795
1679 986.4721 985.4648
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