MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1417 450.2520 898.4894
1419 900.4332 899.4259
1420 450.7256 899.4366
1421 902.4511 901.4438
1423 903.4316 902.4243
1424 904.4388 903.4315
1425 904.4496 903.4423
1426 905.4344 904.4271
1433 908.4617 907.4544
1434 909.4321 908.4248
1435 909.4353 908.4280
1436 909.4413 908.4340
1437 909.4504 908.4431
1438 910.4437 909.4364
1439 910.4440 909.4367
1442 911.4398 910.4325
1443 456.2321 910.4496
1448 456.2369 910.4592
1449 912.4306 911.4233
1450 913.4459 912.4386
1451 914.4377 913.4304
1454 915.4371 914.4298
1455 916.4362 915.4289
1457 916.4528 915.4455
1458 916.4541 915.4468
1459 917.4387 916.4314
1460 917.4401 916.4328
1461 918.4416 917.4343
1462 919.4304 918.4231
1463 919.4442 918.4369
1464 460.7154 919.4162
1465 920.4415 919.4342
1466 920.4438 919.4365
1468 921.4448 920.4375
1469 921.4483 920.4410
1470 921.4484 920.4411
1472 922.4310 921.4237
1474 462.7077 923.4008
1475 924.4082 923.4009
1477 925.4417 924.4344
1478 925.4956 924.4883
1480 926.4432 925.4359
1481 927.1998 926.1925
1482 927.4351 926.4278
1483 927.4481 926.4408
1484 927.4492 926.4419
1485 927.4501 926.4428
1486 309.8229 926.4469
1487 464.2314 926.4482
1488 928.4118 927.4045
1489 464.7416 927.4686
1490 464.7419 927.4692
1491 929.4443 928.4370
1492 929.4465 928.4392
1493 929.4586 928.4513
1494 930.4415 929.4342
1497 931.4382 930.4309
1498 931.4451 930.4378
1499 931.4538 930.4465
1500 931.4561 930.4488
1501 931.4571 930.4498
1502 932.4564 931.4491
1503 932.4577 931.4504
1504 466.7330 931.4514
1505 933.4509 932.4436
1507 467.7418 933.4690
1508 468.2183 934.4220
1509 935.4514 934.4441
1512 936.4551 935.4478
1513 936.4711 935.4638
1514 937.4380 936.4307
1515 937.4551 936.4478
1518 938.4509 937.4436
1519 938.4511 937.4438
1520 938.4563 937.4490
1521 939.4174 938.4101
1522 939.4431 938.4358
1523 939.4622 938.4549
1528 940.4496 939.4423
1529 940.4552 939.4479
1534 942.4523 941.4450
1535 942.4592 941.4519
1536 942.4621 941.4548
1537 942.4644 941.4571
1538 942.4665 941.4592
1539 942.4681 941.4608
1540 942.4740 941.4667
1542 943.4550 942.4477
1543 943.4635 942.4562
1544 944.4521 943.4448
1545 944.4581 943.4508
1546 944.4794 943.4721
1547 945.4487 944.4414
1548 945.4636 944.4563
1549 473.7353 945.4560
1551 947.4656 946.4583
1552 948.4408 947.4335
1553 948.4418 947.4345
1554 948.4484 947.4411
1556 948.4557 947.4484
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