MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 4 |
| MS data file | : | PRT1245_Other_P1_B12.mgf |
| Database | : | S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues) |
| Timestamp | : | 24 Jun 2025 at 21:22:18 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,772 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 2954 |
781.9064 | 1561.7982 | 1561.8239 | -16.4 | 0 | 8 | 0.17 | 1Score > 23 indicates identity Score > 13 indicates homology |
ATLSTLFQELNVPK + 2 Deamidated (NQ) |
| 1868 |
519.7709 | 1037.5272 | 1037.5366 | -9.03 | 1 | 8 | 0.34 | 1Score > 21 indicates identity Score > 16 indicates homology |
QNKVQHQR + Deamidated (NQ) |
| 1139 |
384.2067 | 766.3988 | 766.3861 | 16.6 | 0 | 8 | 0.57 | 1Score > 20 indicates identity Score > 18 indicates homology |
QLNYTK + Deamidated (NQ) |
| 2788 |
730.3585 | 1458.7024 | 1458.6965 | 4.05 | 1 | 8 | 1 | 1Score > 22 indicates identity Score > 21 indicates homology |
HNMAKIYAGFYK + Deamidated (NQ); Oxidation (M) |
| 3686 |
1028.4871 | 2054.9596 | 2054.9796 | -9.72 | 1 | 8 | 1 | 1Score > 22 indicates identity Score > 21 indicates homology |
LSDKEQQLEAELSDFFR + Deamidated (NQ) |
| 2943 |
520.2540 | 1557.7402 | 1557.7423 | -1.37 | 0 | 8 | 1 | 1Score > 23 indicates identity Score > 21 indicates homology |
IGDRPEFPNDLER + Deamidated (NQ) |
| 987 |
329.1824 | 656.3502 | 656.3605 | -15.7 | 0 | 8 | 0.36 | 1Score > 16 indicates identity |
LNAPSR |
| 900 |
584.3409 | 583.3336 | 583.3329 | 1.16 | 0 | 8 | 0.15 | 1Score > 13 indicates identity |
APATPK |
| 2128 |
551.2820 | 1100.5494 | 1100.5462 | 2.98 | 0 | 8 | 0.47 | 1Score > 21 indicates identity Score > 17 indicates homology |
IQAEGGAADIR + Deamidated (NQ) |
| 2282 |
571.2801 | 1140.5456 | 1140.5485 | -2.49 | 1 | 8 | 0.94 | 1Score > 20 indicates identity |
GIAEVNMKYT + Oxidation (M) |
| 2923 |
773.9026 | 1545.7906 | 1545.8012 | -6.80 | 1 | 8 | 1 | 1Score > 22 indicates identity Score > 20 indicates homology |
VASHKNSHNPSLVR + Deamidated (NQ) |
| 939 |
624.3356 | 623.3283 | 623.3279 | 0.71 | 0 | 8 | 0.98 | 1Score > 20 indicates identity |
VSGSFK |
| 2618 |
651.8721 | 1301.7296 | 1301.7455 | -12.2 | 1 | 8 | 0.83 | 1Score > 20 indicates identity |
SLDLYLAPRVR |
| 1896 |
523.7792 | 1045.5438 | 1045.5444 | -0.52 | 0 | 8 | 0.63 | 1Score > 23 indicates identity Score > 18 indicates homology |
LASWVIDNK + Deamidated (NQ) |
| 2027 |
540.2811 | 1078.5476 | 1078.5659 | -16.9 | 0 | 8 | 0.49 | 1Score > 23 indicates identity Score > 17 indicates homology |
STIIENFQK |
| 1185 |
398.2096 | 794.4046 | 794.4174 | -16.1 | 0 | 8 | 1 | 1Score > 21 indicates identity Score > 20 indicates homology |
VSTTYPK |
| 2305 |
573.2889 | 1144.5632 | 1144.5724 | -7.99 | 1 | 8 | 1 | 1Score > 21 indicates identity Score > 20 indicates homology |
SGQIANDPSKK + Deamidated (NQ) |
| 2850 |
751.4192 | 1500.8238 | 1500.8035 | 13.5 | 0 | 8 | 0.38 | 1Score > 20 indicates identity Score > 16 indicates homology |
NDTTLLEIVENLK |
| 984 |
657.3566 | 656.3493 | 656.3606 | -17.1 | 0 | 8 | 0.41 | 1Score > 16 indicates identity |
QPSIGR |
| 1222 |
406.2072 | 810.3998 | 810.4064 | -8.14 | 0 | 8 | 1 | 1Score > 21 indicates identity Score > 20 indicates homology |
WAPYFK |
| 2278 |
570.7698 | 1139.5250 | 1139.5281 | -2.67 | 0 | 8 | 1 | 1Score > 20 indicates identity Score > 20 indicates homology |
MAYTSHLSSK + Oxidation (M) |
| 1532 |
470.7642 | 939.5138 | 939.5066 | 7.74 | 0 | 8 | 0.73 | 1Score > 19 indicates identity |
FLGFDTIK |
| 929 |
308.6875 | 615.3604 | 615.3592 | 2.09 | 0 | 8 | 1 | 1Score > 25 indicates identity Score > 20 indicates homology |
AVIEGK |
| 1585 |
955.4621 | 954.4548 | 954.4593 | -4.66 | 1 | 8 | 0.85 | 1Score > 19 indicates identity |
MAAQKSFR + Deamidated (NQ); Oxidation (M) |
| 2634 |
660.3347 | 1318.6548 | 1318.6703 | -11.7 | 1 | 8 | 1 | 1Score > 23 indicates identity Score > 20 indicates homology |
RSNMFVPLPNK + Deamidated (NQ); Oxidation (M) |
| 960 |
641.3368 | 640.3295 | 640.3254 | 6.43 | 0 | 7 | 0.22 | 1Score > 20 indicates identity Score > 13 indicates homology |
YLAMK + Oxidation (M) |
| 2619 |
652.3010 | 1302.5874 | 1302.5728 | 11.2 | 0 | 7 | 0.61 | 1Score > 20 indicates identity Score > 18 indicates homology |
GQFGQTNYSTAK + 2 Deamidated (NQ) |
| 1096 |
372.2067 | 742.3988 | 742.3974 | 2.01 | 0 | 7 | 0.57 | 1Score > 18 indicates identity |
QGGSAPVK |
| 2394 |
583.2848 | 1164.5550 | 1164.5676 | -10.8 | 0 | 7 | 0.56 | 1Score > 23 indicates identity Score > 17 indicates homology |
NNLHAEGWPK |
| 1783 |
508.2656 | 1014.5166 | 1014.5094 | 7.15 | 0 | 7 | 0.95 | 1Score > 21 indicates identity Score > 20 indicates homology |
GLNNGENVAK |
| 1104 |
751.3752 | 750.3679 | 750.3773 | -12.5 | 0 | 7 | 0.51 | 1Score > 22 indicates identity Score > 17 indicates homology |
QNHPQK |
| 2182 |
557.2949 | 1112.5752 | 1112.5536 | 19.5 | 1 | 7 | 0.95 | 1Score > 20 indicates identity |
KCYTNVVTK + Deamidated (NQ) |
| 2757 |
723.8655 | 1445.7164 | 1445.7085 | 5.48 | 0 | 7 | 1 | 1Score > 22 indicates identity Score > 20 indicates homology |
SHGAVLPSYCSLR |
| 1094 |
372.2066 | 742.3986 | 742.4086 | -13.4 | 1 | 7 | 0.6 | 1Score > 18 indicates identity |
AGRTGPGK |
| 1123 |
380.2320 | 758.4494 | 758.4538 | -5.71 | 0 | 7 | 1 | 1Score > 22 indicates identity Score > 20 indicates homology |
VASLIEK |
| 2702 |
695.8745 | 1389.7344 | 1389.7252 | 6.66 | 1 | 7 | 0.22 | 1Score > 22 indicates identity Score > 13 indicates homology |
IRELLNFNDEK |
| 831 |
469.2775 | 468.2702 | 468.2696 | 1.28 | 0 | 7 | 0.19 | 1Score > 13 indicates identity |
GPAPK |
| 1390 |
446.7352 | 891.4558 | 891.4596 | -4.24 | 1 | 7 | 1 | 1Score > 23 indicates identity Score > 20 indicates homology |
LGMRGQSK + Oxidation (M) |
| 1526 |
470.2365 | 938.4584 | 938.4709 | -13.3 | 0 | 7 | 0.39 | 1Score > 20 indicates identity Score > 16 indicates homology |
NVTDYLSK |
| 1964 |
1062.4985 | 1061.4912 | 1061.4876 | 3.39 | 0 | 7 | 1 | 1Score > 21 indicates identity Score > 20 indicates homology |
NENLSNELK + 2 Deamidated (NQ) |
| 1733 |
1000.4792 | 999.4719 | 999.4556 | 16.3 | 0 | 7 | 0.76 | 1Score > 18 indicates identity |
NGGTDIHMR |
| 1039 |
348.6695 | 695.3244 | 695.3126 | 17.1 | 0 | 7 | 0.91 | 1Score > 19 indicates identity |
EEGYAK |
| 933 |
309.6665 | 617.3184 | 617.3133 | 8.40 | 1 | 7 | 0.77 | 1Score > 23 indicates identity Score > 18 indicates homology |
KNGNGK + Deamidated (NQ) |
| 1159 |
391.2021 | 780.3896 | 780.3991 | -12.1 | 0 | 7 | 0.63 | 1Score > 21 indicates identity Score > 18 indicates homology |
NIHQNR |
| 3939 |
722.3438 | 2164.0096 | 2163.9953 | 6.57 | 0 | 7 | 0.34 | 1Score > 22 indicates identity Score > 15 indicates homology |
NMTNSAASLATSPTSNQLNPK + 2 Deamidated (NQ); Oxidation (M) |
| 1188 |
398.7008 | 795.3870 | 795.3796 | 9.32 | 0 | 7 | 1 | 1Score > 20 indicates identity Score > 19 indicates homology |
MSNISTK + Oxidation (M) |
| 2062 |
544.2850 | 1086.5554 | 1086.5709 | -14.3 | 0 | 7 | 0.37 | 1Score > 22 indicates identity Score > 15 indicates homology |
EHTIIAEFK |
| 2547 |
617.2925 | 1232.5704 | 1232.5893 | -15.3 | 0 | 7 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
NQPGLSMMLNK + Deamidated (NQ) |
| 1079 |
368.1757 | 734.3368 | 734.3460 | -12.4 | 0 | 7 | 0.23 | 1Score > 20 indicates identity Score > 13 indicates homology |
NFQNGR |
| 2089 |
546.7903 | 1091.5660 | 1091.5546 | 10.5 | 0 | 7 | 0.27 | 1Score > 24 indicates identity Score > 14 indicates homology |
CVLGLFNNR |
| 2610 |
649.8043 | 1297.5940 | 1297.6184 | -18.7 | 1 | 7 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
MSSSVVGASSNKK + Deamidated (NQ); Oxidation (M) |
| 1665 |
980.4786 | 979.4713 | 979.4862 | -15.2 | 0 | 7 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
VTNFEEIK + Deamidated (NQ) |
| 2936 |
519.2555 | 1554.7447 | 1554.7249 | 12.7 | 1 | 7 | 0.49 | 1Score > 22 indicates identity Score > 16 indicates homology |
KGGVFNGSPFMNQR + Deamidated (NQ); Oxidation (M) |
| 1339 |
435.2662 | 868.5178 | 868.5243 | -7.38 | 1 | 7 | 0.37 | 1Score > 15 indicates identity |
ISLPQRR |
| 2964 |
785.3906 | 1568.7666 | 1568.7617 | 3.17 | 0 | 7 | 0.27 | 1Score > 21 indicates identity Score > 14 indicates homology |
MNNTSITGPQVLHR + 2 Deamidated (NQ) |
| 948 |
630.3303 | 629.3230 | 629.3133 | 15.5 | 0 | 7 | 0.43 | 1Score > 16 indicates identity |
TAQPGR + Deamidated (NQ) |
| 3557 |
991.4933 | 1980.9720 | 1980.9792 | -3.63 | 1 | 7 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
NLDTFLNQLRDFLNEK + 2 Deamidated (NQ) |
| 2572 |
630.3018 | 1258.5890 | 1258.5902 | -0.89 | 1 | 7 | 1 | 1Score > 20 indicates identity Score > 19 indicates homology |
QNLRQADDGNK + Deamidated (NQ) |
| 997 |
334.1928 | 666.3710 | 666.3813 | -15.4 | 0 | 7 | 0.57 | 1Score > 17 indicates identity |
GLPPQR |
| 1171 |
785.3797 | 784.3724 | 784.3576 | 18.9 | 0 | 7 | 0.87 | 1Score > 19 indicates identity |
GANGHSSR |
| 2181 |
557.2946 | 1112.5746 | 1112.5536 | 18.9 | 1 | 7 | 1 | 1Score > 20 indicates identity Score > 19 indicates homology |
KCYTNVVTK + Deamidated (NQ) |
| 1801 |
511.2730 | 1020.5314 | 1020.5200 | 11.2 | 1 | 7 | 0.31 | 1Score > 23 indicates identity Score > 14 indicates homology |
LTSGSGSERK |
| 1213 |
403.6985 | 805.3824 | 805.3752 | 8.98 | 0 | 7 | 0.93 | 1Score > 21 indicates identity Score > 19 indicates homology |
MANSLDR |
| 1403 |
898.3907 | 897.3834 | 897.3716 | 13.2 | 0 | 7 | 0.64 | 1Score > 17 indicates identity |
ADSGYEEK |
| 903 |
586.3201 | 585.3128 | 585.3235 | -18.2 | 0 | 7 | 0.92 | 1Score > 19 indicates identity |
AVSPGR |
| 1476 |
463.2228 | 924.4310 | 924.4487 | -19.1 | 0 | 6 | 1.1 | 1Score > 20 indicates identity |
QIMNTYR |
| 2067 |
544.2869 | 1086.5592 | 1086.5669 | -7.05 | 1 | 6 | 0.6 | 1Score > 22 indicates identity Score > 17 indicates homology |
ERQVELEGK |
| 884 |
555.3147 | 554.3074 | 554.3176 | -18.4 | 0 | 6 | 0.38 | 1Score > 15 indicates identity |
ITGHK |
| 1134 |
767.3695 | 766.3622 | 766.3497 | 16.3 | 0 | 6 | 1 | 1Score > 20 indicates identity Score > 19 indicates homology |
GDAETFK |
| 2922 |
773.9010 | 1545.7874 | 1545.7610 | 17.1 | 0 | 6 | 0.96 | 1Score > 22 indicates identity Score > 19 indicates homology |
QPCSPIIQHTHVK + 2 Deamidated (NQ) |
| 3829 |
704.0236 | 2109.0490 | 2109.0187 | 14.3 | 1 | 6 | 0.26 | 1Score > 23 indicates identity Score > 13 indicates homology |
MKDYSNIVEPTLEDPTLK + Deamidated (NQ); Oxidation (M) |
| 1921 |
1053.4884 | 1052.4811 | 1052.4635 | 16.7 | 0 | 6 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
NGGTSANYNR |
| 2966 |
785.8897 | 1569.7648 | 1569.7569 | 5.05 | 1 | 6 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
DIVASFMSRAGSASR + Oxidation (M) |
| 928 |
615.3464 | 614.3391 | 614.3388 | 0.57 | 0 | 6 | 0.99 | 1Score > 19 indicates identity |
GGLSPGK |
| 2465 |
1182.5618 | 1181.5545 | 1181.5537 | 0.66 | 0 | 6 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
ANGQVHATDNR |
| 3952 |
1086.5280 | 2171.0414 | 2171.0052 | 16.7 | 1 | 6 | 1 | 1Score > 23 indicates identity Score > 19 indicates homology |
LTYDMLSQDKIINNTNNR + 3 Deamidated (NQ); Oxidation (M) |
| 1669 |
491.7271 | 981.4396 | 981.4437 | -4.12 | 1 | 6 | 0.38 | 1Score > 21 indicates identity Score > 15 indicates homology |
QDTQKSMK + Deamidated (NQ); Oxidation (M) |
| 1083 |
370.7012 | 739.3878 | 739.3864 | 1.92 | 0 | 6 | 0.89 | 1Score > 18 indicates identity |
LHDINK + Deamidated (NQ) |
| 3106 |
834.3950 | 1666.7754 | 1666.7542 | 12.8 | 0 | 6 | 0.8 | 1Score > 23 indicates identity Score > 18 indicates homology |
INELSDLEMNGMIR + Deamidated (NQ); 2 Oxidation (M) |
| 927 |
613.2952 | 612.2879 | 612.2980 | -16.4 | 0 | 6 | 0.49 | 1Score > 16 indicates identity |
NHSGAK |
| 1943 |
528.7702 | 1055.5258 | 1055.5247 | 1.07 | 1 | 6 | 1 | 1Score > 20 indicates identity Score > 19 indicates homology |
EKGLTHEDK |
| 3398 |
931.9863 | 1861.9580 | 1861.9244 | 18.1 | 1 | 6 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
KNADNEPTLITFMVPR + Deamidated (NQ); Oxidation (M) |
| 2687 |
689.3376 | 1376.6606 | 1376.6684 | -5.64 | 1 | 6 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
ANRDIDFLQER + Deamidated (NQ) |
| 1510 |
468.2396 | 934.4646 | 934.4760 | -12.1 | 0 | 6 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
NPNTFTLK + Deamidated (NQ) |
| 2104 |
1096.5183 | 1095.5110 | 1095.5271 | -14.6 | 0 | 6 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
ISDFVCLDK |
| 3058 |
543.5939 | 1627.7599 | 1627.7802 | -12.5 | 1 | 6 | 0.28 | 1Score > 22 indicates identity Score > 13 indicates homology |
NEKGADVVATTNSHGK + Deamidated (NQ) |
| 3095 |
552.9404 | 1655.7994 | 1655.7791 | 12.2 | 1 | 6 | 1 | 1Score > 21 indicates identity Score > 19 indicates homology |
GLFKFSDVETQDGGR + Deamidated (NQ) |
| 926 |
612.3120 | 611.3047 | 611.3139 | -15.1 | 1 | 6 | 0.34 | 1Score > 14 indicates identity |
GRDHK |
| 2074 |
1088.5430 | 1087.5357 | 1087.5509 | -14.0 | 1 | 6 | 0.67 | 1Score > 22 indicates identity Score > 17 indicates homology |
QNVEKAAAEK + Deamidated (NQ) |
| 2831 |
745.8594 | 1489.7042 | 1489.6943 | 6.67 | 1 | 6 | 1 | 1Score > 22 indicates identity Score > 19 indicates homology |
NNVKPEDEMRSR + Oxidation (M) |
| 4009 |
739.7115 | 2216.1127 | 2216.0709 | 18.9 | 1 | 6 | 1 | 1Score > 22 indicates identity Score > 18 indicates homology |
SNHIDAFNKANSVEVNESLK + Deamidated (NQ) |
| 986 |
329.1823 | 656.3500 | 656.3606 | -16.0 | 0 | 6 | 0.61 | 1Score > 16 indicates identity |
QPSIGR |
| 2837 |
747.3969 | 1492.7792 | 1492.7522 | 18.2 | 1 | 6 | 1 | 1Score > 22 indicates identity Score > 18 indicates homology |
SPDLINKSTFQSR + Deamidated (NQ) |
| 1272 |
421.2266 | 840.4386 | 840.4341 | 5.40 | 0 | 6 | 1.2 | 1Score > 19 indicates identity |
NATPVNPK + Deamidated (NQ) |
| 1295 |
427.2304 | 852.4462 | 852.4527 | -7.62 | 0 | 6 | 1.2 | 1Score > 19 indicates identity |
FSIIMAR + Oxidation (M) |
| 1564 |
475.2601 | 948.5056 | 948.5029 | 2.93 | 1 | 6 | 0.52 | 1Score > 22 indicates identity Score > 16 indicates homology |
GPRTLYDK |
| 2457 |
1179.5735 | 1178.5662 | 1178.5601 | 5.19 | 1 | 6 | 1 | 1Score > 22 indicates identity Score > 18 indicates homology |
TSNNKQVMEK + Deamidated (NQ) |
| 1078 |
735.3308 | 734.3235 | 734.3381 | -19.9 | 0 | 6 | 1.2 | 1Score > 19 indicates identity |
QLDGMR + Oxidation (M) |
| 934 |
618.3268 | 617.3195 | 617.3207 | -1.87 | 0 | 6 | 0.73 | 1Score > 23 indicates identity Score > 17 indicates homology |
MGGLPK + Oxidation (M) |
| 1750 |
503.7705 | 1005.5264 | 1005.5165 | 9.93 | 1 | 6 | 1 | 1Score > 23 indicates identity Score > 18 indicates homology |
MKAGDDILK + Oxidation (M) |
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