MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1288 850.4046 849.3973
1289 850.4099 849.4026
1290 851.3904 850.3831
1291 852.4177 851.4104
1292 426.7200 851.4254
1293 853.4189 852.4116
1294 427.2299 852.4452
1297 853.4611 852.4538
1298 427.2491 852.4836
1300 427.7099 853.4052
1301 427.7101 853.4056
1302 854.4166 853.4093
1303 855.3856 854.3783
1305 855.4357 854.4284
1306 856.4120 855.4047
1308 857.3842 856.3769
1309 857.4030 856.3957
1310 857.4255 856.4182
1311 429.7125 857.4104
1314 858.4308 857.4235
1315 858.4326 857.4253
1316 429.7202 857.4258
1317 859.3793 858.3720
1319 859.4185 858.4112
1320 859.4318 858.4245
1322 861.4092 860.4019
1325 862.4119 861.4046
1326 862.4139 861.4066
1327 432.2407 862.4668
1328 865.4052 864.3979
1329 865.4178 864.4105
1330 434.1961 866.3776
1331 867.3851 866.3778
1332 867.3853 866.3780
1333 434.1965 866.3784
1335 867.4246 866.4173
1336 869.4201 868.4128
1337 869.4219 868.4146
1338 869.4319 868.4246
1340 871.4124 870.4051
1341 872.0865 871.0792
1342 872.3752 871.3679
1343 872.4220 871.4147
1344 873.4160 872.4087
1346 876.4234 875.4161
1347 876.4249 875.4176
1348 876.4334 875.4261
1349 438.7390 875.4634
1350 876.4720 875.4647
1351 877.4233 876.4160
1352 877.4319 876.4246
1353 878.4209 877.4136
1354 878.4236 877.4163
1355 439.7278 877.4410
1357 439.7284 877.4422
1358 879.4205 878.4132
1359 879.4621 878.4548
1360 440.2373 878.4600
1361 440.2570 878.4994
1362 880.4385 879.4312
1363 881.4372 880.4299
1364 441.7019 881.3892
1365 882.4008 881.3935
1366 882.4159 881.4086
1367 882.4331 881.4258
1369 883.3798 882.3725
1370 883.4208 882.4135
1375 885.4222 884.4149
1376 885.4244 884.4171
1377 885.4279 884.4206
1378 885.4380 884.4307
1379 887.4221 886.4148
1380 889.4346 888.4273
1381 890.4275 889.4202
1382 891.4252 890.4179
1383 891.4284 890.4211
1386 892.4274 891.4201
1387 892.4382 891.4309
1388 892.4426 891.4353
1389 892.4435 891.4362
1391 892.4639 891.4566
1393 893.4592 892.4519
1394 447.2657 892.5168
1395 894.4297 893.4224
1396 894.4327 893.4254
1397 894.4346 893.4273
1398 895.3980 894.3907
1399 895.4471 894.4398
1401 896.4413 895.4340
1405 449.6996 897.3846
1406 898.4272 897.4199
1407 898.4332 897.4259
1408 898.4398 897.4325
1409 898.4411 897.4338
1410 898.4538 897.4465
1411 899.3743 898.3670
1412 899.4241 898.4168
1413 899.4351 898.4278
1414 450.2336 898.4526
1415 450.2508 898.4870
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