MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1141 770.3792 769.3719
1144 772.3477 771.3404
1145 772.3495 771.3422
1146 772.3765 771.3692
1148 775.3962 774.3889
1149 776.3746 775.3673
1152 779.3672 778.3599
1154 390.2121 778.4096
1155 390.2124 778.4102
1156 781.3699 780.3626
1160 391.2148 780.4150
1161 391.2204 780.4262
1162 782.4020 781.3947
1163 783.3643 782.3570
1167 392.2220 782.4294
1168 392.2220 782.4294
1169 783.4369 782.4296
1172 393.1937 784.3728
1174 786.3975 785.3902
1175 787.8964 786.8891
1176 788.3767 787.3694
1177 789.3704 788.3631
1182 397.6884 793.3622
1183 397.6885 793.3624
1186 398.2097 794.4048
1189 797.3800 796.3727
1190 399.1942 796.3738
1191 797.3969 796.3896
1192 798.3987 797.3914
1193 799.3585 798.3512
1194 799.4008 798.3935
1195 799.4015 798.3942
1196 400.2195 798.4244
1198 800.3812 799.3739
1200 400.7168 799.4190
1203 800.8650 799.8577
1204 801.3735 800.3662
1205 801.3971 800.3898
1206 801.3988 800.3915
1207 801.4275 800.4202
1208 801.4296 800.4223
1209 401.6875 801.3604
1210 802.3902 801.3829
1211 802.8839 801.8766
1212 803.3982 802.3909
1214 404.2101 806.4056
1215 807.4136 806.4063
1217 808.9127 807.9054
1218 810.3633 809.3560
1219 405.6857 809.3568
1220 810.3970 809.3897
1221 811.4062 810.3989
1224 406.7199 811.4252
1225 813.4152 812.4079
1226 814.3959 813.3886
1227 814.3986 813.3913
1228 814.3992 813.3919
1229 815.4052 814.3979
1230 408.6962 815.3778
1232 821.4066 820.3993
1233 821.4207 820.4134
1234 411.2174 820.4202
1235 411.2175 820.4204
1236 411.2361 820.4576
1237 822.4075 821.4002
1239 824.4263 823.4190
1240 824.4268 823.4195
1241 412.7173 823.4200
1242 412.7549 823.4952
1243 824.7479 823.7406
1244 824.9186 823.9113
1245 825.3748 824.3675
1246 825.3994 824.3921
1247 413.2096 824.4046
1251 828.3740 827.3667
1253 828.4359 827.4286
1254 828.4443 827.4370
1256 832.3849 831.3776
1257 834.4061 833.3988
1258 835.4014 834.3941
1259 835.4141 834.4068
1260 418.7227 835.4308
1261 418.7229 835.4312
1264 419.2139 836.4132
1265 839.3895 838.3822
1266 840.4130 839.4057
1268 840.8779 839.8706
1269 841.3685 840.3612
1270 841.3690 840.3617
1271 421.1886 840.3626
1273 421.7043 841.3940
1274 421.7225 841.4304
1275 421.7227 841.4308
1279 842.5131 841.5058
1280 843.4074 842.4001
1281 843.4111 842.4038
1282 843.4167 842.4094
1283 422.2135 842.4124
1284 844.4306 843.4233
1285 845.4252 844.4179
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