MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
962 321.1848 640.3550
965 643.2616 642.2543
966 644.3278 643.3205
973 651.3636 650.3563
974 327.1848 652.3550
975 327.1907 652.3668
978 655.3439 654.3366
979 655.3748 654.3675
983 656.8219 655.8146
988 660.3160 659.3087
989 660.8268 659.8195
990 665.3635 664.3562
991 665.3665 664.3592
992 333.1909 664.3672
994 334.1803 666.3460
995 334.1803 666.3460
996 667.3535 666.3462
998 334.7033 667.3920
999 668.4000 667.3927
1000 671.2988 670.2915
1001 671.2991 670.2918
1002 671.2996 670.2923
1004 337.1797 672.3448
1009 674.3342 673.3269
1011 339.6644 677.3142
1014 678.3377 677.3304
1015 341.1878 680.3610
1016 681.3689 680.3616
1017 341.1887 680.3628
1018 341.2006 680.3866
1019 341.2007 680.3868
1020 341.2007 680.3868
1021 682.3312 681.3239
1023 341.6858 681.3570
1024 342.1899 682.3652
1025 683.3753 682.3680
1026 683.3777 682.3704
1029 687.3336 686.3263
1030 689.3359 688.3286
1031 345.1777 688.3408
1033 690.3502 689.3429
1034 346.2032 690.3918
1036 694.3417 693.3344
1037 695.3361 694.3288
1038 348.2082 694.4018
1041 697.3145 696.3072
1043 349.1983 696.3820
1044 350.1753 698.3360
1053 708.3789 707.3716
1054 354.6933 707.3720
1055 711.4175 710.4102
1056 712.3638 711.3565
1057 358.1847 714.3548
1058 721.3627 720.3554
1059 722.3941 721.3868
1060 361.7010 721.3874
1062 362.7090 723.4034
1064 362.7093 723.4040
1065 726.3427 725.3354
1066 726.3427 725.3354
1067 726.3431 725.3358
1068 363.6808 725.3470
1069 726.3809 725.3736
1070 727.3307 726.3234
1072 730.3378 729.3305
1075 367.1911 732.3676
1076 367.1911 732.3676
1077 734.8580 733.8507
1080 737.8580 736.8507
1081 369.6934 737.3722
1089 742.4113 741.4040
1091 743.3591 742.3518
1092 743.3748 742.3675
1093 743.3964 742.3891
1097 744.3535 743.3462
1098 744.3644 743.3571
1100 746.8679 745.8606
1101 374.1714 746.3282
1102 748.3196 747.3123
1103 376.1909 750.3672
1105 752.3751 751.3678
1107 756.3532 755.3459
1108 756.3535 755.3462
1109 756.3925 755.3852
1110 757.3745 756.3672
1111 757.3755 756.3682
1113 757.4194 756.4121
1114 379.2173 756.4200
1115 379.6939 757.3732
1117 380.2305 758.4464
1118 380.2307 758.4468
1119 380.2308 758.4470
1120 380.2310 758.4474
1121 380.2314 758.4482
1124 380.2322 758.4498
1125 380.2325 758.4504
1126 760.3600 759.3527
1131 764.4368 763.4295
1132 766.8679 765.8606
1135 767.3870 766.3797
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