MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
827 461.2587 460.2514
828 461.2587 460.2514
829 466.2448 465.2375
830 468.2934 467.2861
832 472.2793 471.2720
833 473.2367 472.2294
834 474.2316 473.2243
835 474.2323 473.2250
836 475.2364 474.2291
837 475.2376 474.2303
838 475.2376 474.2303
839 475.2378 474.2305
840 475.2385 474.2312
841 475.2390 474.2317
842 475.2404 474.2331
843 475.2407 474.2334
844 476.2357 475.2284
845 477.1136 476.1063
846 479.2667 478.2594
847 484.2466 483.2393
848 484.7432 483.7359
849 487.2519 486.2446
850 488.1937 487.1864
851 488.2359 487.2286
852 491.2365 490.2292
854 493.0206 492.0133
855 493.0215 492.0142
856 493.0220 492.0147
857 493.0223 492.0150
858 493.0225 492.0152
859 493.0225 492.0152
860 493.0231 492.0158
861 493.0238 492.0165
862 501.2313 500.2240
863 501.2326 500.2253
864 511.2773 510.2700
866 516.2605 515.2532
868 526.2991 525.2918
869 527.2834 526.2761
871 528.7712 527.7639
872 529.2625 528.2552
876 539.3203 538.3130
877 539.3207 538.3134
878 542.2080 541.2007
881 554.3054 553.2981
882 554.3055 553.2982
883 554.3058 553.2985
885 556.3094 555.3021
887 568.2738 567.2665
888 570.7175 569.7102
889 570.7185 569.7112
890 570.7188 569.7115
891 570.7189 569.7116
892 574.9585 573.9512
893 576.3535 575.3462
894 579.2444 578.2371
896 579.3168 578.3095
897 579.3181 578.3108
898 580.3003 579.2930
899 582.2897 581.2824
901 584.3413 583.3340
902 586.2855 585.2782
904 588.2646 587.2573
905 589.2919 588.2846
906 592.2726 591.2653
907 598.2843 597.2770
908 598.3314 597.3241
909 602.2790 601.2717
910 602.2808 601.2735
912 303.8862 605.7578
913 303.8864 605.7582
914 303.8867 605.7588
915 303.8870 605.7594
916 608.3412 607.3339
917 608.3416 607.3343
918 304.8365 607.6584
919 304.8365 607.6584
920 304.8365 607.6584
921 304.8369 607.6592
922 610.2836 609.2763
923 610.2843 609.2770
924 611.7953 610.7880
925 612.3010 611.2937
936 622.3025 621.2952
938 624.3351 623.3278
940 624.3364 623.3291
941 625.3425 624.3352
942 313.1752 624.3358
944 626.2899 625.2826
945 629.2892 628.2819
946 629.2904 628.2831
949 631.7888 630.7815
950 631.9670 630.9597
951 317.6640 633.3134
952 634.3218 633.3145
954 635.3059 634.2986
955 636.3834 635.3761
957 320.1769 638.3392
959 320.1774 638.3402
961 321.1723 640.3300
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