MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
727 425.2355 424.2282
728 425.2369 424.2296
729 425.2369 424.2296
730 425.2371 424.2298
731 425.2373 424.2300
732 425.2377 424.2304
733 425.2889 424.2816
734 425.2903 424.2830
735 426.2075 425.2002
736 426.2076 425.2003
737 426.2409 425.2336
738 426.2421 425.2348
739 427.2542 426.2469
740 427.2543 426.2470
741 427.2550 426.2477
742 428.2012 427.1939
743 428.2546 427.2473
744 428.2554 427.2481
745 428.2556 427.2483
746 428.2560 427.2487
747 428.2563 427.2490
748 428.2564 427.2491
749 428.2620 427.2547
750 430.2303 429.2230
751 430.2305 429.2232
752 430.3025 429.2952
753 432.2205 431.2132
754 437.2007 436.1934
755 437.2011 436.1938
756 437.2013 436.1940
757 437.2017 436.1944
758 437.2018 436.1945
759 437.2025 436.1952
760 438.1989 437.1916
761 439.2142 438.2069
762 439.2162 438.2089
763 439.2165 438.2092
764 439.2166 438.2093
765 439.2166 438.2093
766 439.2172 438.2099
767 439.2174 438.2101
768 439.2178 438.2105
769 439.2182 438.2109
770 439.2185 438.2112
771 439.2186 438.2113
772 439.2188 438.2115
773 439.2190 438.2117
774 440.2877 439.2804
775 441.2089 440.2016
776 441.2109 440.2036
777 441.2297 440.2224
778 441.2319 440.2246
779 441.2323 440.2250
780 442.2350 441.2277
781 442.2351 441.2278
782 442.2355 441.2282
783 442.2360 441.2287
784 442.2365 441.2292
785 442.2426 441.2353
786 443.2460 442.2387
787 443.2478 442.2405
788 443.2478 442.2405
789 443.2478 442.2405
790 443.2481 442.2408
791 443.2485 442.2412
792 443.2490 442.2417
793 443.2499 442.2426
794 443.2500 442.2427
795 444.2826 443.2753
796 444.2827 443.2754
797 446.2251 445.2178
798 446.2620 445.2547
799 447.2768 446.2695
800 449.1515 448.1442
801 451.2510 450.2437
802 455.2101 454.2028
803 455.2113 454.2040
804 455.2115 454.2042
805 455.2115 454.2042
806 455.2116 454.2043
807 455.2119 454.2046
808 455.2129 454.2056
809 455.2132 454.2059
810 455.2136 454.2063
811 457.2250 456.2177
812 457.2273 456.2200
813 457.2274 456.2201
814 457.2274 456.2201
815 457.2274 456.2201
816 457.2278 456.2205
817 457.2281 456.2208
818 457.2286 456.2213
819 457.2291 456.2218
820 457.2294 456.2221
821 457.2298 456.2225
822 458.2378 457.2305
823 459.1028 458.0955
824 461.2566 460.2493
825 461.2584 460.2511
826 461.2585 460.2512
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