MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
627 393.3028 392.2955
628 393.3030 392.2957
629 393.3031 392.2958
630 395.1914 394.1841
631 395.1918 394.1845
632 395.1918 394.1845
633 395.1918 394.1845
634 395.2258 394.2185
635 395.2261 394.2188
636 395.2261 394.2188
637 395.2263 394.2190
638 395.2268 394.2195
639 395.2621 394.2548
640 396.2640 395.2567
641 397.2057 396.1984
642 397.2059 396.1986
643 397.2060 396.1987
644 397.2062 396.1989
645 397.2064 396.1991
646 397.2064 396.1991
647 397.2068 396.1995
648 397.2074 396.2001
649 397.2090 396.2017
650 399.2212 398.2139
651 399.2228 398.2155
652 399.2230 398.2157
653 400.2320 399.2247
654 400.2324 399.2251
655 401.2199 400.2126
656 401.2213 400.2140
657 402.2465 401.2392
658 405.2101 404.2028
659 405.2110 404.2037
660 405.2111 404.2038
661 405.2112 404.2039
662 405.2113 404.2040
663 407.2235 406.2162
664 407.2254 406.2181
665 407.2263 406.2190
666 407.2263 406.2190
667 407.2263 406.2190
668 407.2265 406.2192
669 407.2270 406.2197
670 407.2272 406.2199
671 407.2277 406.2204
672 407.2278 406.2205
673 409.2052 408.1979
674 409.2420 408.2347
675 409.2423 408.2350
676 409.2427 408.2354
677 409.2431 408.2358
678 409.2436 408.2363
679 409.2436 408.2363
680 409.2438 408.2365
681 410.2433 409.2360
682 410.2448 409.2375
683 410.2448 409.2375
684 411.2197 410.2124
685 411.2213 410.2140
686 411.2213 410.2140
687 412.2607 411.2534
688 412.2610 411.2537
689 412.2613 411.2540
690 412.2615 411.2542
691 413.2724 412.2651
692 413.2726 412.2653
693 413.2733 412.2660
694 414.2350 413.2277
695 414.2352 413.2279
696 414.2355 413.2282
697 421.2051 420.1978
698 421.2056 420.1983
699 421.2058 420.1985
700 421.2061 420.1988
701 421.2064 420.1991
702 421.2067 420.1994
703 421.2072 420.1999
704 421.2075 420.2002
705 421.2076 420.2003
706 421.2078 420.2005
707 421.2079 420.2006
708 421.2079 420.2006
709 423.1984 422.1911
710 423.2004 422.1931
711 423.2008 422.1935
712 423.2011 422.1938
713 423.2201 422.2128
714 423.2212 422.2139
715 423.2213 422.2140
716 423.2214 422.2141
717 423.2216 422.2143
718 423.2221 422.2148
719 423.2227 422.2154
720 423.2231 422.2158
721 423.2231 422.2158
722 423.2232 422.2159
723 423.2234 422.2161
724 423.2236 422.2163
725 423.2237 422.2164
726 423.2906 422.2833
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