MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
527 371.3202 370.3129
528 371.3203 370.3130
529 371.3203 370.3130
530 371.3203 370.3130
531 371.3204 370.3131
532 371.3204 370.3131
533 371.3205 370.3132
534 371.3205 370.3132
535 371.3205 370.3132
536 371.3206 370.3133
537 374.2038 373.1965
538 379.1939 378.1866
539 379.1950 378.1877
540 379.1950 378.1877
541 379.1950 378.1877
542 379.1954 378.1881
543 379.1957 378.1884
544 379.1964 378.1891
545 379.1971 378.1898
546 379.1979 378.1906
547 379.2654 378.2581
548 379.6939 378.6866
549 381.1924 380.1851
550 381.1925 380.1852
551 381.1927 380.1854
552 381.1927 380.1854
553 381.1929 380.1856
554 381.1932 380.1859
555 381.1937 380.1864
556 381.2100 380.2027
557 381.2111 380.2038
558 381.2112 380.2039
559 381.2113 380.2040
560 381.2116 380.2043
561 381.2128 380.2055
562 381.2132 380.2059
563 381.2135 380.2062
564 381.2144 380.2071
565 383.1893 382.1820
566 383.1895 382.1822
567 383.1895 382.1822
568 383.1899 382.1826
569 383.1905 382.1832
570 383.1909 382.1836
571 383.1909 382.1836
572 383.2061 382.1988
573 383.2076 382.2003
574 383.2078 382.2005
575 383.2085 382.2012
576 383.2088 382.2015
577 383.2094 382.2021
578 383.2095 382.2022
579 383.2101 382.2028
580 383.2101 382.2028
581 383.2264 382.2191
582 383.2275 382.2202
583 383.2276 382.2203
584 383.2277 382.2204
585 383.2282 382.2209
586 383.2285 382.2212
587 383.2297 382.2224
588 383.2305 382.2232
589 385.2041 384.1968
590 385.2047 384.1974
591 385.2047 384.1974
592 385.2049 384.1976
593 385.2234 384.2161
594 385.2440 384.2367
595 387.2372 386.2299
596 387.2389 386.2316
597 387.2390 386.2317
598 387.2390 386.2317
599 387.2390 386.2317
600 391.2295 390.2222
601 391.2312 390.2239
602 391.2878 390.2805
603 391.2880 390.2807
604 391.2881 390.2808
605 391.2884 390.2811
606 391.2888 390.2815
607 391.2890 390.2817
608 391.2891 390.2818
609 393.2459 392.2386
610 393.2471 392.2398
611 393.2471 392.2398
612 393.2472 392.2399
613 393.2477 392.2404
614 393.2479 392.2406
615 393.2990 392.2917
616 393.3006 392.2933
617 393.3012 392.2939
618 393.3014 392.2941
619 393.3015 392.2942
620 393.3017 392.2944
621 393.3019 392.2946
622 393.3020 392.2947
623 393.3023 392.2950
624 393.3026 392.2953
625 393.3027 392.2954
626 393.3027 392.2954
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