MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
427 361.1838 360.1765
428 361.1844 360.1771
429 363.1794 362.1721
430 363.1808 362.1735
431 363.1809 362.1736
432 363.1811 362.1738
433 363.1814 362.1741
434 363.1819 362.1746
435 363.1819 362.1746
436 363.1821 362.1748
437 363.1824 362.1751
438 363.1996 362.1923
439 363.2004 362.1931
440 363.2025 362.1952
441 365.1784 364.1711
442 365.1798 364.1725
443 365.1801 364.1728
444 365.1804 364.1731
445 365.1804 364.1731
446 365.1821 364.1748
447 365.1959 364.1886
448 365.1969 364.1896
449 365.1969 364.1896
450 365.1970 364.1897
451 365.1973 364.1900
452 365.1974 364.1901
453 365.1977 364.1904
454 365.1982 364.1909
455 365.1983 364.1910
456 365.1989 364.1916
457 365.1996 364.1923
458 365.1997 364.1924
459 365.2000 364.1927
460 365.2144 364.2071
461 365.2160 364.2087
462 365.2164 364.2091
463 365.2166 364.2093
464 365.2172 364.2099
465 365.2181 364.2108
466 365.2186 364.2113
467 365.2192 364.2119
468 365.2198 364.2125
469 367.1929 366.1856
470 367.1938 366.1865
471 367.1938 366.1865
472 367.1939 366.1866
473 367.1952 366.1879
474 367.1956 366.1883
475 367.1959 366.1886
476 367.2113 366.2040
477 367.2125 366.2052
478 367.2128 366.2055
479 367.2129 366.2056
480 367.2132 366.2059
481 367.2133 366.2060
482 367.2141 366.2068
483 367.2153 366.2080
484 367.2153 366.2080
485 367.2329 366.2256
486 367.2331 366.2258
487 367.2335 366.2262
488 367.2340 366.2267
489 367.2351 366.2278
490 369.2107 368.2034
491 369.2275 368.2202
492 369.2282 368.2209
493 369.2282 368.2209
494 369.2283 368.2210
495 369.2285 368.2212
496 369.2287 368.2214
497 369.2291 368.2218
498 369.2298 368.2225
499 369.2467 368.2394
500 369.2488 368.2415
501 369.2492 368.2419
502 370.2309 369.2236
503 370.2317 369.2244
504 370.2318 369.2245
505 370.2324 369.2251
506 370.2501 369.2428
507 370.2518 369.2445
508 371.1892 370.1819
509 371.1899 370.1826
510 371.1900 370.1827
511 371.1905 370.1832
512 371.2442 370.2369
513 371.2447 370.2374
514 371.2635 370.2562
515 371.3168 370.3095
516 371.3172 370.3099
517 371.3180 370.3107
518 371.3180 370.3107
519 371.3190 370.3117
520 371.3191 370.3118
521 371.3191 370.3118
522 371.3192 370.3119
523 371.3193 370.3120
524 371.3196 370.3123
525 371.3196 370.3123
526 371.3199 370.3126
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