MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
327 341.1976 340.1903
328 341.1979 340.1906
329 341.2169 340.2096
330 341.2170 340.2097
331 343.1934 342.1861
332 343.2317 342.2244
333 345.1450 344.1377
334 346.2602 345.2529
335 347.1845 346.1772
336 347.1858 346.1785
337 347.1859 346.1786
338 347.1860 346.1787
339 347.1863 346.1790
340 347.1864 346.1791
341 347.1867 346.1794
342 347.1868 346.1795
343 347.1872 346.1799
344 347.1884 346.1811
345 347.1888 346.1815
346 347.1889 346.1816
347 347.2049 346.1976
348 347.2061 346.1988
349 349.1997 348.1924
350 349.2012 348.1939
351 349.2017 348.1944
352 349.2018 348.1945
353 349.2018 348.1945
354 349.2021 348.1948
355 349.2023 348.1950
356 349.2025 348.1952
357 349.2027 348.1954
358 349.2028 348.1955
359 349.2028 348.1955
360 349.2030 348.1957
361 349.2030 348.1957
362 349.2217 348.2144
363 349.2220 348.2147
364 349.2224 348.2151
365 349.2224 348.2151
366 349.2225 348.2152
367 349.2225 348.2152
368 349.2225 348.2152
369 349.2226 348.2153
370 351.1985 350.1912
371 351.1995 350.1922
372 351.1996 350.1923
373 351.1997 350.1924
374 351.1999 350.1926
375 351.2000 350.1927
376 351.2157 350.2084
377 351.2169 350.2096
378 351.2173 350.2100
379 351.2174 350.2101
380 351.2176 350.2103
381 351.2177 350.2104
382 351.2179 350.2106
383 351.2181 350.2108
384 351.2184 350.2111
385 351.2186 350.2113
386 351.2190 350.2117
387 351.2190 350.2117
388 351.2378 350.2305
389 351.2378 350.2305
390 351.2380 350.2307
391 351.2380 350.2307
392 351.2380 350.2307
393 351.2380 350.2307
394 351.2381 350.2308
395 351.2382 350.2309
396 352.2020 351.1947
397 352.2028 351.1955
398 352.2032 351.1959
399 353.1768 352.1695
400 353.1788 352.1715
401 353.1788 352.1715
402 353.1791 352.1718
403 353.1792 352.1719
404 353.1794 352.1721
405 353.1796 352.1723
406 353.1800 352.1727
407 353.1800 352.1727
408 353.1803 352.1730
409 353.2524 352.2451
410 353.2537 352.2464
411 353.2538 352.2465
412 353.2539 352.2466
413 353.2547 352.2474
414 353.2548 352.2475
415 355.1924 354.1851
416 355.1940 354.1867
417 355.1943 354.1870
418 355.1944 354.1871
419 355.1946 354.1873
420 355.1949 354.1876
421 355.1952 354.1879
422 355.1952 354.1879
423 355.1959 354.1886
424 355.2488 354.2415
425 357.2840 356.2767
426 361.1834 360.1761
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