MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
227 329.1938 328.1865
228 329.1938 328.1865
229 329.2162 328.2089
230 331.1902 330.1829
231 331.1906 330.1833
232 331.1906 330.1833
233 331.1907 330.1834
234 331.1908 330.1835
235 331.1909 330.1836
236 331.1910 330.1837
237 331.1913 330.1840
238 331.2109 330.2036
239 331.2109 330.2036
240 331.2113 330.2040
241 333.2049 332.1976
242 333.2061 332.1988
243 333.2065 332.1992
244 333.2065 332.1992
245 333.2067 332.1994
246 333.2068 332.1995
247 333.2071 332.1998
248 333.2073 332.2000
249 333.2076 332.2003
250 333.2077 332.2004
251 333.2078 332.2005
252 333.2078 332.2005
253 333.2247 332.2174
254 333.2271 332.2198
255 333.2271 332.2198
256 333.2274 332.2201
257 333.2276 332.2203
258 335.1865 334.1792
259 335.1866 334.1793
260 335.1869 334.1796
261 335.1873 334.1800
262 335.1874 334.1801
263 335.2205 334.2132
264 335.2218 334.2145
265 335.2223 334.2150
266 335.2223 334.2150
267 335.2223 334.2150
268 335.2226 334.2153
269 335.2228 334.2155
270 335.2232 334.2159
271 335.2232 334.2159
272 335.2234 334.2161
273 335.2235 334.2162
274 335.2237 334.2164
275 335.2402 334.2329
276 335.2428 334.2355
277 335.2428 334.2355
278 335.2431 334.2358
279 335.2431 334.2358
280 335.2436 334.2363
281 337.1829 336.1756
282 337.1836 336.1763
283 337.1836 336.1763
284 337.1837 336.1764
285 337.1840 336.1767
286 337.1845 336.1772
287 337.1852 336.1779
288 337.1856 336.1783
289 337.1862 336.1789
290 337.1871 336.1798
291 337.2013 336.1940
292 337.2013 336.1940
293 337.2013 336.1940
294 337.2015 336.1942
295 337.2018 336.1945
296 337.2019 336.1946
297 337.2020 336.1947
298 337.2569 336.2496
299 337.2576 336.2503
300 338.1675 337.1602
301 338.2401 337.2328
302 338.2413 337.2340
303 338.2413 337.2340
304 338.2416 337.2343
305 338.2417 337.2344
306 338.2418 337.2345
307 339.1608 338.1535
308 339.1621 338.1548
309 339.1634 338.1561
310 339.1640 338.1567
311 339.1640 338.1567
312 339.1653 338.1580
313 339.1986 338.1913
314 339.2018 338.1945
315 339.2743 338.2670
316 341.1476 340.1403
317 341.1772 340.1699
318 341.1797 340.1724
319 341.1815 340.1742
320 341.1817 340.1744
321 341.1960 340.1887
322 341.1965 340.1892
323 341.1967 340.1894
324 341.1968 340.1895
325 341.1971 340.1898
326 341.1973 340.1900
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