MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Quantitation overview (24 proteins)

Score Mass Matches Sequences emPAI
Score Mass Matches Sequences emPAI
1.1 ENO1_YEAST 375 46844 13 (10) 8 (8) 0.85 Enolase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO1 PE=1 SV=3
1.2 ENO2_YEAST 332 46942 12 (9) 7 (7) 0.72 Enolase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO2 PE=1 SV=2
2 PGK_YEAST 294 44768 9 (9) 8 (8) 0.90 Phosphoglycerate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PGK1 PE=1 SV=2
3.1 G3P1_YEAST 219 35842 7 (7) 5 (5) 0.70 Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH1 PE=1 SV=3
3.2 G3P2_YEAST 182 35938 5 (5) 3 (3) 0.42 Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH2 PE=1 SV=3
4 SCW4_YEAST 152 40434 2 (2) 1 (1) 0.08 Probable family 17 glucosidase SCW4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SCW4 PE=1 SV=1
5 HSP26_YEAST 103 23865 2 (2) 2 (2) 0.30 Heat shock protein 26 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP26 PE=1 SV=3
6 A0A8H6ZX18_PLEOS 79 37983 2 (2) 1 (1) 0.09 Peroxidase OS=Pleurotus ostreatus OX=5322 GN=VPL1_1 PE=3 SV=1
7 Q9FD70_ENTFL 66 86614 1 (1) 1 (1) 0.04 Acetyl-CoA acetyltransferase/HMG-CoA reductase OS=Enterococcus faecalis GN=mvaE PE=3 SV=1
8 SDO1L_YEAST 61 12002 3 (3) 3 (3) 1.13 Restriction of telomere capping protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RTC3 PE=1 SV=1
9 PIR1_YEAST 53 34829 1 (1) 1 (1) 0.10 Cell wall mannoprotein PIR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PIR1 PE=1 SV=1
10 HSP12_YEAST 50 11686 2 (2) 2 (2) 0.68 12 kDa heat shock protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP12 PE=1 SV=1
11 PMG1_YEAST 49 27592 1 (1) 1 (1) 0.12 Phosphoglycerate mutase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GPM1 PE=1 SV=3
12 CYPH_YEAST 46 17494 1 (1) 1 (1) 0.19 Peptidyl-prolyl cis-trans isomerase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CPR1 PE=1 SV=3
13 EXG1_YEAST 41 51735 2 (1) 2 (1) 0.06 Glucan 1,3-beta-glucosidase I/II OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=EXG1 PE=1 SV=1
14 PST2_YEAST 41 21010 1 (1) 1 (1) 0.16 Protoplast secreted protein 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PST2 PE=1 SV=1
15 COFI_YEAST 36 15948 1 (1) 1 (1) 0.21 Cofilin OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=COF1 PE=1 SV=1
16 NACB2_YEAST 36 16724 1 (1) 1 (1) 0.20 Nascent polypeptide-associated complex subunit beta-2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BTT1 PE=1 SV=1
17 ATP7_YEAST 35 0 1 (1) 1 (1) 0.17 description
18 FAB1_YEAST 24 258743 3 (1) 1 (1) 0.01 1-phosphatidylinositol 3-phosphate 5-kinase FAB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=FAB1 PE=1 SV=3
19 B211p2_002|NRPSb 24 633771 2 (1) 2 (1) 0.01 OS=Anaeromyces robustus OX=1754192 PE=2 SV=1
20 PDC1_YEAST 19 61685 5 (2) 4 (1) 0.05 Pyruvate decarboxylase isozyme 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PDC1 PE=1 SV=7
21 YHM2_YEAST 17 34334 1 (1) 1 (1) 0.10 Citrate/oxoglutarate carrier protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YHM2 PE=1 SV=1
22 SERC_YEAST 16 43560 2 (1) 1 (1) 0.08 Phosphoserine aminotransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SER1 PE=1 SV=1

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