MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 4709)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1051 352.2036 702.3926 702.3912 2.08 0 20 0.15 +1Score > 25 indicates identity VASLADK
935 618.3306 617.3233 617.3207 4.28 0 19 0.15 +1Score > 23 indicates identity MGGLPK + Oxidation (M)
1786 508.7574 1015.5002 1015.4934 6.74 0 16 0.16 +1Score > 21 indicates identity
Score > 20 indicates homology		 GLNNGENVAK + Deamidated (NQ)
1587 478.2524 954.4902 954.4811 9.62 1 16 0.13 +1Score > 20 indicates identity FKFEDAAK
867 522.2198 521.2125 521.2155 -5.80 0 16 0.072 +1Score > 17 indicates identity SVMGE
1231 409.7256 817.4366 817.4334 3.98 0 15 0.16 +1Score > 22 indicates identity
Score > 20 indicates homology		 VDPFAAAK
1074 366.2089 730.4032 730.3974 8.07 0 15 0.35 +1Score > 23 indicates identity LGQTVGR + Deamidated (NQ)
4204 797.4376 2389.2910 2389.3104 -8.13 0 15 0.11 +1Score > 18 indicates identity LQVHLLENNNDLDLTIGLLLK + 2 Deamidated (NQ)
1116 380.2043 758.3940 758.3922 2.38 0 14 0.11 +1Score > 21 indicates identity
Score > 17 indicates homology		 QQLNEK
865 515.3315 514.3242 514.3227 2.92 0 14 0.4 +1Score > 22 indicates identity AVAVR
1127 380.7147 759.4148 759.4239 -11.9 1 14 0.13 +1Score > 24 indicates identity
Score > 18 indicates homology		 RISQEK
1047 701.3586 700.3513 700.3504 1.36 0 14 0.14 +1Score > 17 indicates identity INPSNR + Deamidated (NQ)
1008 673.4253 672.4180 672.4170 1.51 0 13 0.28 +1Score > 20 indicates identity KPSITK
1813 512.2475 1022.4804 1022.4669 13.3 0 13 0.062 +1Score > 22 indicates identity
Score > 14 indicates homology		 LGGENNYEK
2579 633.8265 1265.6384 1265.6140 19.4 0 13 0.48 +1Score > 23 indicates identity VESGLFSVDGEK
1006 337.2161 672.4176 672.4170 0.95 1 13 0.3 +1Score > 20 indicates identity VAKDLK
968 322.6721 643.3296 643.3289 1.11 0 13 0.16 +1Score > 18 indicates identity LGSPDR
2802 734.8577 1467.7008 1467.6994 1.01 1 13 0.29 +1Score > 22 indicates identity
Score > 20 indicates homology		 AIPNNSFRDSFAK + 2 Deamidated (NQ)
2152 1105.5758 1104.5685 1104.5676 0.84 1 13 0.54 +1Score > 23 indicates identity RDGPASFSLR
2397 583.7769 1165.5392 1165.5397 -0.39 0 13 0.13 +1Score > 22 indicates identity
Score > 16 indicates homology		 NINLSEMSSR + Oxidation (M)
2454 590.2869 1178.5592 1178.5390 17.2 0 12 0.17 +1Score > 22 indicates identity
Score > 17 indicates homology		 AAFTPEMQER
1766 504.7656 1007.5166 1007.5036 13.0 0 12 0.49 +1Score > 21 indicates identity ILNNESYR
2776 724.3881 1446.7616 1446.7579 2.58 0 12 0.57 +1Score > 22 indicates identity LGATIDVEHSHLR
1392 446.7362 891.4578 891.4524 6.10 0 12 0.4 +1Score > 23 indicates identity
Score > 20 indicates homology		 IPQFMEK
967 644.3367 643.3294 643.3289 0.76 0 12 0.21 +1Score > 18 indicates identity LGSPDR
1007 337.2162 672.4178 672.4170 1.25 1 12 0.41 +1Score > 20 indicates identity VAKDLK
2599 640.8170 1279.6194 1279.6329 -10.5 1 12 0.74 +1Score > 23 indicates identity KTSQLMEEIGK + Deamidated (NQ); Oxidation (M)
886 556.3214 555.3141 555.3241 -18.0 0 12 0.11 +1Score > 15 indicates identity AGRPR
1238 411.7278 821.4410 821.4317 11.4 1 12 0.48 +1Score > 21 indicates identity ETMKLGK + Oxidation (M)
1533 470.7669 939.5192 939.5066 13.5 0 12 0.27 +1Score > 18 indicates identity FLGFDTIK
1138 767.4058 766.3985 766.3973 1.57 0 12 0.41 +1Score > 20 indicates identity KPEQHK + Deamidated (NQ)
980 655.3778 654.3705 654.3701 0.69 0 11 0.1 +1Score > 14 indicates identity SVPAPGK
2931 775.3744 1548.7342 1548.7341 0.11 0 11 0.11 +1Score > 22 indicates identity
Score > 14 indicates homology		 MSTIKPSPSNNNLK + 3 Deamidated (NQ); Oxidation (M)
2775 724.3838 1446.7530 1446.7579 -3.37 0 11 0.73 +1Score > 22 indicates identity LGATIDVEHSHLR
2774 724.3837 1446.7528 1446.7579 -3.51 0 11 0.74 +1Score > 22 indicates identity LGATIDVEHSHLR
2833 745.8605 1489.7064 1489.6783 18.9 0 11 0.69 +1Score > 22 indicates identity AVEALNDSELNGEK + 2 Deamidated (NQ)
970 644.3370 643.3297 643.3289 1.23 0 11 0.26 +1Score > 18 indicates identity LGSPDR
2304 573.2858 1144.5570 1144.5724 -13.4 0 11 0.17 +1Score > 22 indicates identity
Score > 16 indicates homology		 ENGLLNEISR + Deamidated (NQ)
1675 984.4705 983.4632 983.4784 -15.5 0 11 0.47 +1Score > 20 indicates identity NPSQRPQR + 2 Deamidated (NQ)
971 324.1721 646.3296 646.3286 1.65 0 11 0.79 +1Score > 24 indicates identity
Score > 22 indicates homology		 LSGNGAK + Deamidated (NQ)
3397 931.9835 1861.9524 1861.9469 3.00 0 11 0.67 +1Score > 22 indicates identity NTVLDLLSVMTHPHNR + Oxidation (M)
930 616.3687 615.3614 615.3592 3.68 0 11 0.18 +1Score > 25 indicates identity
Score > 16 indicates homology		 AVIEGK
879 542.2944 541.2871 541.2972 -18.7 0 11 0.097 +1Score > 13 indicates identity QPAAR
2641 664.8293 1327.6440 1327.6408 2.45 0 11 0.13 +1Score > 21 indicates identity
Score > 14 indicates homology		 NSATILNYFER + Deamidated (NQ)
2709 700.3983 1398.7820 1398.7653 12.0 1 11 0.41 +1Score > 19 indicates identity QPNLKCIVLTGR + Deamidated (NQ)
1179 397.2276 792.4406 792.4381 3.16 0 11 0.43 +1Score > 21 indicates identity
Score > 19 indicates homology		 LSFEIGK
870 528.3152 527.3079 527.3067 2.23 0 10 0.16 +1Score > 15 indicates identity QGVPK
1010 338.1772 674.3398 674.3421 -3.41 0 10 0.15 +1Score > 22 indicates identity
Score > 15 indicates homology		 TCLGPK
1524 470.2354 938.4562 938.4570 -0.77 0 10 0.19 +1Score > 20 indicates identity
Score > 16 indicates homology		 QENLHNGK
1447 911.4630 910.4557 910.4509 5.35 0 10 0.46 +1Score > 19 indicates identity GQNPVDGPK
3558 991.5044 1980.9942 1980.9792 7.58 1 10 0.75 +1Score > 22 indicates identity NLDTFLNQLRDFLNEK + 2 Deamidated (NQ)
1046 351.1829 700.3512 700.3504 1.25 0 10 0.3 +1Score > 17 indicates identity INPSNR + Deamidated (NQ)
2212 1122.5078 1121.5005 1121.5209 -18.2 0 10 0.2 +1Score > 21 indicates identity
Score > 16 indicates homology		 AGGEGMCIAIK + Oxidation (M)
1429 906.4340 905.4267 905.4277 -1.03 0 10 0.23 +1Score > 23 indicates identity
Score > 16 indicates homology		 AVPSSSACK
2718 705.8547 1409.6948 1409.7006 -4.11 1 10 0.17 +1Score > 21 indicates identity
Score > 15 indicates homology		 NTNISIMRLGMK + Deamidated (NQ); 2 Oxidation (M)
1584 477.7494 953.4842 953.4679 17.2 1 10 0.52 +1Score > 20 indicates identity EPNHSRSK
1277 421.7600 841.5054 841.5021 3.94 0 10 0.42 +1Score > 19 indicates identity GNVIILGR + Deamidated (NQ)
4139 1165.5553 2329.0960 2329.1260 -12.8 0 10 0.77 +1Score > 21 indicates identity MSDNLLSLENPVVPSHYELR + Deamidated (NQ); Oxidation (M)
3414 624.6148 1870.8226 1870.8405 -9.57 1 10 0.48 +1Score > 19 indicates identity NENKVNNATNATHNNSK + 2 Deamidated (NQ)
1178 397.2273 792.4400 792.4381 2.41 0 10 0.68 +1Score > 22 indicates identity
Score > 21 indicates homology		 LSFEIGK
4077 762.7321 2285.1745 2285.1831 -3.77 1 10 0.81 +1Score > 21 indicates identity EFVKLEQSLVLYFGDQLEK + Deamidated (NQ)
2303 573.2856 1144.5566 1144.5724 -13.7 0 10 0.27 +1Score > 21 indicates identity
Score > 17 indicates homology		 ENGLLNEISR + Deamidated (NQ)
4272 830.0791 2487.2155 2487.1675 19.3 1 10 0.81 +1Score > 21 indicates identity FVNMRTGVAGFNDSLMNHLYGK + Deamidated (NQ); Oxidation (M)
2593 639.3292 1276.6438 1276.6636 -15.5 1 10 1 +1Score > 22 indicates identity
Score > 22 indicates homology		 SPTNNNPRIHK
2809 737.8656 1473.7166 1473.7212 -3.08 1 10 0.7 +1Score > 22 indicates identity
Score > 21 indicates homology		 KDHGDVSNQLYAK
3394 930.4912 1858.9678 1858.9523 8.36 1 10 0.75 +1Score > 21 indicates identity LQNSKSLPISEIEASNK + 2 Deamidated (NQ)
1561 949.4656 948.4583 948.4698 -12.2 0 9 1 +1Score > 23 indicates identity
Score > 22 indicates homology		 VAAMSVAQR + Deamidated (NQ); Oxidation (M)
1157 781.3949 780.3876 780.3766 14.1 0 9 0.37 +1Score > 21 indicates identity
Score > 18 indicates homology		 PAPQDPR + Deamidated (NQ)
3061 544.6071 1630.7995 1630.7910 5.18 1 9 1 +1Score > 23 indicates identity
Score > 22 indicates homology		 NSTNASVAGNISNPKR + 2 Deamidated (NQ)
4582 790.3961 3157.5553 3157.6118 -17.9 0 9 0.75 +1Score > 21 indicates identity QDLLPPDINATNGKPLSMAPFLNLFGTTR + Deamidated (NQ); Oxidation (M)
1073 366.1823 730.3500 730.3609 -14.9 0 9 0.51 +1Score > 19 indicates identity NGIGQNK + Deamidated (NQ)
3763 695.3452 2083.0138 2083.0520 -18.4 1 9 1 +1Score > 23 indicates identity
Score > 22 indicates homology		 LNSFISVAAQLAMERFNR + Deamidated (NQ); Oxidation (M)
2368 1160.5620 1159.5547 1159.5770 -19.2 0 9 0.83 +1Score > 21 indicates identity MVPTMFPPPK + Oxidation (M)
3375 926.4229 1850.8312 1850.8396 -4.54 1 9 0.7 +1Score > 20 indicates identity QGLEGNSYKFLFGDMK + 2 Deamidated (NQ); Oxidation (M)
964 641.3636 640.3563 640.3544 2.98 0 9 0.24 +1Score > 16 indicates identity NPSVPK
1090 371.7096 741.4046 741.4021 3.46 0 9 0.37 +1Score > 17 indicates identity QLQQPK + Deamidated (NQ)
3976 1097.0386 2192.0626 2192.0566 2.78 1 9 0.26 +1Score > 22 indicates identity
Score > 16 indicates homology		 DNVDVNGRLGTNMIVQGVMK + Deamidated (NQ); 2 Oxidation (M)
2957 781.9277 1561.8408 1561.8239 10.8 0 9 0.48 +1Score > 22 indicates identity
Score > 18 indicates homology		 ATLSTLFQELNVPK + 2 Deamidated (NQ)
1558 474.7436 947.4726 947.4560 17.6 1 9 0.31 -1Score > 22 indicates identity
Score > 16 indicates homology		 SGPSKTDEK
2118 550.2742 1098.5338 1098.5557 -19.9 0 9 0.7 +1Score > 20 indicates identity AELENLDAPK
1287 423.2225 844.4304 844.4403 -11.6 1 9 0.2 +1Score > 22 indicates identity
Score > 14 indicates homology		 EGGREGLK
2623 655.8193 1309.6240 1309.6013 17.4 0 9 0.98 +1Score > 21 indicates identity EYCPQFQPIK + Deamidated (NQ)
2925 773.9057 1545.7968 1545.8012 -2.79 1 9 1 +1Score > 22 indicates identity
Score > 21 indicates homology		 VASHKNSHNPSLVR + Deamidated (NQ)
1944 1056.5338 1055.5265 1055.5400 -12.8 1 9 0.32 +1Score > 20 indicates identity
Score > 16 indicates homology		 RSGTFFNVK + Deamidated (NQ)
1286 845.4367 844.4294 844.4402 -12.8 1 9 0.87 +1Score > 22 indicates identity
Score > 21 indicates homology		 NARENIK + Deamidated (NQ)
3329 911.8909 1821.7672 1821.8030 -19.6 1 9 0.35 +1Score > 17 indicates identity AVQHKVYNNHNSHDR + 4 Deamidated (NQ)
1136 767.4056 766.3983 766.3973 1.30 0 9 0.5 +1Score > 20 indicates identity
Score > 18 indicates homology		 KPEQHK + Deamidated (NQ)
1147 387.6913 773.3680 773.3820 -18.1 0 9 0.52 +1Score > 18 indicates identity TSPQWR
1137 384.2065 766.3984 766.3861 16.1 0 9 0.59 +1Score > 20 indicates identity
Score > 19 indicates homology		 QLNYTK + Deamidated (NQ)
2617 651.8715 1301.7284 1301.7455 -13.1 1 9 0.69 +1Score > 20 indicates identity SLDLYLAPRVR
1250 414.2173 826.4200 826.4185 1.91 0 9 0.66 +1Score > 19 indicates identity DPPSNGLK
1418 450.2702 898.5258 898.5283 -2.72 1 9 0.53 +1Score > 18 indicates identity ARLMRPR
2910 769.8614 1537.7082 1537.7195 -7.29 1 9 1 +1Score > 21 indicates identity
Score > 21 indicates homology		 KPTNQDYRMQQK + 2 Deamidated (NQ)
1165 783.4008 782.3935 782.3963 -3.53 0 9 0.89 +1Score > 21 indicates identity
Score > 21 indicates homology		 NGFFGLK + Deamidated (NQ)
1541 472.2272 942.4398 942.4559 -17.0 0 8 0.43 +1Score > 17 indicates identity LQQPWNR + 2 Deamidated (NQ)
943 313.1875 624.3604 624.3707 -16.5 1 8 0.55 +1Score > 18 indicates identity VKHNK
2955 521.6174 1561.8304 1561.8028 17.7 1 8 0.6 +1Score > 22 indicates identity
Score > 19 indicates homology		 GILNYYKLATNYK + 2 Deamidated (NQ)
932 309.6664 617.3182 617.3133 8.08 1 8 0.41 +1Score > 23 indicates identity
Score > 17 indicates homology		 KNGNGK + Deamidated (NQ)
3881 712.3528 2134.0366 2134.0695 -15.4 1 8 1 +1Score > 23 indicates identity
Score > 21 indicates homology		 LGQGTFGEVYKGIHLETQR + 2 Deamidated (NQ)
1879 1042.5194 1041.5121 1041.5277 -14.9 0 8 0.18 +1Score > 20 indicates identity
Score > 13 indicates homology		 MEIHSNLAK
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