MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 4 |
| MS data file | : | PRT1245_Other_P1_B12.mgf |
| Database | : | S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues) |
| Timestamp | : | 24 Jun 2025 at 21:22:18 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,772 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ENO1_YEAST | 375 | Enolase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO1 PE=1 SV=3 | |
| 2 | ENO2_YEAST | 332 | Enolase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO2 PE=1 SV=2 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PGK_YEAST | 294 | Phosphoglycerate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PGK1 PE=1 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | G3P1_YEAST | 219 | Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH1 PE=1 SV=3 | |
| 2 | G3P2_YEAST | 182 | Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH2 PE=1 SV=3 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SCW4_YEAST | 152 | Probable family 17 glucosidase SCW4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SCW4 PE=1 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HSP26_YEAST | 103 | Heat shock protein 26 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP26 PE=1 SV=3 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | A0A8H6ZX18_PLEOS | 79 | Peroxidase OS=Pleurotus ostreatus OX=5322 GN=VPL1_1 PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q9FD70_ENTFL | 66 | Acetyl-CoA acetyltransferase/HMG-CoA reductase OS=Enterococcus faecalis GN=mvaE PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SDO1L_YEAST | 61 | Restriction of telomere capping protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RTC3 PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PIR1_YEAST | 53 | Cell wall mannoprotein PIR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PIR1 PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 9.1 | PIR1_YEAST |
53 | 34829 | 1 (1) | 1 (1) | 0.10 | |
| Cell wall mannoprotein PIR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PIR1 PE=1 SV=1 | |||||||
| 4 samesets of PIR1_YEAST | |||||||
| CIS3_YEAST |
53 | 23569 | 1 (1) | 1 (1) | 0.14 | ||
| Cell wall mannoprotein CIS3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CIS3 PE=1 SV=1 | |||||||
| PIR3_YEAST |
53 | 33213 | 1 (1) | 1 (1) | 0.10 | ||
| Cell wall mannoprotein PIR3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PIR3 PE=1 SV=3 | |||||||
| PIR5_YEAST |
53 | 30425 | 1 (1) | 1 (1) | 0.11 | ||
| Cell wall protein PIR5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PIR5 PE=2 SV=2 | |||||||
| HS150_YEAST |
53 | 41263 | 1 (1) | 1 (1) | 0.08 | ||
| Cell wall mannoprotein HSP150 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP150 PE=1 SV=2 | |||||||
1 peptide matches (1 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
D:\Xcalibur\Data\Yan\PRT1245 redo\PRT1245_Other_P1_B12.rawScore > 22 indicates identity |
|||||||||||
| 1255 |
415.2491 | 828.4836 | 828.4817 | 2.31 | 0 | 53 | 4.2e-005 | 1Score > 22 indicates identity |
U | R.IGSIVANR.Q | |
| -11.2 | 1 | 8 | 1.5 | 2 | LGLNRTR | ||||||
| 2.31 | 0 | 6 | 2.2 | 3 | GLSANLVR | ||||||
| 2.33 | 1 | 5 | 2.6 | 4 | LNLEKGR | ||||||
| -11.2 | 1 | 4 | 3.3 | 5 | GILSQRR | ||||||
| 2.33 | 1 | 3 | 4.3 | 6 | LDKLNAR | ||||||
| 15.9 | 0 | 3 | 4.6 | 7 | EQILQAK | ||||||
| 14.3 | 1 | 1 | 6.5 | 8 | LWAQRR | ||||||
| 2.33 | 1 | 0 | 8.4 | 9 | IQNLGRK + Deamidated (NQ) | ||||||
| 2.33 | 1 | 0 | 8.4 | 9 | IQNLGRK + Deamidated (NQ) | ||||||
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HSP12_YEAST | 50 | 12 kDa heat shock protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP12 PE=1 SV=1 |
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