MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 22)

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+1

Accession Score Description
Family member distances as a dendrogram 1 ENO1_YEAST 375 Enolase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO1 PE=1 SV=3
2 ENO2_YEAST 332 Enolase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO2 PE=1 SV=2

+2

Accession Score Description
1 PGK_YEAST 294 Phosphoglycerate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PGK1 PE=1 SV=2

-3

Accession Score Description
Family member distances as a dendrogram 1 G3P1_YEAST 219 Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH1 PE=1 SV=3
2 G3P2_YEAST 182 Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH2 PE=1 SV=3
Cut threshold

Score Mass Matches Sequences emPAI
G3P1_YEAST 219 35842 7 (7) 5 (5) 0.70
Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH1 PE=1 SV=3
G3P2_YEAST 182 35938 5 (5) 3 (3) 0.42
Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH2 PE=1 SV=3
1 sameset of G3P2_YEAST
G3P3_YEAST 182 35838 5 (5) 3 (3) 0.42
Glyceraldehyde-3-phosphate dehydrogenase 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH3 PE=1 SV=3

-8 peptide matches (7 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
1371   442.2690 882.5234 882.5175 6.79 0 23 0.0088 +1Score > 15 indicates identity U X X K.VLPELQGK.L
2164   370.2182 1107.6328 1107.6288 3.57 0 42 0.00031 +1Score > 19 indicates identity U X R.VVDLVEHVAK.A
D:\Xcalibur\Data\Yan\PRT1245 redo\PRT1245_Other_P1_B12.raw

Score > 18 indicates identity
2318   574.8405 1147.6664 1147.6489 15.3 0 43 0.00019 -1Score > 18 indicates identity U X R.VVDLIEYVAK.A
No other peptide matches in query
2507   599.3287 1196.6428 1196.6190 20.0 0 38 0.0011 +1Score > 21 indicates identity U X R.DPANLPWGSLK.I
2563   625.8410 1249.6674 1249.6554 9.61 0 40 0.001 +1Score > 23 indicates identity U X K.IDVAVDSTGVFK.E
2803   490.9587 1469.8543 1469.8342 13.7 0 37 0.00035 +1Score > 15 indicates identity U X X R.VPTVDVSVVDLTVK.L
2805 +1 735.9349 1469.8552 1469.8342 14.4 0 77 4.1e-008 +1Score > 15 indicates identity U X X R.VPTVDVSVVDLTVK.L

+4

Accession Score Description
1 SCW4_YEAST 152 Probable family 17 glucosidase SCW4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SCW4 PE=1 SV=1

+5

Accession Score Description
1 HSP26_YEAST 103 Heat shock protein 26 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP26 PE=1 SV=3

+6

Accession Score Description
1 A0A8H6ZX18_PLEOS 79 Peroxidase OS=Pleurotus ostreatus OX=5322 GN=VPL1_1 PE=3 SV=1

+7

Accession Score Description
1 Q9FD70_ENTFL 66 Acetyl-CoA acetyltransferase/HMG-CoA reductase OS=Enterococcus faecalis GN=mvaE PE=3 SV=1

+8

Accession Score Description
1 SDO1L_YEAST 61 Restriction of telomere capping protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RTC3 PE=1 SV=1

+9

Accession Score Description
1 PIR1_YEAST 53 Cell wall mannoprotein PIR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PIR1 PE=1 SV=1

+10

Accession Score Description
1 HSP12_YEAST 50 12 kDa heat shock protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP12 PE=1 SV=1
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