MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 4
MS data file : PRT1245_Other_P1_B12.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:22:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,772

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 22)

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-1

Accession Score Description
Family member distances as a dendrogram 1 ENO1_YEAST 375 Enolase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO1 PE=1 SV=3
2 ENO2_YEAST 332 Enolase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO2 PE=1 SV=2
Cut threshold

Score Mass Matches Sequences emPAI
ENO1_YEAST 375 46844 13 (10) 8 (8) 0.85
Enolase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO1 PE=1 SV=3
ENO2_YEAST 332 46942 12 (9) 7 (7) 0.72
Enolase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO2 PE=1 SV=2

-16 peptide matches (11 non-duplicate, 5 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
D:\Xcalibur\Data\Yan\PRT1245 redo\PRT1245_Other_P1_B12.raw

Score > 17 indicates identity
1199   400.6969 799.3792 799.3752 5.05 0 26 0.0068 -1Score > 17 indicates identity U X X K.YDLDFK.N
5.07 0 26 0.0068 1 YDLNFK   + Deamidated (NQ)
5.07 0 8 0.45 3 IDNYFK   + Deamidated (NQ)
-8.96 0 7 0.63 4 YNYAIR   + Deamidated (NQ)
5.07 0 4 1.2 5 YIDFNK   + Deamidated (NQ)
5.09 0 4 1.2 5 YINFNK   + 2 Deamidated (NQ)
-13.2 1 3 1.6 7 YNTMKK   + Oxidation (M)
2605   644.3618 1286.7090 1286.6870 17.1 0 32 0.0043 +1Score > 21 indicates identity U X K.NVNDVIAPAFVK.A + Deamidated (NQ)
3241   585.9965 1754.9677 1754.9414 14.9 0 62 2.3e-006 +1Score > 18 indicates identity U X K.TAGIQIVADDLTVTNPK.R
3325 +1 911.4756 1820.9366 1820.9156 11.5 0 88 6.3e-009 +1Score > 22 indicates identity
Score > 18 indicates homology		 U X X R.SGETEDTFIADLVVGLR.T
3326   607.9862 1820.9368 1820.9156 11.6 0 28 0.0065 +1Score > 22 indicates identity
Score > 19 indicates homology		 U X X R.SGETEDTFIADLVVGLR.T
3359   920.9601 1839.9056 1839.9149 -5.02 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology		 U X X R.SIVPSGASTGVHEALEMR.D
3360   614.3169 1839.9289 1839.9149 7.60 0 64 3.3e-006 +1Score > 22 indicates identity U X X R.SIVPSGASTGVHEALEMR.D
3385   928.0125 1854.0104 1853.9847 13.9 0 30 0.0033 +1Score > 18 indicates identity U X K.TAGIQIVADDLTVTNPAR.I
4609   1086.8846 3257.6320 3257.5939 11.7 0 99 9.5e-010 +1Score > 21 indicates identity U X X R.YGASAGNVGDEGGVAPNIQTAEEALDLIVDAIK.A + Deamidated (NQ)

1 subset or intersection (3 subset proteins in total)

Score Mass Subset of
ERR1_YEAST 26 47697 1.1
Enolase-related protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ERR1 PE=3 SV=1
2 samesets of ERR1_YEAST
ERR3_YEAST 26 47682
Enolase-related protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ERR3 PE=3 SV=2
ERR2_YEAST 26 47697
Enolase-related protein 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ERR2 PE=3 SV=1

+2

Accession Score Description
1 PGK_YEAST 294 Phosphoglycerate kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PGK1 PE=1 SV=2

+3

Accession Score Description
Family member distances as a dendrogram 1 G3P1_YEAST 219 Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH1 PE=1 SV=3
2 G3P2_YEAST 182 Glyceraldehyde-3-phosphate dehydrogenase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TDH2 PE=1 SV=3

+4

Accession Score Description
1 SCW4_YEAST 152 Probable family 17 glucosidase SCW4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SCW4 PE=1 SV=1

+5

Accession Score Description
1 HSP26_YEAST 103 Heat shock protein 26 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP26 PE=1 SV=3

+6

Accession Score Description
1 A0A8H6ZX18_PLEOS 79 Peroxidase OS=Pleurotus ostreatus OX=5322 GN=VPL1_1 PE=3 SV=1

+7

Accession Score Description
1 Q9FD70_ENTFL 66 Acetyl-CoA acetyltransferase/HMG-CoA reductase OS=Enterococcus faecalis GN=mvaE PE=3 SV=1

+8

Accession Score Description
1 SDO1L_YEAST 61 Restriction of telomere capping protein 3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RTC3 PE=1 SV=1

+9

Accession Score Description
1 PIR1_YEAST 53 Cell wall mannoprotein PIR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PIR1 PE=1 SV=1

+10

Accession Score Description
1 HSP12_YEAST 50 12 kDa heat shock protein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HSP12 PE=1 SV=1
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