MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
209 323.1700 322.1627
210 323.1849 322.1776
211 323.1856 322.1783
212 323.1858 322.1785
213 323.1858 322.1785
214 323.1859 322.1786
215 323.1861 322.1788
216 323.1862 322.1789
217 323.1864 322.1791
218 323.1865 322.1792
219 323.1869 322.1796
220 323.1870 322.1797
221 323.1873 322.1800
222 323.1885 322.1812
223 323.2030 322.1957
224 323.2048 322.1975
225 323.2058 322.1985
226 323.2060 322.1987
227 325.1824 324.1751
228 325.1834 324.1761
229 325.1835 324.1762
230 325.1836 324.1763
231 325.1836 324.1763
232 325.1844 324.1771
233 325.1844 324.1771
234 325.1846 324.1773
235 325.1849 324.1776
236 325.1854 324.1781
237 325.1866 324.1793
238 325.2019 324.1946
239 325.2020 324.1947
240 325.2021 324.1948
241 325.2216 324.2143
242 325.2216 324.2143
243 325.2218 324.2145
244 325.3499 324.3426
245 326.1479 325.1406
246 327.1329 326.1256
247 327.1333 326.1260
248 327.1637 326.1564
249 327.1990 326.1917
250 327.2015 326.1942
251 327.2015 326.1942
252 327.2017 326.1944
253 327.2018 326.1945
254 327.2018 326.1945
255 327.2177 326.2104
256 327.2180 326.2107
257 327.2184 326.2111
258 328.1068 327.0995
259 329.1779 328.1706
260 329.1940 328.1867
261 329.2168 328.2095
262 329.2170 328.2097
263 331.1899 330.1826
264 331.1908 330.1835
265 331.1908 330.1835
266 331.1908 330.1835
267 331.1908 330.1835
268 331.1909 330.1836
269 331.1909 330.1836
270 331.1909 330.1836
271 331.1911 330.1838
272 331.2108 330.2035
273 331.2108 330.2035
274 331.2112 330.2039
275 331.2113 330.2040
276 333.0684 332.0611
277 333.2054 332.1981
278 333.2064 332.1991
279 333.2064 332.1991
280 333.2067 332.1994
281 333.2067 332.1994
282 333.2068 332.1995
283 333.2069 332.1996
284 333.2072 332.1999
285 333.2074 332.2001
286 333.2077 332.2004
287 333.2077 332.2004
288 333.2077 332.2004
289 333.2241 332.2168
290 333.2250 332.2177
291 333.2271 332.2198
292 333.2272 332.2199
293 333.2274 332.2201
294 333.2278 332.2205
295 335.1862 334.1789
296 335.1864 334.1791
297 335.1867 334.1794
298 335.1867 334.1794
299 335.1869 334.1796
300 335.1884 334.1811
301 335.2212 334.2139
302 335.2221 334.2148
303 335.2223 334.2150
304 335.2224 334.2151
305 335.2224 334.2151
306 335.2227 334.2154
307 335.2229 334.2156
308 335.2231 334.2158
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