MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
109 309.1891 308.1818
110 309.1902 308.1829
111 309.1904 308.1831
112 309.2057 308.1984
113 309.2069 308.1996
114 309.2069 308.1996
115 309.2072 308.1999
116 309.2074 308.2001
117 309.2077 308.2004
118 309.2081 308.2008
119 309.2082 308.2009
120 309.2085 308.2012
121 309.2086 308.2013
122 310.1906 309.1833
123 310.1920 309.1847
124 311.1675 310.1602
125 311.1676 310.1603
126 311.1677 310.1604
127 311.1677 310.1604
128 311.1680 310.1607
129 311.1683 310.1610
130 311.1687 310.1614
131 311.1692 310.1619
132 311.1855 310.1782
133 311.1862 310.1789
134 311.2061 310.1988
135 311.2063 310.1990
136 311.2065 310.1992
137 311.2065 310.1992
138 311.2065 310.1992
139 311.2232 310.2159
140 311.2238 310.2165
141 311.2239 310.2166
142 311.2244 310.2171
143 311.2248 310.2175
144 312.1328 311.1255
145 312.1328 311.1255
146 312.1331 311.1258
147 312.2086 311.2013
148 313.1224 312.1151
149 313.1836 312.1763
150 313.1842 312.1769
151 313.1845 312.1772
152 313.1845 312.1772
153 313.1861 312.1788
154 313.1864 312.1791
155 313.1870 312.1797
156 313.2209 312.2136
157 313.2217 312.2144
158 313.2220 312.2147
159 313.2386 312.2313
160 313.2400 312.2327
161 313.2400 312.2327
162 313.2401 312.2328
163 315.2008 314.1935
164 315.2014 314.1941
165 316.1912 315.1839
166 317.2108 316.2035
167 319.1894 318.1821
168 319.1900 318.1827
169 319.1902 318.1829
170 319.1904 318.1831
171 319.1904 318.1831
172 319.1906 318.1833
173 319.1908 318.1835
174 319.1908 318.1835
175 319.1910 318.1837
176 319.2256 318.2183
177 319.2267 318.2194
178 319.2269 318.2196
179 319.2271 318.2198
180 319.2272 318.2199
181 319.2272 318.2199
182 319.2274 318.2201
183 321.1695 320.1622
184 321.1698 320.1625
185 321.1699 320.1626
186 321.1701 320.1628
187 321.1704 320.1631
188 321.1706 320.1633
189 321.1708 320.1635
190 321.1708 320.1635
191 321.1713 320.1640
192 321.2049 320.1976
193 321.2060 320.1987
194 321.2061 320.1988
195 321.2063 320.1990
196 321.2063 320.1990
197 321.2418 320.2345
198 321.2427 320.2354
199 321.2428 320.2355
200 321.2428 320.2355
201 321.2430 320.2357
202 321.2608 320.2535
203 323.1678 322.1605
204 323.1681 322.1608
205 323.1681 322.1608
206 323.1689 322.1616
207 323.1693 322.1620
208 323.1698 322.1625
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