MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
9 301.1439 300.1366
10 301.1441 300.1368
11 301.1441 300.1368
12 301.1443 300.1370
13 301.2218 300.2145
14 301.8909 300.8836
15 303.8863 302.8790
16 303.8863 302.8790
17 303.8863 302.8790
18 303.8865 302.8792
19 303.8867 302.8794
20 303.8869 302.8796
21 303.8871 302.8798
22 303.8871 302.8798
23 305.0121 304.0048
24 305.0125 304.0052
25 305.1740 304.1667
26 305.1748 304.1675
27 305.1749 304.1676
28 305.1750 304.1677
29 305.1750 304.1677
30 305.1751 304.1678
31 305.1753 304.1680
32 305.1754 304.1681
33 305.1755 304.1682
34 305.1756 304.1683
35 305.1758 304.1685
36 305.1760 304.1687
37 305.1761 304.1688
38 305.1948 304.1875
39 305.2103 304.2030
40 305.2111 304.2038
41 305.2114 304.2041
42 307.1534 306.1461
43 307.1543 306.1470
44 307.1547 306.1474
45 307.1547 306.1474
46 307.1549 306.1476
47 307.1898 306.1825
48 307.1908 306.1835
49 307.1908 306.1835
50 307.1908 306.1835
51 307.1909 306.1836
52 307.1912 306.1839
53 307.1914 306.1841
54 307.1914 306.1841
55 307.1918 306.1845
56 307.1919 306.1846
57 307.1921 306.1848
58 307.1921 306.1848
59 307.1922 306.1849
60 307.2096 306.2023
61 307.2104 306.2031
62 308.0894 307.0821
63 308.0895 307.0822
64 308.0897 307.0824
65 308.0898 307.0825
66 308.0898 307.0825
67 308.0899 307.0826
68 308.0901 307.0828
69 308.0903 307.0830
70 308.0904 307.0831
71 308.0905 307.0832
72 308.0905 307.0832
73 308.0908 307.0835
74 308.0909 307.0836
75 308.0911 307.0838
76 308.0911 307.0838
77 308.0919 307.0846
78 308.0919 307.0846
79 308.0921 307.0848
80 308.0921 307.0848
81 308.0922 307.0849
82 308.0923 307.0850
83 308.0924 307.0851
84 308.0925 307.0852
85 308.0925 307.0852
86 308.0927 307.0854
87 308.0927 307.0854
88 308.0927 307.0854
89 308.0927 307.0854
90 308.0928 307.0855
91 308.0928 307.0855
92 308.0928 307.0855
93 308.0930 307.0857
94 308.0930 307.0857
95 308.0930 307.0857
96 308.0931 307.0858
97 309.1528 308.1455
98 309.1530 308.1457
99 309.1534 308.1461
100 309.1694 308.1621
101 309.1702 308.1629
102 309.1703 308.1630
103 309.1704 308.1631
104 309.1707 308.1634
105 309.1708 308.1635
106 309.1874 308.1801
107 309.1887 308.1814
108 309.1888 308.1815
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