MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 3 |
| MS data file | : | PRT1245_Other_P1_B11.mgf |
| Database | : | S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues) |
| Timestamp | : | 24 Jun 2025 at 21:21:58 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,722 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 2558 |
643.8303 | 1285.6460 | 1285.6374 | 6.70 | 1 | 1 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
INGNGKNNIDAR + Deamidated (NQ) |
| 2472 |
600.2755 | 1198.5364 | 1198.5466 | -8.43 | 1 | 1 | 3.6 | 1Score > 19 indicates identity |
EPKNENDEPK |
| 3097 |
564.6341 | 1690.8805 | 1690.8566 | 14.1 | 0 | 1 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
DDFAYIIELIQSHK |
| 2911 |
523.9290 | 1568.7652 | 1568.7617 | 2.23 | 0 | 1 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
MNNTSITGPQVLHR + 2 Deamidated (NQ) |
| 2899 |
781.8989 | 1561.7832 | 1561.8100 | -17.1 | 1 | 1 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
DINDININFAAKSK |
| 3117 |
855.4347 | 1708.8548 | 1708.8492 | 3.30 | 1 | 1 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
NSIRSIINHPEDSAR + Deamidated (NQ) |
| 3163 |
581.6651 | 1741.9735 | 1741.9403 | 19.1 | 1 | 1 | 2.4 | 1Score > 17 indicates identity |
QIFLPNKQHFLDIK + 2 Deamidated (NQ) |
| 3411 |
972.4617 | 1942.9088 | 1942.9266 | -9.12 | 1 | 1 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
KSTSSSINSISNMGTSAVR + Deamidated (NQ); Oxidation (M) |
| 2632 |
677.3454 | 1352.6762 | 1352.6572 | 14.1 | 1 | 1 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
KLENDYTSLNR + Deamidated (NQ) |
| 2173 |
560.7740 | 1119.5334 | 1119.5342 | -0.69 | 1 | 1 | 0.91 | 1Score > 21 indicates identity Score > 13 indicates homology |
NAENGQMTKK |
| 2260 |
571.7637 | 1141.5128 | 1141.5251 | -10.7 | 0 | 1 | 4.2 | 1Score > 19 indicates identity |
TASAPNSGNPPK + 2 Deamidated (NQ) |
| 1135 |
371.1809 | 740.3472 | 740.3453 | 2.64 | 0 | 1 | 2.4 | 1Score > 17 indicates identity |
HNNDLK + Deamidated (NQ) |
| 3856 |
1085.5380 | 2169.0614 | 2169.0841 | -10.4 | 1 | 1 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
SAEYSLKEIQIEELQDFK |
| 4364 |
963.1185 | 2886.3337 | 2886.3878 | -18.7 | 0 | 1 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
NITGISNSELLSEMNNDISMFGILLK + 2 Deamidated (NQ); 2 Oxidation (M) |
| 1227 |
800.3809 | 799.3736 | 799.3864 | -16.0 | 0 | 1 | 2.1 | 1Score > 16 indicates identity |
YNYAIR + Deamidated (NQ) |
| 1580 |
950.4611 | 949.4538 | 949.4716 | -18.7 | 1 | 1 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
LSDGSSEKK |
| 3853 |
1083.9905 | 2165.9664 | 2165.9600 | 2.97 | 0 | 1 | 1 | 1Score > 20 indicates identity Score > 13 indicates homology |
GQLDLINDGEGSLSNFADNGK + 3 Deamidated (NQ) |
| 4667 |
902.0750 | 4505.3386 | 4505.2846 | 12.0 | 1 | 1 | 1.9 | 1Score > 16 indicates identity |
TILDIVNIQNELSNHLTVQSQNINLMLNNQDDIELNIKK + 4 Deamidated (NQ) |
| 1798 |
1021.5449 | 1020.5376 | 1020.5240 | 13.3 | 0 | 1 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
ETALDFIGR |
| 2647 |
687.3440 | 1372.6734 | 1372.6697 | 2.75 | 0 | 1 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
LSLDSFFEMIR + Oxidation (M) |
| 2869 |
513.5782 | 1537.7128 | 1537.6871 | 16.7 | 1 | 1 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
YEKMFYNQAATR + Deamidated (NQ); Oxidation (M) |
| 3148 |
868.4635 | 1734.9124 | 1734.9021 | 5.96 | 0 | 1 | 0.97 | 1Score > 21 indicates identity Score > 13 indicates homology |
ILFQYWLFYNSLK + Deamidated (NQ) |
| 1581 |
950.4705 | 949.4632 | 949.4756 | -13.1 | 0 | 1 | 0.97 | 1Score > 22 indicates identity Score > 13 indicates homology |
NNVVINFK + 3 Deamidated (NQ) |
| 3370 |
952.4506 | 1902.8866 | 1902.9058 | -10.1 | 1 | 1 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
KNGSQEQELGDIVDEIK + 2 Deamidated (NQ) |
| 4604 |
1184.8998 | 3551.6776 | 3551.7429 | -18.4 | 0 | 1 | 1 | 1Score > 20 indicates identity Score > 13 indicates homology |
IAYTFGWEGPALTVDTACSSSLVGIHLAMQALR + Deamidated (NQ); Oxidation (M) |
| 2905 |
781.9234 | 1561.8322 | 1561.8028 | 18.9 | 1 | 1 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
GILNYYKLATNYK + 2 Deamidated (NQ) |
| 3491 |
998.4747 | 1994.9348 | 1994.9619 | -13.6 | 0 | 1 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
GIVDIYSNDVMIIDNNGK + Oxidation (M) |
| 3499 |
668.7001 | 2003.0785 | 2003.0840 | -2.76 | 1 | 1 | 4.1 | 1Score > 19 indicates identity |
FQAERGLDVLVLLTSWR + Deamidated (NQ) |
| 2237 |
568.8420 | 1135.6694 | 1135.6601 | 8.22 | 1 | 1 | 1.1 | 1Score > 14 indicates identity |
KTQVYTGIVK |
| 4274 |
917.1353 | 2748.3841 | 2748.3316 | 19.1 | 1 | 1 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
NAAWLVFANKQDLPEAMSAAEITEK + 2 Deamidated (NQ) |
| 1063 |
339.6647 | 677.3148 | 677.3166 | -2.66 | 0 | 1 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
MSTPAR + Oxidation (M) |
| 1142 |
743.4055 | 742.3982 | 742.3974 | 1.17 | 0 | 1 | 2.9 | 1Score > 18 indicates identity |
QGGSAPVK |
| 2069 |
547.7656 | 1093.5166 | 1093.5186 | -1.77 | 1 | 1 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
SQVSQNRMK + Deamidated (NQ); Oxidation (M) |
| 3618 |
689.3560 | 2065.0462 | 2065.0102 | 17.4 | 1 | 1 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
AVYENNETGVIKNLELQK + 4 Deamidated (NQ) |
| 4107 |
802.0816 | 2403.2230 | 2403.2091 | 5.79 | 0 | 1 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
VNIALAELLTVSTDDTDMPLEK + Oxidation (M) |
| 4650 |
821.2094 | 4101.0106 | 4101.0214 | -2.62 | 0 | 0 | 4 | 1Score > 19 indicates identity |
IMLVFVIIDGITSNESILDMSQVNYIPDQYGNPQLK + 2 Deamidated (NQ); 2 Oxidation (M) |
| 4077 |
594.3049 | 2373.1905 | 2373.2151 | -10.4 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
FYRVVNMSFGGDTNLILAALR + Deamidated (NQ); Oxidation (M) |
| 1853 |
518.7766 | 1035.5386 | 1035.5284 | 9.91 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
LGHPCPLSR |
| 2532 |
631.3194 | 1260.6242 | 1260.6197 | 3.59 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
GKQIESEGGNLK + 2 Deamidated (NQ) |
| 1890 |
524.7632 | 1047.5118 | 1047.5026 | 8.86 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
GPFNWTVEV |
| 2524 |
628.3115 | 1254.6084 | 1254.6278 | -15.4 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
YMEQIKGTGTK |
| 1628 |
967.4886 | 966.4813 | 966.4844 | -3.22 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
KGIQMFDK + Deamidated (NQ) |
| 3162 |
581.6646 | 1741.9720 | 1741.9978 | -14.8 | 0 | 0 | 2.6 | 1Score > 17 indicates identity |
FAASIPSVVNVLDAVIK |
| 1462 |
911.4603 | 910.4530 | 910.4621 | -9.93 | 1 | 0 | 4.5 | 1Score > 20 indicates identity |
KHATPSDR |
| 2975 |
534.9495 | 1601.8267 | 1601.8382 | -7.17 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
STVPIIIRCCIDR |
| 2391 |
585.7833 | 1169.5520 | 1169.5313 | 17.8 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
ADPTNGPAENGK |
| 2995 |
810.4095 | 1618.8044 | 1618.7872 | 10.6 | 1 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
EQQQLGMLDDKVAK + Deamidated (NQ); Oxidation (M) |
| 1217 |
398.2102 | 794.4058 | 794.4174 | -14.6 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
VSTTYPK |
| 4033 |
777.3722 | 2329.0948 | 2329.1260 | -13.4 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
MSDNLLSLENPVVPSHYELR + Deamidated (NQ); Oxidation (M) |
| 1014 |
315.6646 | 629.3146 | 629.3133 | 2.18 | 0 | 0 | 1.6 | 1Score > 15 indicates identity |
TAQPGR + Deamidated (NQ) |
| 2901 |
781.9012 | 1561.7878 | 1561.7592 | 18.3 | 0 | 0 | 0.98 | 1Score > 23 indicates identity Score > 13 indicates homology |
IICPSVESISNGMR |
| 4024 |
775.4034 | 2323.1884 | 2323.1478 | 17.5 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
TIMNIDATTEGENLHVLSPPR + Oxidation (M) |
| 1621 |
483.7363 | 965.4580 | 965.4753 | -17.8 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
IATRGAFAC |
| 4100 |
600.5532 | 2398.1837 | 2398.1917 | -3.34 | 1 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
QPGHTDWAVIQLGRESSFIEK + Deamidated (NQ) |
| 2090 |
550.2736 | 1098.5326 | 1098.5458 | -12.0 | 0 | 0 | 1 | 1Score > 20 indicates identity Score > 13 indicates homology |
TYYLQGAQR |
| 1589 |
953.4348 | 952.4275 | 952.4362 | -9.14 | 1 | 0 | 3.8 | 1Score > 19 indicates identity |
DAYRGQSR + Deamidated (NQ) |
| 4063 |
1183.5724 | 2365.1302 | 2365.1471 | -7.11 | 1 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
SMNLLQELSPRLFEAANASEK + 2 Deamidated (NQ); Oxidation (M) |
| 2691 |
716.3846 | 1430.7546 | 1430.7518 | 2.02 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
IGAWASRQSDVIK + Deamidated (NQ) |
| 2800 |
749.3730 | 1496.7314 | 1496.7147 | 11.2 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
LDSEFQPLSSAFR + Deamidated (NQ) |
| 2812 |
752.8723 | 1503.7300 | 1503.7425 | -8.31 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
SPTGIVLMNMGGPSK + Oxidation (M) |
| 1089 |
349.1982 | 696.3818 | 696.3919 | -14.4 | 0 | 0 | 1.6 | 1Score > 15 indicates identity |
SVNHLK |
| 2336 |
579.7607 | 1157.5068 | 1157.5274 | -17.8 | 0 | 0 | 3.7 | 1Score > 18 indicates identity |
GNYMLVSQTK + 2 Deamidated (NQ); Oxidation (M) |
| 4413 |
992.8385 | 2975.4937 | 2975.5202 | -8.90 | 1 | 0 | 0.96 | 1Score > 21 indicates identity Score > 13 indicates homology |
KNTTYVATLIYEFIILNDASMTPDVK + Oxidation (M) |
| 4709 |
1124.8068 | 5618.9976 | 5618.9967 | 0.16 | 1 | 0 | 0.92 | 1Score > 13 indicates identity |
AMVLHQVCNAMIMVLVISMAISFAVRDWITGGVISFVIAVNVLIGLVQEYK + Deamidated (NQ) |
| 1230 |
400.7171 | 799.4196 | 799.4188 | 1.05 | 0 | 0 | 3.6 | 1Score > 18 indicates identity |
QKPDQGK |
| 2658 |
695.3385 | 1388.6624 | 1388.6831 | -14.8 | 0 | 0 | 0.96 | 1Score > 21 indicates identity Score > 13 indicates homology |
NGMVVGLGAGQQSR + Oxidation (M) |
| 2900 |
521.6019 | 1561.7839 | 1561.7997 | -10.1 | 0 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
VLQTMSFLMNHLI + Oxidation (M) |
| 2952 |
794.9087 | 1587.8028 | 1587.8256 | -14.3 | 1 | 0 | 1 | 1Score > 24 indicates identity Score > 13 indicates homology |
AAQNPLADIQVYKR + 2 Deamidated (NQ) |
| 3328 |
622.6384 | 1864.8934 | 1864.8625 | 16.6 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
EEAGAANEQMPYNKAVK + Oxidation (M) |
| 4676 |
940.6689 | 4698.3081 | 4698.2518 | 12.0 | 1 | 0 | 3.8 | 1Score > 19 indicates identity |
FQKPYNPLAMDSYALGIMLITMINNIIPFIDSCNTDARFR + 3 Deamidated (NQ); 2 Oxidation (M) |
| 2305 |
1152.5498 | 1151.5425 | 1151.5346 | 6.86 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
ITDDNEIGFK + Deamidated (NQ) |
| 3100 |
847.3828 | 1692.7510 | 1692.7559 | -2.89 | 1 | 0 | 1 | 1Score > 20 indicates identity Score > 13 indicates homology |
ETSNKQTACPICQR + Deamidated (NQ) |
| 4339 |
943.8542 | 2828.5408 | 2828.5840 | -15.3 | 1 | 0 | 1.7 | 1Score > 15 indicates identity |
TLIFSRFSFNLVLLAVVDYQIITR + Deamidated (NQ) |
| 2753 |
732.3587 | 1462.7028 | 1462.6973 | 3.77 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
SLNTNSPISELCK + Deamidated (NQ) |
| 2499 |
610.2943 | 1218.5740 | 1218.5564 | 14.5 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
GKSANFCGNHK |
| 3342 |
469.9762 | 1875.8757 | 1875.9115 | -19.1 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
REPFFHGTSNTDQLVK + Deamidated (NQ) |
| 2923 |
788.3784 | 1574.7422 | 1574.7576 | -9.77 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
SGVQTAYIDYSSKR + Deamidated (NQ) |
| 4285 |
922.1370 | 2763.3892 | 2763.4402 | -18.5 | 1 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
SLNELSLSDILIAADVEGGLHAVDRR + Deamidated (NQ) |
| 2619 |
672.8279 | 1343.6412 | 1343.6510 | -7.25 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
DGEWLYHISPK |
| 2935 |
789.9206 | 1577.8266 | 1577.8487 | -14.0 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
LKFASNLMVINPSK + Deamidated (NQ); Oxidation (M) |
| 3147 |
868.4271 | 1734.8396 | 1734.8617 | -12.7 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
ERNYIVAFIFGNYK + 2 Deamidated (NQ) |
| 3179 |
584.6035 | 1750.7887 | 1750.8009 | -7.00 | 1 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
QPDDIINNDEPSHKK + 2 Deamidated (NQ) |
| 3655 |
1039.5046 | 2076.9946 | 2077.0361 | -20.0 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
SLDELCGVQLTSTLRQQK + 2 Deamidated (NQ) |
| 3905 |
739.3282 | 2214.9628 | 2214.9742 | -5.14 | 1 | 0 | 3.8 | 1Score > 18 indicates identity |
VQLCTLMNIKSGGCSEDCK + Oxidation (M) |
| 4529 |
1079.8875 | 3236.6407 | 3236.6324 | 2.57 | 1 | 0 | 1 | 1Score > 20 indicates identity Score > 13 indicates homology |
QSASVTLWQVIGNSVMAFILGSMFYKVMK + 2 Deamidated (NQ) |
| 3070 |
836.9415 | 1671.8684 | 1671.8679 | 0.32 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
SIPPVTSSIQAQQSTK + Deamidated (NQ) |
| 2541 |
636.3044 | 1270.5942 | 1270.5789 | 12.1 | 0 | 0 | 1 | 1Score > 21 indicates identity Score > 13 indicates homology |
NGQASAPRPNQK + 4 Deamidated (NQ) |
| 3426 |
976.9856 | 1951.9566 | 1951.9350 | 11.1 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
EEAAPKPPSSAVFSMFGGK + Oxidation (M) |
| 3589 |
1029.4906 | 2056.9666 | 2056.9371 | 14.4 | 1 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
SMTPFSAGNTSSQNKLENK + Deamidated (NQ); Oxidation (M) |
| 2898 |
521.5998 | 1561.7776 | 1561.7997 | -14.1 | 0 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
VLQTMSFLMNHLI + Oxidation (M) |
| 1957 |
1064.5415 | 1063.5342 | 1063.5410 | -6.40 | 1 | 0 | 1 | 1Score > 23 indicates identity Score > 13 indicates homology |
RTAQFEANK |
| 2482 |
603.7983 | 1205.5820 | 1205.5962 | -11.7 | 0 | 0 | 1 | 1Score > 22 indicates identity Score > 13 indicates homology |
IGIETNMLGDK + Oxidation (M) |
| 1 |
300.0932 | 299.0859 | |||||||
| 2 |
300.2087 | 299.2014 | |||||||
| 3 |
301.1169 | 300.1096 | |||||||
| 4 |
301.1435 | 300.1362 | |||||||
| 5 |
301.1435 | 300.1362 | |||||||
| 6 |
301.1435 | 300.1362 | |||||||
| 7 |
301.1436 | 300.1363 | |||||||
| 8 |
301.1438 | 300.1365 | |||||||
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