MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4501–4600 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4566 1129.4652 3385.3738
4567 1132.8811 3395.6215
4568 1135.5046 3403.4920
4569 1135.8357 3404.4853
4570 1140.5374 3418.5904
4571 1141.5580 3421.6522
4572 1141.5597 3421.6573
4573 856.4227 3421.6617
4574 1141.8783 3422.6131
4575 1142.5791 3424.7155
4576 857.4268 3425.6781
4577 857.4365 3425.7169
4578 858.1824 3428.7005
4579 858.4384 3429.7245
4580 1144.2682 3429.7828
4581 1144.5923 3430.7551
4582 1144.6050 3430.7932
4583 1145.5369 3433.5889
4584 861.4448 3441.7501
4585 865.4344 3457.7085
4586 865.4439 3457.7465
4587 1154.8828 3461.6266
4588 1156.5646 3466.6720
4589 1158.7957 3473.3653
4590 1161.5758 3481.7056
4591 1161.5929 3481.7569
4592 1166.2250 3495.6532
4593 1170.5651 3508.6735
4594 1171.5056 3511.4950
4595 1171.5225 3511.5457
4596 1175.5676 3523.6810
4597 1178.5750 3532.7032
4598 1179.5719 3535.6939
4601 888.4349 3549.7105
4602 1184.5686 3550.6840
4603 1184.5703 3550.6891
4605 1185.5618 3553.6636
4606 1189.2368 3564.6886
4607 1189.5737 3565.6993
4608 1193.5881 3577.7425
4609 895.4446 3577.7493
4610 906.4922 3621.9397
4611 909.4426 3633.7413
4612 911.9887 3643.9257
4613 914.4561 3653.7953
4614 915.9769 3659.8785
4615 916.4485 3661.7649
4617 733.3822 3661.8746
4618 736.4030 3676.9786
4619 920.4528 3677.7821
4620 920.4620 3677.8189
4621 920.7040 3678.7869
4622 923.6951 3690.7513
4623 924.4578 3693.8021
4624 926.4464 3701.7565
4626 930.4431 3717.7433
4627 934.0526 3732.1813
4628 934.4738 3733.8661
4629 938.7648 3751.0301
4630 973.2480 3888.9629
4631 973.5009 3889.9745
4632 986.7211 3942.8553
4633 990.7143 3958.8281
4634 1012.7724 4047.0605
4635 1013.0210 4048.0549
4636 1013.0227 4048.0617
4637 1013.0227 4048.0617
4638 1013.0233 4048.0641
4639 1013.0239 4048.0665
4640 1013.0262 4048.0757
4641 1013.0265 4048.0769
4642 1013.0317 4048.0977
4643 1013.0333 4048.1041
4644 1013.0334 4048.1045
4645 1016.5282 4062.0837
4646 1016.7709 4063.0545
4647 1016.7723 4063.0601
4648 1016.7763 4063.0761
4649 1016.7763 4063.0761
4651 1044.0521 4172.1793
4652 1051.2871 4201.1193
4654 1057.7642 4227.0277
4655 1072.7678 4287.0421
4656 1073.2507 4288.9737
4657 1079.8079 4315.2025
4658 1084.0706 4332.2533
4659 1086.2565 4340.9969
4660 1095.3529 4377.3825
4661 1100.5070 4397.9989
4662 1100.7554 4398.9925
4663 1104.0551 4412.1913
4664 1122.5479 4486.1625
4665 1127.0936 4504.3453
4666 902.0746 4505.3366
4669 1138.3463 4549.3561
4670 913.2802 4561.3646
4671 913.4814 4562.3706
4672 916.6786 4578.3566
4673 929.0655 4640.2911
4674 929.4653 4642.2901
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