MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4401–4500 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4458 1010.4441 3028.3105
4459 1010.4554 3028.3444
4460 1010.5480 3028.6222
4461 1010.5531 3028.6375
4462 758.3613 3029.4161
4463 1011.1656 3030.4750
4464 1012.5319 3034.5739
4465 1014.4999 3040.4779
4466 761.1302 3040.4917
4467 1014.5077 3040.5013
4468 1015.5051 3043.4935
4469 1016.4908 3046.4506
4470 1017.8727 3050.5963
4471 1018.8442 3053.5108
4472 764.3856 3053.5133
4473 1019.5118 3055.5136
4474 1019.8141 3056.4205
4475 1020.4886 3058.4440
4476 1024.1752 3069.5038
4477 1024.4592 3070.3558
4478 1024.5062 3070.4968
4479 1025.4772 3073.4098
4480 1025.8214 3074.4424
4481 1028.1802 3081.5188
4482 1028.5092 3082.5058
4483 1028.5208 3082.5406
4484 1029.4921 3085.4545
4485 1029.5155 3085.5247
4486 776.1340 3100.5069
4487 1034.8268 3101.4586
4488 1034.8406 3101.5000
4489 1035.4882 3103.4428
4490 1038.9095 3113.7067
4492 1039.8409 3116.5009
4493 780.3853 3117.5121
4494 1040.8424 3119.5054
4495 782.4061 3125.5953
4496 1044.5093 3130.5061
4497 1045.5065 3133.4977
4499 1049.1814 3144.5224
4500 787.8975 3147.5609
4501 1053.5177 3157.5313
4502 790.3936 3157.5453
4504 1054.1865 3159.5377
4505 792.1721 3164.6593
4506 792.4213 3165.6561
4507 792.4237 3165.6657
4509 792.6729 3166.6625
4510 1058.8505 3173.5297
4511 1059.5153 3175.5241
4512 1059.8468 3176.5186
4513 1062.4781 3184.4125
4514 1062.7942 3185.3608
4515 1065.1743 3192.5011
4516 1065.5068 3193.4986
4517 1065.5436 3193.6090
4518 1066.2122 3195.6148
4519 1066.5186 3196.5340
4520 1067.4592 3199.3558
4521 1067.7976 3200.3710
4522 1070.8789 3209.6149
4523 1070.8854 3209.6344
4524 1071.8472 3212.5198
4525 1071.8485 3212.5237
4526 804.3817 3213.4977
4527 1072.5229 3214.5469
4528 808.3864 3229.5165
4530 1082.1777 3243.5113
4531 1082.5099 3244.5079
4532 1085.8973 3254.6701
4534 1088.5258 3262.5556
4535 1090.5776 3268.7110
4536 1091.1870 3270.5392
4537 819.1850 3272.7109
4538 1094.5480 3280.6222
4540 822.3911 3285.5353
4541 1096.2214 3285.6424
4542 1096.5204 3286.5394
4543 1097.4761 3289.4065
4544 1097.8217 3290.4433
4545 1100.2274 3297.6604
4547 1102.5677 3304.6813
4548 1108.5350 3322.5832
4549 1110.5464 3328.6174
4550 1111.4896 3331.4470
4551 1111.5023 3331.4851
4552 1111.5237 3331.5493
4553 1111.5348 3331.5826
4554 1116.4868 3346.4386
4555 1116.8253 3347.4541
4556 1117.1876 3348.5410
4557 1117.5439 3349.6099
4558 838.7153 3350.8321
4559 1122.8746 3365.6020
4560 843.1779 3368.6825
4561 1123.9030 3368.6872
4562 1124.1444 3369.4114
4563 1124.1493 3369.4261
4564 1129.1353 3384.3841
4565 1129.2317 3384.6733
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