MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4301–4400 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4345 947.4769 2839.4089
4346 949.1181 2844.3325
4347 949.4518 2845.3336
4348 950.7603 2849.2591
4349 951.4650 2851.3732
4350 714.3624 2853.4205
4351 952.4781 2854.4125
4353 953.4472 2857.3198
4354 953.7985 2858.3737
4355 955.4750 2863.4032
4356 956.4896 2866.4470
4357 957.1268 2868.3586
4358 957.4681 2869.3825
4359 961.8165 2882.4277
4360 961.8206 2882.4400
4361 962.7933 2885.3581
4362 962.8119 2885.4139
4363 962.8705 2885.5897
4365 963.1965 2886.5677
4367 967.4772 2899.4098
4369 971.1556 2910.4450
4370 729.6439 2914.5465
4371 975.4666 2923.3780
4372 975.4684 2923.3834
4373 975.4941 2923.4605
4374 980.4599 2938.3579
4375 980.7957 2939.3653
4376 981.1352 2940.3838
4377 981.1612 2940.4618
4378 981.4984 2941.4734
4379 982.4835 2944.4287
4380 982.5188 2944.5346
4383 983.4599 2947.3579
4384 983.4833 2947.4281
4385 984.4885 2950.4437
4386 984.8265 2951.4577
4387 738.8757 2951.4737
4388 985.4850 2953.4332
4389 986.4662 2956.3768
4390 986.4665 2956.3777
4391 740.1194 2956.4485
4392 986.4929 2956.4569
4393 740.1469 2956.5585
4394 740.1486 2956.5653
4395 986.8005 2957.3797
4396 988.4501 2962.3285
4397 988.4507 2962.3303
4398 988.4647 2962.3723
4399 989.8312 2966.4718
4400 989.8406 2966.5000
4401 742.8739 2967.4665
4402 742.8754 2967.4725
4405 990.4911 2968.4515
4406 990.5248 2968.5526
4407 743.9021 2971.5793
4408 744.1194 2972.4485
4409 991.8235 2972.4487
4412 992.8376 2975.4910
4415 993.4459 2977.3159
4416 993.4559 2977.3459
4417 993.7817 2978.3233
4418 994.5109 2980.5109
4419 994.8194 2981.4364
4420 995.1581 2982.4525
4421 995.1631 2982.4675
4422 746.6255 2982.4729
4423 995.4492 2983.3258
4424 995.4780 2983.4122
4425 995.4876 2983.4410
4426 995.4929 2983.4569
4427 746.8737 2983.4657
4428 995.4980 2983.4722
4429 748.3696 2989.4493
4430 998.4322 2992.2748
4431 998.7698 2993.2876
4432 998.7781 2993.3125
4433 749.8777 2995.4817
4434 750.1315 2996.4969
4435 999.8401 2996.4985
4436 1000.1647 2997.4723
4437 1000.4880 2998.4422
4438 1000.4987 2998.4743
4439 1000.8192 2999.4358
4440 1003.8398 3008.4976
4441 1004.1016 3009.2830
4443 754.1296 3012.4893
4444 754.1530 3012.5829
4445 1005.8281 3014.4625
4446 1005.8293 3014.4661
4447 1005.8821 3014.6245
4448 1006.2278 3015.6616
4449 1006.4986 3016.4740
4450 1006.5720 3016.6942
4451 1008.2121 3021.6145
4452 757.1279 3024.4825
4453 1009.1688 3024.4846
4454 1009.1718 3024.4936
4455 757.3754 3025.4725
4456 1010.1194 3027.3364
4457 1010.2118 3027.6136
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