MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4201–4300 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4237 880.4427 2638.3063
4238 880.4688 2638.3846
4239 660.8682 2639.4437
4240 660.8703 2639.4521
4241 881.7329 2642.1769
4242 883.4142 2647.2208
4243 883.4401 2647.2985
4244 883.4737 2647.3993
4245 883.4772 2647.4098
4246 883.8076 2648.4010
4247 886.0987 2655.2743
4248 887.0546 2658.1420
4249 888.4321 2662.2745
4250 888.8084 2663.4034
4251 534.6874 2668.4006
4252 891.4296 2671.2670
4253 891.4348 2671.2826
4254 895.4457 2683.3153
4255 896.4534 2686.3384
4256 896.4663 2686.3771
4257 898.0766 2691.2080
4258 898.0804 2691.2194
4259 898.4498 2692.3276
4260 898.8433 2693.5081
4261 899.4218 2695.2436
4262 901.0769 2700.2089
4263 901.4365 2701.2877
4265 904.7543 2711.2411
4266 905.4404 2713.2994
4267 909.1802 2724.5188
4268 683.0506 2728.1733
4269 912.0906 2733.2500
4270 912.4627 2734.3663
4272 913.4606 2737.3600
4273 915.4436 2743.3090
4275 917.8425 2750.5057
4276 917.8429 2750.5069
4277 688.6341 2750.5073
4278 688.6343 2750.5081
4279 917.8481 2750.5225
4280 918.1764 2751.5074
4281 918.4274 2752.2604
4282 689.8432 2755.3437
4283 920.4510 2758.3312
4284 921.0986 2760.2740
4287 922.4767 2764.4083
4288 922.4802 2764.4188
4289 922.4868 2764.4386
4290 922.7442 2765.2108
4291 922.8256 2765.4550
4292 922.8359 2765.4859
4293 924.1133 2769.3181
4294 925.1652 2772.4738
4295 925.5167 2773.5283
4296 925.8441 2774.5105
4297 926.1718 2775.4936
4298 926.4375 2776.2907
4299 926.4390 2776.2952
4300 927.8289 2780.4649
4301 927.9933 2780.9581
4302 928.1194 2781.3364
4303 928.6671 2782.9795
4304 929.1323 2784.3751
4305 929.5048 2785.4926
4306 697.8709 2787.4545
4307 930.3300 2787.9682
4308 930.4604 2788.3594
4309 558.6990 2788.4586
4310 698.3715 2789.4569
4311 930.8270 2789.4592
4312 931.1863 2790.5371
4313 931.4555 2791.3447
4315 932.4640 2794.3702
4316 932.7814 2795.3224
4317 932.7839 2795.3299
4318 933.8008 2798.3806
4319 701.3413 2801.3361
4320 701.3425 2801.3409
4322 935.1296 2802.3670
4323 935.1304 2802.3694
4324 935.1323 2802.3751
4325 935.4600 2803.3582
4326 935.4644 2803.3714
4327 936.8197 2807.4373
4328 702.8918 2807.5381
4329 702.8923 2807.5401
4330 937.4384 2809.2934
4331 938.1137 2811.3193
4332 938.4613 2812.3621
4333 938.4714 2812.3924
4334 940.4698 2818.3876
4335 942.1325 2823.3757
4336 943.7741 2828.3005
4337 943.7832 2828.3278
4338 943.7867 2828.3383
4340 945.4260 2833.2562
4341 945.4398 2833.2976
4342 945.8120 2834.4142
4343 946.1853 2835.5341
4344 710.8589 2839.4065
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