MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4131 814.4023 2440.1851
4132 814.4147 2440.2223
4133 814.7010 2441.0812
4134 815.0632 2442.1678
4135 816.7508 2447.2306
4136 817.1357 2448.3853
4137 817.4129 2449.2169
4138 817.4213 2449.2421
4139 819.0392 2454.0958
4140 819.4188 2455.2346
4141 819.4189 2455.2349
4142 819.7314 2456.1724
4143 820.0594 2457.1564
4144 820.7736 2459.2990
4145 821.3957 2461.1653
4146 822.1072 2463.2998
4147 822.7717 2465.2933
4148 823.1224 2466.3454
4149 823.1238 2466.3496
4150 824.3656 2470.0750
4151 824.7485 2471.2237
4152 618.8135 2471.2249
4153 824.7500 2471.2282
4154 619.0682 2472.2437
4155 825.3932 2473.1578
4156 826.7177 2477.1313
4157 827.1350 2478.3832
4158 827.4647 2479.3723
4159 828.4061 2482.1965
4160 828.4168 2482.2286
4161 829.3892 2485.1458
4162 829.3958 2485.1656
4163 829.4185 2485.2337
4165 830.0795 2487.2167
4166 623.0659 2488.2345
4167 830.4235 2488.2487
4168 832.4252 2494.2538
4169 833.0781 2496.2125
4170 834.3723 2500.0951
4171 834.3850 2500.1332
4172 834.4073 2500.2001
4173 834.4156 2500.2250
4174 834.4598 2500.3576
4175 834.7501 2501.2285
4176 835.0712 2502.1918
4177 837.4000 2509.1782
4178 837.4433 2509.3081
4179 837.7305 2510.1697
4180 838.0454 2511.1144
4181 838.4245 2512.2517
4182 838.4261 2512.2565
4183 839.3721 2515.0945
4184 839.7828 2516.3266
4185 630.0900 2516.3309
4186 841.4055 2521.1947
4187 841.4814 2521.4224
4188 842.3852 2524.1338
4189 842.4088 2524.2046
4192 843.0726 2526.1960
4193 843.3920 2527.1542
4194 843.4097 2527.2073
4195 843.7567 2528.2483
4196 843.7576 2528.2510
4197 847.7302 2540.1688
4198 848.4311 2542.2715
4199 849.0869 2544.2389
4200 849.0876 2544.2410
4201 849.3793 2545.1161
4203 851.3877 2551.1413
4204 853.4212 2557.2418
4205 854.7137 2561.1193
4206 856.0623 2565.1651
4208 857.4272 2569.2598
4209 857.4308 2569.2706
4210 858.0601 2571.1585
4211 860.4146 2578.2220
4213 862.4175 2584.2307
4214 862.7219 2585.1439
4215 862.7598 2585.2576
4216 863.3930 2587.1572
4217 863.4019 2587.1839
4218 863.4290 2587.2652
4219 863.4552 2587.3438
4220 864.4954 2590.4644
4221 866.4028 2596.1866
4222 866.4215 2596.2427
4223 866.7360 2597.1862
4224 867.0901 2598.2485
4225 867.3594 2599.0564
4226 869.0865 2604.2377
4227 652.8099 2607.2105
4228 872.4002 2614.1788
4229 872.4106 2614.2100
4230 872.4216 2614.2430
4231 873.7211 2618.1415
4232 874.4228 2620.2466
4233 876.4124 2626.2154
4234 876.4348 2626.2826
4235 876.7432 2627.2078
4236 877.0677 2628.1813
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