MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4013 1157.5658 2313.1170
4014 1157.5671 2313.1196
4015 772.0473 2313.1201
4016 772.3644 2314.0714
4017 772.3724 2314.0954
4018 772.3881 2314.1425
4019 774.0637 2319.1693
4020 774.9811 2321.9215
4021 1162.5695 2323.1244
4022 775.3839 2323.1299
4023 1162.5990 2323.1834
4025 775.4038 2323.1896
4026 775.7148 2324.1226
4027 1163.0687 2324.1228
4028 775.7159 2324.1259
4029 776.0742 2325.2008
4030 776.3520 2326.0342
4031 776.3695 2326.0867
4032 777.3720 2329.0942
4034 1165.5551 2329.0956
4035 1165.5563 2329.0980
4036 777.7131 2330.1175
4037 779.3670 2335.0792
4038 1168.5500 2335.0854
4039 780.1165 2337.3277
4041 780.3959 2338.1659
4042 1170.5938 2339.1730
4043 781.3705 2341.0897
4044 782.4001 2344.1785
4045 782.4059 2344.1959
4046 782.4086 2344.2040
4047 587.3055 2345.1929
4048 587.3057 2345.1937
4049 587.3067 2345.1977
4050 587.5278 2346.0821
4051 783.3745 2347.1017
4052 784.3563 2350.0471
4053 784.3935 2350.1587
4054 1176.5458 2351.0770
4055 784.7014 2351.0824
4056 785.3738 2353.0996
4057 786.4033 2356.1881
4058 591.2890 2361.1269
4059 591.5244 2362.0685
4060 788.3658 2362.0756
4061 1182.5504 2363.0862
4062 1182.5750 2363.1354
4064 789.3897 2365.1473
4065 789.3931 2365.1575
4066 789.6818 2366.0236
4067 789.6998 2366.0776
4068 789.7034 2366.0884
4069 1184.0537 2366.0928
4070 1184.5470 2367.0794
4071 791.0604 2370.1594
4072 791.3896 2371.1470
4073 791.7352 2372.1838
4074 791.7384 2372.1934
4075 594.0576 2372.2013
4076 594.0577 2372.2017
4078 792.3930 2374.1572
4079 793.3754 2377.1044
4080 793.4024 2377.1854
4081 794.0742 2379.2008
4082 794.3925 2380.1557
4083 1191.1068 2380.1990
4084 794.4073 2380.2001
4085 1191.5542 2381.0938
4086 794.7221 2381.1445
4087 1191.5820 2381.1494
4088 795.0314 2382.0724
4089 1192.0734 2382.1322
4090 795.6995 2384.0767
4091 796.6902 2387.0488
4096 799.7361 2396.1865
4097 800.0301 2397.0685
4099 800.3900 2398.1482
4101 600.5594 2398.2085
4102 800.7265 2399.1577
4104 601.3132 2401.2237
4105 801.4191 2401.2355
4106 601.5613 2402.2161
4108 802.3960 2404.1662
4109 802.4119 2404.2139
4110 804.0429 2409.1069
4111 805.1285 2412.3637
4112 805.3591 2413.0555
4113 604.8086 2415.2053
4114 604.8128 2415.2221
4116 807.0594 2418.1564
4119 809.0394 2424.0964
4121 809.3962 2425.1668
4122 809.4006 2425.1800
4123 809.7774 2426.3104
4124 810.7042 2429.0908
4125 811.3659 2431.0759
4126 608.8111 2431.2153
4127 813.7068 2438.0986
4129 814.0682 2439.1828
4130 814.0865 2439.2377
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