MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3901 738.3375 2211.9907
3902 1107.4937 2212.9728
3903 738.6654 2212.9744
3904 1107.9895 2213.9644
3907 739.6576 2215.9510
3908 1109.5243 2217.0340
3909 740.3569 2218.0489
3910 1110.0332 2218.0518
3911 740.3594 2218.0564
3912 741.3414 2221.0024
3913 1111.5085 2221.0024
3914 1111.5171 2221.0196
3915 741.6863 2222.0371
3916 1112.5256 2223.0366
3917 1114.5282 2227.0418
3918 1114.5334 2227.0522
3919 743.3591 2227.0555
3920 743.6632 2227.9678
3921 1115.9836 2229.9526
3922 744.6582 2230.9528
3923 1117.4828 2232.9510
3924 1117.5256 2233.0366
3925 745.6811 2234.0215
3926 1118.0193 2234.0240
3927 747.3363 2238.9871
3928 1120.5016 2238.9886
3929 1120.5259 2239.0372
3930 747.3961 2239.1665
3931 1120.9895 2239.9644
3932 748.9950 2243.9632
3933 1124.1262 2246.2378
3934 750.0891 2247.2455
3935 1125.0298 2248.0450
3936 750.3763 2248.1071
3937 750.7043 2249.0911
3939 1126.0614 2250.1082
3940 751.0512 2250.1318
3941 1126.5408 2251.0670
3942 751.3774 2251.1104
3943 751.3780 2251.1122
3944 751.3793 2251.1161
3945 1127.0417 2252.0688
3946 751.7771 2252.3095
3947 752.0129 2253.0169
3948 1127.5160 2253.0174
3949 752.3389 2253.9949
3950 752.3733 2254.0981
3951 1130.5670 2259.1194
3952 755.3866 2263.1380
3953 756.4012 2266.1818
3955 1134.5625 2267.1104
3956 1135.5062 2268.9978
3957 757.3426 2269.0060
3958 1135.5132 2269.0118
3959 757.6711 2269.9915
3960 1136.5529 2271.0912
3961 758.3364 2271.9874
3962 1137.0013 2271.9880
3963 758.3519 2272.0339
3964 1137.5344 2273.0542
3965 1138.5421 2275.0696
3966 761.0687 2280.1843
3968 761.4020 2281.1842
3969 1141.6047 2281.1948
3970 1143.5283 2285.0420
3972 1144.5421 2287.0696
3973 1145.5804 2289.1462
3975 764.7162 2291.1268
3976 1146.5721 2291.1296
3977 1146.5811 2291.1476
3978 765.0558 2292.1456
3979 765.0859 2292.2359
3980 765.4164 2293.2274
3981 765.4192 2293.2358
3982 765.4203 2293.2391
3983 765.4205 2293.2397
3984 765.4208 2293.2406
3985 765.4210 2293.2412
3986 765.4216 2293.2430
3987 765.4241 2293.2505
3988 765.7562 2294.2468
3989 1149.0104 2296.0062
3990 1149.5237 2297.0328
3991 767.0192 2298.0358
3993 1151.5706 2301.1266
3996 768.3812 2302.1218
3999 1152.5846 2303.1546
4000 1153.5839 2305.1532
4001 769.3934 2305.1584
4002 1154.0752 2306.1358
4003 769.9925 2306.9557
4004 1154.5551 2307.0956
4005 1154.5702 2307.1258
4006 1154.5731 2307.1316
4007 770.0523 2307.1351
4008 770.0709 2307.1909
4009 1155.0723 2308.1300
4010 770.3845 2308.1317
4011 770.3853 2308.1341
4012 771.7114 2312.1124
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