| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 3 |
| MS data file | : | PRT1245_Other_P1_B11.mgf |
| Database | : | S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues) |
| Timestamp | : | 24 Jun 2025 at 21:21:58 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,722 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 668.8079 | 1335.6012 | 1335.6227 | -16.1 | 0 | 3 | 1 | 1Score > 21 indicates identityScore > 16 indicates homology |
MALGNEINITNK + 3 Deamidated (NQ); Oxidation (M) | |
| 1165.5428 | 1164.5355 | 1164.5332 | 1.97 | 1 | 3 | 1 | 1Score > 21 indicates identityScore > 16 indicates homology |
NMTKIDSPDK + Deamidated (NQ); Oxidation (M) | |
| 880.4233 | 1758.8320 | 1758.8649 | -18.7 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 16 indicates homology |
IQQHNTILNSSSDFR | |
| 980.4718 | 979.4645 | 979.4467 | 18.2 | 0 | 3 | 1 | 1Score > 21 indicates identityScore > 16 indicates homology |
NQMAMEIK + Oxidation (M) | |
| 656.8344 | 1311.6542 | 1311.6744 | -15.4 | 1 | 3 | 1 | 1Score > 22 indicates identityScore > 16 indicates homology |
DVKSQISMIFK + Deamidated (NQ); Oxidation (M) | |
| 453.7202 | 905.4258 | 905.4090 | 18.6 | 1 | 3 | 0.86 | 1Score > 23 indicates identityScore > 15 indicates homology |
QDASDDKK | |
| 685.0165 | 2052.0277 | 2052.0561 | -13.9 | 1 | 3 | 1 | 1Score > 22 indicates identityScore > 16 indicates homology |
VINKCTSDIQLNSGIVYK + Deamidated (NQ) | |
| 762.7337 | 2285.1793 | 2285.1831 | -1.67 | 1 | 3 | 1 | 1Score > 21 indicates identityScore > 16 indicates homology |
EFVKLEQSLVLYFGDQLEK + Deamidated (NQ) | |
| 615.3472 | 614.3399 | 614.3388 | 1.87 | 0 | 3 | 1.7 | 1Score > 18 indicates identity |
GGLSPGK | |
| 853.4541 | 852.4468 | 852.4527 | -6.94 | 1 | 3 | 2.3 | 1Score > 19 indicates identity |
KIASFCK | |
| 801.4077 | 2401.2013 | 2401.2224 | -8.79 | 0 | 3 | 1 | 1Score > 23 indicates identityScore > 16 indicates homology |
DISQLTDLLSINDVIGQATLNR + 3 Deamidated (NQ) | |
| 722.3955 | 721.3882 | 721.3759 | 17.1 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 16 indicates homology |
GTIFER | |
| 984.4807 | 1966.9468 | 1966.9346 | 6.23 | 1 | 3 | 1 | 1Score > 23 indicates identityScore > 16 indicates homology |
ANPNPVKFMNTSEIFQK + 3 Deamidated (NQ) | |
| 997.4601 | 996.4528 | 996.4625 | -9.67 | 0 | 3 | 0.93 | 1Score > 20 indicates identityScore > 15 indicates homology |
DNPPNGEVR | |
| 712.3384 | 2133.9934 | 2133.9749 | 8.66 | 1 | 3 | 0.65 | 1Score > 21 indicates identityScore > 14 indicates homology |
GLEINDYGQNMHRISNVR + 3 Deamidated (NQ); Oxidation (M) | |
| 948.4359 | 947.4286 | 947.4447 | -17.0 | 0 | 3 | 1 | 1Score > 21 indicates identityScore > 16 indicates homology |
SLENDLEK + Deamidated (NQ) | |
| 1049.5132 | 1048.5059 | 1048.4866 | 18.5 | 0 | 3 | 1 | 1Score > 23 indicates identityScore > 16 indicates homology |
GPFNWTVEV + Deamidated (NQ) | |
| 655.3246 | 1308.6346 | 1308.6323 | 1.79 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 16 indicates homology |
ANLHGYGNPNPR | |
| 668.3375 | 1334.6604 | 1334.6387 | 16.3 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 16 indicates homology |
VENGIMQSISEK + Deamidated (NQ) | |
| 1059.5126 | 1058.5053 | 1058.5066 | -1.21 | 0 | 3 | 1 | 1Score > 21 indicates identityScore > 16 indicates homology |
LSNEMHALK + Deamidated (NQ); Oxidation (M) | |
| 992.8338 | 2975.4796 | 2975.4288 | 17.1 | 0 | 3 | 0.88 | 1Score > 21 indicates identityScore > 15 indicates homology |
TIHIIEVDESFDATLSDDVVDQWLSK + Deamidated (NQ) | |
| 564.3004 | 1689.8794 | 1689.8542 | 14.9 | 1 | 3 | 1 | 1Score > 21 indicates identityScore > 16 indicates homology |
KIICPSVESISNGMR | |
| 671.3409 | 2011.0009 | 2011.0149 | -6.99 | 1 | 3 | 0.8 | 1Score > 22 indicates identityScore > 15 indicates homology |
LYQEEDLKNFEIQTLK + Deamidated (NQ) | |
| 768.3791 | 2302.1155 | 2302.1297 | -6.18 | 1 | 3 | 0.57 | 1Score > 22 indicates identityScore > 13 indicates homology |
LIHMERDLSSEVVGQMDAIK + 2 Oxidation (M) | |
| 708.3804 | 707.3731 | 707.3602 | 18.2 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 16 indicates homology |
ATVNFR + Deamidated (NQ) | |
| 752.8972 | 1503.7798 | 1503.7722 | 5.11 | 0 | 3 | 0.98 | 1Score > 22 indicates identityScore > 15 indicates homology |
VSFWEETILQPR | |
| 705.3397 | 2112.9973 | 2113.0224 | -11.9 | 1 | 3 | 0.59 | 1Score > 21 indicates identityScore > 13 indicates homology |
IDMYKEQGFGNLPICIAK + Deamidated (NQ); Oxidation (M) | |
| 965.1835 | 2892.5287 | 2892.5848 | -19.4 | 1 | 3 | 1.9 | 1Score > 18 indicates identity |
LQIGEVVTTKPTIGFNVETLSYKNLK + Deamidated (NQ) | |
| 372.6858 | 743.3570 | 743.3490 | 10.8 | 0 | 3 | 2.2 | 1Score > 19 indicates identity |
EGTYFK | |
| 494.2554 | 986.4962 | 986.5032 | -7.08 | 0 | 3 | 0.55 | 1Score > 22 indicates identityScore > 13 indicates homology |
QLGNNEIAK + Deamidated (NQ) | |
| 657.3581 | 656.3508 | 656.3606 | -14.8 | 0 | 3 | 1.2 | 1Score > 16 indicates identity |
QPSIGR | |
| 687.3553 | 2059.0441 | 2059.0684 | -11.8 | 1 | 3 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
QLLEEVGLSSAGISPKQSSK + 2 Deamidated (NQ) | |
| 999.4766 | 998.4693 | 998.4781 | -8.82 | 0 | 3 | 2.3 | 1Score > 19 indicates identity |
EGVGPNVGDR | |
| 361.7015 | 721.3884 | 721.3911 | -3.74 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
FLTWR | |
| 523.9301 | 1568.7685 | 1568.7617 | 4.33 | 0 | 3 | 0.66 | 1Score > 21 indicates identityScore > 14 indicates homology |
MNNTSITGPQVLHR + 2 Deamidated (NQ) | |
| 892.4647 | 891.4574 | 891.4450 | 13.9 | 0 | 3 | 0.57 | 1Score > 23 indicates identityScore > 13 indicates homology |
LYQESPR | |
| 813.7838 | 2438.3296 | 2438.3395 | -4.08 | 1 | 3 | 0.93 | 1Score > 15 indicates identity |
YKPKVLISQVWNAIIISMYR + Deamidated (NQ); Oxidation (M) | |
| 940.4289 | 1878.8432 | 1878.8451 | -0.99 | 1 | 3 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
VRMQGEEQENIMIER + 2 Deamidated (NQ); Oxidation (M) | |
| 982.8125 | 2945.4157 | 2945.3818 | 11.5 | 1 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
TFRNPVYLTSQTSSSSDYVFDTSLTK + 2 Deamidated (NQ) | |
| 337.1798 | 672.3450 | 672.3555 | -15.5 | 0 | 3 | 2.1 | 1Score > 19 indicates identity |
QAQAAGK | |
| 810.3635 | 809.3562 | 809.3555 | 0.88 | 0 | 3 | 2 | 1Score > 18 indicates identity |
EGANYQK + Deamidated (NQ) | |
| 543.5931 | 1627.7575 | 1627.7399 | 10.8 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
TQGYTVADCTAEAIK + Deamidated (NQ) | |
| 544.2867 | 1086.5588 | 1086.5709 | -11.1 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
EHTIIAEFK | |
| 1068.5300 | 2135.0454 | 2135.0146 | 14.5 | 1 | 3 | 0.75 | 1Score > 23 indicates identityScore > 14 indicates homology |
IQVHYMNYGESIKFFSR + Deamidated (NQ); Oxidation (M) | |
| 695.3431 | 1388.6716 | 1388.6531 | 13.3 | 1 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
NPSRSIADSAQNK + 2 Deamidated (NQ) | |
| 1023.4990 | 1022.4917 | 1022.4815 | 10.0 | 1 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
MSPNSSKTR + Oxidation (M) | |
| 852.4724 | 1702.9302 | 1702.9420 | -6.88 | 0 | 3 | 2.1 | 1Score > 18 indicates identity |
QHIHYLNLLIHFR | |
| 540.2816 | 1078.5486 | 1078.5659 | -16.0 | 0 | 3 | 0.7 | 1Score > 23 indicates identityScore > 14 indicates homology |
STIIENFQK | |
| 1095.5562 | 3283.6468 | 3283.6466 | 0.041 | 1 | 3 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
APVTNTNGVQSQLATNRGVAAILAMGTATPSNR + 3 Deamidated (NQ) | |
| 982.8144 | 2945.4214 | 2945.4667 | -15.4 | 0 | 3 | 0.68 | 1Score > 22 indicates identityScore > 13 indicates homology |
TLPAMKPELFDINNDHNLPMSLYIK + 2 Oxidation (M) | |
| 850.1027 | 2547.2863 | 2547.2964 | -3.98 | 1 | 3 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
WQMELLMLDATGKEVEPTILSK + Oxidation (M) | |
| 634.3571 | 633.3498 | 633.3520 | -3.39 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
VGSLMK | |
| 454.7281 | 907.4416 | 907.4433 | -1.82 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
TSLNIMGR + Deamidated (NQ); Oxidation (M) | |
| 718.3708 | 2152.0906 | 2152.1060 | -7.18 | 0 | 3 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
NIVMAHSLGSYLMALYLQK + Deamidated (NQ) | |
| 992.8273 | 2975.4601 | 2975.5128 | -17.7 | 1 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
TLTHALIDQIVNDPILKTDTDYYDAK | |
| 757.9160 | 1513.8174 | 1513.8075 | 6.58 | 1 | 3 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
KPTMIKLQFHNR + 2 Deamidated (NQ) | |
| 503.7527 | 1005.4908 | 1005.5025 | -11.6 | 1 | 3 | 0.63 | 1Score > 23 indicates identityScore > 13 indicates homology |
REVGASMTR | |
| 896.4211 | 895.4138 | 895.4035 | 11.5 | 0 | 3 | 1 | 1Score > 20 indicates identityScore > 15 indicates homology |
SIDENYR | |
| 991.4755 | 990.4682 | 990.4627 | 5.62 | 0 | 3 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
SPCVMIER | |
| 510.5842 | 1528.7308 | 1528.7481 | -11.3 | 1 | 2 | 0.66 | 1Score > 21 indicates identityScore > 13 indicates homology |
GEQGAIDIKGGNVNR + 2 Deamidated (NQ) | |
| 693.3600 | 2077.0582 | 2077.0613 | -1.50 | 0 | 2 | 0.82 | 1Score > 22 indicates identityScore > 14 indicates homology |
TSIMLGLGETDEQITQTLK | |
| 697.3891 | 696.3818 | 696.3919 | -14.4 | 1 | 2 | 0.97 | 1Score > 15 indicates identity |
GEVHKK | |
| 851.3919 | 850.3846 | 850.4007 | -18.9 | 1 | 2 | 2.6 | 1Score > 19 indicates identity |
YEKMHK + Oxidation (M) | |
| 590.2883 | 1178.5620 | 1178.5390 | 19.6 | 0 | 2 | 1 | 1Score > 23 indicates identityScore > 15 indicates homology |
AAFTPEMQER | |
| 665.3765 | 1328.7384 | 1328.7299 | 6.40 | 1 | 2 | 0.68 | 1Score > 20 indicates identityScore > 13 indicates homology |
GKSAEIDASKPVK | |
| 570.2728 | 1138.5310 | 1138.5506 | -17.2 | 1 | 2 | 0.68 | 1Score > 21 indicates identityScore > 13 indicates homology |
AGDKYSIEQK + Deamidated (NQ) | |
| 1154.5653 | 1153.5580 | 1153.5657 | -6.67 | 1 | 2 | 0.9 | 1Score > 22 indicates identityScore > 14 indicates homology |
MNMKMLLTR + Deamidated (NQ); Oxidation (M) | |
| 527.7445 | 1053.4744 | 1053.4760 | -1.51 | 1 | 2 | 1 | 1Score > 20 indicates identityScore > 15 indicates homology |
MSKDLNSSR + Deamidated (NQ); Oxidation (M) | |
| 584.3410 | 583.3337 | 583.3442 | -17.9 | 0 | 2 | 0.58 | 1Score > 13 indicates identity |
VAAPAR | |
| 431.2200 | 860.4254 | 860.4392 | -16.0 | 0 | 2 | 0.95 | 1Score > 23 indicates identityScore > 15 indicates homology |
VSDLYHK | |
| 517.7678 | 1033.5210 | 1033.5193 | 1.73 | 0 | 2 | 1 | 1Score > 24 indicates identityScore > 15 indicates homology |
QPFTVSNNK | |
| 1170.0535 | 2338.0924 | 2338.0560 | 15.6 | 0 | 2 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
QGDHPNEEENIILSNGIQTAR + 4 Deamidated (NQ) | |
| 1039.4961 | 3115.4665 | 3115.5230 | -18.2 | 1 | 2 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
LENDLNEELVGQLSKTMDNLENLTIPR + 2 Deamidated (NQ); Oxidation (M) | |
| 460.7156 | 919.4166 | 919.4255 | -9.67 | 1 | 2 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
KHMTEMK + Oxidation (M) | |
| 782.3720 | 1562.7294 | 1562.7424 | -8.26 | 1 | 2 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
LEGRGTETEESINK + Deamidated (NQ) | |
| 706.0266 | 2115.0580 | 2115.0556 | 1.13 | 1 | 2 | 0.66 | 1Score > 23 indicates identityScore > 13 indicates homology |
NVSGQDVAAALEANAKSVESR | |
| 898.9739 | 1795.9332 | 1795.9026 | 17.1 | 1 | 2 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
IEQNSIMKNGFGTVLK + 2 Deamidated (NQ); Oxidation (M) | |
| 558.2607 | 1114.5068 | 1114.5155 | -7.81 | 0 | 2 | 2.2 | 1Score > 18 indicates identity |
INQWSSHSR + Deamidated (NQ) | |
| 794.3690 | 793.3617 | 793.3640 | -2.89 | 0 | 2 | 0.97 | 1Score > 21 indicates identityScore > 15 indicates homology |
TTCGDLK | |
| 544.3099 | 543.3026 | 543.3016 | 1.82 | 1 | 2 | 1.8 | 1Score > 17 indicates identity |
KNGPK + Deamidated (NQ) | |
| 1093.5088 | 1092.5015 | 1092.4948 | 6.14 | 0 | 2 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
QPPETNHNR + Deamidated (NQ) | |
| 519.7714 | 1037.5282 | 1037.5366 | -8.07 | 1 | 2 | 0.85 | 1Score > 21 indicates identityScore > 14 indicates homology |
QNKVQHQR + Deamidated (NQ) | |
| 334.1929 | 666.3712 | 666.3813 | -15.1 | 0 | 2 | 1.6 | 1Score > 17 indicates identity |
GLPPQR | |
| 770.3658 | 1538.7170 | 1538.7073 | 6.33 | 0 | 2 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
RPDNQNPEGENLR + Deamidated (NQ) | |
| 802.4180 | 1602.8214 | 1602.8478 | -16.4 | 1 | 2 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
AGPGSQLVIGRYTER | |
| 1072.0841 | 2142.1536 | 2142.1783 | -11.5 | 1 | 2 | 2.2 | 1Score > 18 indicates identity |
SSDITAILPTEQSQKLVLAK + Deamidated (NQ) | |
| 512.2472 | 1022.4798 | 1022.4669 | 12.7 | 0 | 2 | 0.82 | 1Score > 22 indicates identityScore > 14 indicates homology |
LGGENNYEK | |
| 657.3278 | 1968.9616 | 1968.9350 | 13.5 | 0 | 2 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
MSNTEELIQNSIGFLQK + 2 Deamidated (NQ); Oxidation (M) | |
| 448.2365 | 894.4584 | 894.4672 | -9.74 | 0 | 2 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
QPGHLTSR | |
| 795.3864 | 794.3791 | 794.3922 | -16.5 | 0 | 2 | 1 | 1Score > 22 indicates identityScore > 15 indicates homology |
GLNTAYR + Deamidated (NQ) | |
| 968.4681 | 2902.3825 | 2902.3616 | 7.18 | 0 | 2 | 0.85 | 1Score > 21 indicates identityScore > 14 indicates homology |
QVLEYSSPTATTPMTPTFPSTSGINMK + Deamidated (NQ); Oxidation (M) | |
| 778.3749 | 777.3676 | 777.3691 | -1.85 | 1 | 2 | 1 | 1Score > 21 indicates identityScore > 15 indicates homology |
KLDNCK + Deamidated (NQ) | |
| 551.2828 | 1100.5510 | 1100.5713 | -18.4 | 1 | 2 | 0.72 | 1Score > 21 indicates identityScore > 13 indicates homology |
TIEPQEEKK | |
| 898.4499 | 897.4426 | 897.4556 | -14.4 | 0 | 2 | 1 | 1Score > 20 indicates identityScore > 15 indicates homology |
LSNNLHAK + 2 Deamidated (NQ) | |
| 456.2332 | 910.4518 | 910.4508 | 1.11 | 0 | 2 | 3.2 | 1Score > 20 indicates identity |
DLEVNGHK | |
| 790.9107 | 1579.8068 | 1579.8181 | -7.12 | 1 | 2 | 1 | 1Score > 22 indicates identityScore > 14 indicates homology |
KTLLWVNGGGTFCK | |
| 968.4800 | 967.4727 | 967.4545 | 18.8 | 1 | 2 | 1 | 1Score > 21 indicates identityScore > 14 indicates homology |
FMEQKNR + Oxidation (M) | |
| 542.7680 | 1083.5214 | 1083.5019 | 18.1 | 1 | 2 | 1 | 1Score > 21 indicates identityScore > 14 indicates homology |
ENGVCKSYK | |
| 524.7634 | 1047.5122 | 1047.5270 | -14.1 | 0 | 2 | 1 | 1Score > 22 indicates identityScore > 14 indicates homology |
AMSVTPLNAK + Deamidated (NQ); Oxidation (M) | |
| 889.4397 | 1776.8648 | 1776.8936 | -16.2 | 1 | 2 | 1 | 1Score > 22 indicates identityScore > 14 indicates homology |
ILQMGAIMNLGMPSRK + 2 Deamidated (NQ); Oxidation (M) |
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