MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 4645)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2612 668.8079 1335.6012 1335.6227 -16.1 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
MALGNEINITNK + 3 Deamidated (NQ); Oxidation (M)
2367 1165.5428 1164.5355 1164.5332 1.97 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
NMTKIDSPDK + Deamidated (NQ); Oxidation (M)
3189 880.4233 1758.8320 1758.8649 -18.7 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
IQQHNTILNSSSDFR
1661 980.4718 979.4645 979.4467 18.2 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
NQMAMEIK + Oxidation (M)
2583 656.8344 1311.6542 1311.6744 -15.4 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
DVKSQISMIFK + Deamidated (NQ); Oxidation (M)
1448 453.7202 905.4258 905.4090 18.6 1 3 0.86 +1Score > 23 indicates identity
Score > 15 indicates homology
QDASDDKK
3578 685.0165 2052.0277 2052.0561 -13.9 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
VINKCTSDIQLNSGIVYK + Deamidated (NQ)
3971 762.7337 2285.1793 2285.1831 -1.67 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
EFVKLEQSLVLYFGDQLEK + Deamidated (NQ)
1002 615.3472 614.3399 614.3388 1.87 0 3 1.7 +1Score > 18 indicates identity GGLSPGK
1326 853.4541 852.4468 852.4527 -6.94 1 3 2.3 +1Score > 19 indicates identity KIASFCK
4103 801.4077 2401.2013 2401.2224 -8.79 0 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
DISQLTDLLSINDVIGQATLNR + 3 Deamidated (NQ)
1109 722.3955 721.3882 721.3759 17.1 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
GTIFER
3447 984.4807 1966.9468 1966.9346 6.23 1 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
ANPNPVKFMNTSEIFQK + 3 Deamidated (NQ)
1718 997.4601 996.4528 996.4625 -9.67 0 3 0.93 +1Score > 20 indicates identity
Score > 15 indicates homology
DNPPNGEVR
3791 712.3384 2133.9934 2133.9749 8.66 1 3 0.65 +1Score > 21 indicates identity
Score > 14 indicates homology
GLEINDYGQNMHRISNVR + 3 Deamidated (NQ); Oxidation (M)
1573 948.4359 947.4286 947.4447 -17.0 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
SLENDLEK + Deamidated (NQ)
1900 1049.5132 1048.5059 1048.4866 18.5 0 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
GPFNWTVEV + Deamidated (NQ)
2580 655.3246 1308.6346 1308.6323 1.79 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
ANLHGYGNPNPR
2611 668.3375 1334.6604 1334.6387 16.3 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
VENGIMQSISEK + Deamidated (NQ)
1940 1059.5126 1058.5053 1058.5066 -1.21 0 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
LSNEMHALK + Deamidated (NQ); Oxidation (M)
4411 992.8338 2975.4796 2975.4288 17.1 0 3 0.88 +1Score > 21 indicates identity
Score > 15 indicates homology
TIHIIEVDESFDATLSDDVVDQWLSK + Deamidated (NQ)
3093 564.3004 1689.8794 1689.8542 14.9 1 3 1 +1Score > 21 indicates identity
Score > 16 indicates homology
KIICPSVESISNGMR
3517 671.3409 2011.0009 2011.0149 -6.99 1 3 0.8 +1Score > 22 indicates identity
Score > 15 indicates homology
LYQEEDLKNFEIQTLK + Deamidated (NQ)
3995 768.3791 2302.1155 2302.1297 -6.18 1 3 0.57 +1Score > 22 indicates identity
Score > 13 indicates homology
LIHMERDLSSEVVGQMDAIK + 2 Oxidation (M)
1099 708.3804 707.3731 707.3602 18.2 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
ATVNFR + Deamidated (NQ)
2813 752.8972 1503.7798 1503.7722 5.11 0 3 0.98 +1Score > 22 indicates identity
Score > 15 indicates homology
VSFWEETILQPR
3745 705.3397 2112.9973 2113.0224 -11.9 1 3 0.59 +1Score > 21 indicates identity
Score > 13 indicates homology
IDMYKEQGFGNLPICIAK + Deamidated (NQ); Oxidation (M)
4366 965.1835 2892.5287 2892.5848 -19.4 1 3 1.9 +1Score > 18 indicates identity LQIGEVVTTKPTIGFNVETLSYKNLK + Deamidated (NQ)
1146 372.6858 743.3570 743.3490 10.8 0 3 2.2 +1Score > 19 indicates identity EGTYFK
1689 494.2554 986.4962 986.5032 -7.08 0 3 0.55 +1Score > 22 indicates identity
Score > 13 indicates homology
QLGNNEIAK + Deamidated (NQ)
1042 657.3581 656.3508 656.3606 -14.8 0 3 1.2 +1Score > 16 indicates identity QPSIGR
3595 687.3553 2059.0441 2059.0684 -11.8 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
QLLEEVGLSSAGISPKQSSK + 2 Deamidated (NQ)
1727 999.4766 998.4693 998.4781 -8.82 0 3 2.3 +1Score > 19 indicates identity EGVGPNVGDR
1110 361.7015 721.3884 721.3911 -3.74 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
FLTWR
2912 523.9301 1568.7685 1568.7617 4.33 0 3 0.66 +1Score > 21 indicates identity
Score > 14 indicates homology
MNNTSITGPQVLHR + 2 Deamidated (NQ)
1413 892.4647 891.4574 891.4450 13.9 0 3 0.57 +1Score > 23 indicates identity
Score > 13 indicates homology
LYQESPR
4128 813.7838 2438.3296 2438.3395 -4.08 1 3 0.93 +1Score > 15 indicates identity YKPKVLISQVWNAIIISMYR + Deamidated (NQ); Oxidation (M)
3344 940.4289 1878.8432 1878.8451 -0.99 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
VRMQGEEQENIMIER + 2 Deamidated (NQ); Oxidation (M)
4381 982.8125 2945.4157 2945.3818 11.5 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
TFRNPVYLTSQTSSSSDYVFDTSLTK + 2 Deamidated (NQ)
1057 337.1798 672.3450 672.3555 -15.5 0 3 2.1 +1Score > 19 indicates identity QAQAAGK
1242 810.3635 809.3562 809.3555 0.88 0 3 2 +1Score > 18 indicates identity EGANYQK + Deamidated (NQ)
3011 543.5931 1627.7575 1627.7399 10.8 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
TQGYTVADCTAEAIK + Deamidated (NQ)
2044 544.2867 1086.5588 1086.5709 -11.1 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
EHTIIAEFK
3798 1068.5300 2135.0454 2135.0146 14.5 1 3 0.75 +1Score > 23 indicates identity
Score > 14 indicates homology
IQVHYMNYGESIKFFSR + Deamidated (NQ); Oxidation (M)
2659 695.3431 1388.6716 1388.6531 13.3 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
NPSRSIADSAQNK + 2 Deamidated (NQ)
1806 1023.4990 1022.4917 1022.4815 10.0 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
MSPNSSKTR + Oxidation (M)
3107 852.4724 1702.9302 1702.9420 -6.88 0 3 2.1 +1Score > 18 indicates identity QHIHYLNLLIHFR
2014 540.2816 1078.5486 1078.5659 -16.0 0 3 0.7 +1Score > 23 indicates identity
Score > 14 indicates homology
STIIENFQK
4539 1095.5562 3283.6468 3283.6466 0.041 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
APVTNTNGVQSQLATNRGVAAILAMGTATPSNR + 3 Deamidated (NQ)
4382 982.8144 2945.4214 2945.4667 -15.4 0 3 0.68 +1Score > 22 indicates identity
Score > 13 indicates homology
TLPAMKPELFDINNDHNLPMSLYIK + 2 Oxidation (M)
4202 850.1027 2547.2863 2547.2964 -3.98 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
WQMELLMLDATGKEVEPTILSK + Oxidation (M)
1018 634.3571 633.3498 633.3520 -3.39 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
VGSLMK
1452 454.7281 907.4416 907.4433 -1.82 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
TSLNIMGR + Deamidated (NQ); Oxidation (M)
3831 718.3708 2152.0906 2152.1060 -7.18 0 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
NIVMAHSLGSYLMALYLQK + Deamidated (NQ)
4410 992.8273 2975.4601 2975.5128 -17.7 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
TLTHALIDQIVNDPILKTDTDYYDAK
2829 757.9160 1513.8174 1513.8075 6.58 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
KPTMIKLQFHNR + 2 Deamidated (NQ)
1753 503.7527 1005.4908 1005.5025 -11.6 1 3 0.63 +1Score > 23 indicates identity
Score > 13 indicates homology
REVGASMTR
1419 896.4211 895.4138 895.4035 11.5 0 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
SIDENYR
1694 991.4755 990.4682 990.4627 5.62 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
SPCVMIER
2850 510.5842 1528.7308 1528.7481 -11.3 1 2 0.66 +1Score > 21 indicates identity
Score > 13 indicates homology
GEQGAIDIKGGNVNR + 2 Deamidated (NQ)
3656 693.3600 2077.0582 2077.0613 -1.50 0 2 0.82 +1Score > 22 indicates identity
Score > 14 indicates homology
TSIMLGLGETDEQITQTLK
1088 697.3891 696.3818 696.3919 -14.4 1 2 0.97 +1Score > 15 indicates identity GEVHKK
1320 851.3919 850.3846 850.4007 -18.9 1 2 2.6 +1Score > 19 indicates identity YEKMHK + Oxidation (M)
2428 590.2883 1178.5620 1178.5390 19.6 0 2 1 +1Score > 23 indicates identity
Score > 15 indicates homology
AAFTPEMQER
2603 665.3765 1328.7384 1328.7299 6.40 1 2 0.68 +1Score > 20 indicates identity
Score > 13 indicates homology
GKSAEIDASKPVK
2245 570.2728 1138.5310 1138.5506 -17.2 1 2 0.68 +1Score > 21 indicates identity
Score > 13 indicates homology
AGDKYSIEQK + Deamidated (NQ)
2315 1154.5653 1153.5580 1153.5657 -6.67 1 2 0.9 +1Score > 22 indicates identity
Score > 14 indicates homology
MNMKMLLTR + Deamidated (NQ); Oxidation (M)
1919 527.7445 1053.4744 1053.4760 -1.51 1 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
MSKDLNSSR + Deamidated (NQ); Oxidation (M)
980 584.3410 583.3337 583.3442 -17.9 0 2 0.58 +1Score > 13 indicates identity VAAPAR
1346 431.2200 860.4254 860.4392 -16.0 0 2 0.95 +1Score > 23 indicates identity
Score > 15 indicates homology
VSDLYHK
1844 517.7678 1033.5210 1033.5193 1.73 0 2 1 +1Score > 24 indicates identity
Score > 15 indicates homology
QPFTVSNNK
4040 1170.0535 2338.0924 2338.0560 15.6 0 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
QGDHPNEEENIILSNGIQTAR + 4 Deamidated (NQ)
4491 1039.4961 3115.4665 3115.5230 -18.2 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
LENDLNEELVGQLSKTMDNLENLTIPR + 2 Deamidated (NQ); Oxidation (M)
1477 460.7156 919.4166 919.4255 -9.67 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
KHMTEMK + Oxidation (M)
2906 782.3720 1562.7294 1562.7424 -8.26 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
LEGRGTETEESINK + Deamidated (NQ)
3754 706.0266 2115.0580 2115.0556 1.13 1 2 0.66 +1Score > 23 indicates identity
Score > 13 indicates homology
NVSGQDVAAALEANAKSVESR
3226 898.9739 1795.9332 1795.9026 17.1 1 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
IEQNSIMKNGFGTVLK + 2 Deamidated (NQ); Oxidation (M)
2156 558.2607 1114.5068 1114.5155 -7.81 0 2 2.2 +1Score > 18 indicates identity INQWSSHSR + Deamidated (NQ)
1212 794.3690 793.3617 793.3640 -2.89 0 2 0.97 +1Score > 21 indicates identity
Score > 15 indicates homology
TTCGDLK
957 544.3099 543.3026 543.3016 1.82 1 2 1.8 +1Score > 17 indicates identity KNGPK + Deamidated (NQ)
2063 1093.5088 1092.5015 1092.4948 6.14 0 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
QPPETNHNR + Deamidated (NQ)
1859 519.7714 1037.5282 1037.5366 -8.07 1 2 0.85 +1Score > 21 indicates identity
Score > 14 indicates homology
QNKVQHQR + Deamidated (NQ)
1051 334.1929 666.3712 666.3813 -15.1 0 2 1.6 +1Score > 17 indicates identity GLPPQR
2872 770.3658 1538.7170 1538.7073 6.33 0 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
RPDNQNPEGENLR + Deamidated (NQ)
2977 802.4180 1602.8214 1602.8478 -16.4 1 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
AGPGSQLVIGRYTER
3814 1072.0841 2142.1536 2142.1783 -11.5 1 2 2.2 +1Score > 18 indicates identity SSDITAILPTEQSQKLVLAK + Deamidated (NQ)
1804 512.2472 1022.4798 1022.4669 12.7 0 2 0.82 +1Score > 22 indicates identity
Score > 14 indicates homology
LGGENNYEK
3455 657.3278 1968.9616 1968.9350 13.5 0 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
MSNTEELIQNSIGFLQK + 2 Deamidated (NQ); Oxidation (M)
1417 448.2365 894.4584 894.4672 -9.74 0 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
QPGHLTSR
1215 795.3864 794.3791 794.3922 -16.5 0 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
GLNTAYR + Deamidated (NQ)
4368 968.4681 2902.3825 2902.3616 7.18 0 2 0.85 +1Score > 21 indicates identity
Score > 14 indicates homology
QVLEYSSPTATTPMTPTFPSTSGINMK + Deamidated (NQ); Oxidation (M)
1187 778.3749 777.3676 777.3691 -1.85 1 2 1 +1Score > 21 indicates identity
Score > 15 indicates homology
KLDNCK + Deamidated (NQ)
2098 551.2828 1100.5510 1100.5713 -18.4 1 2 0.72 +1Score > 21 indicates identity
Score > 13 indicates homology
TIEPQEEKK
1428 898.4499 897.4426 897.4556 -14.4 0 2 1 +1Score > 20 indicates identity
Score > 15 indicates homology
LSNNLHAK + 2 Deamidated (NQ)
1461 456.2332 910.4518 910.4508 1.11 0 2 3.2 +1Score > 20 indicates identity DLEVNGHK
2937 790.9107 1579.8068 1579.8181 -7.12 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology
KTLLWVNGGGTFCK
1633 968.4800 967.4727 967.4545 18.8 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
FMEQKNR + Oxidation (M)
2031 542.7680 1083.5214 1083.5019 18.1 1 2 1 +1Score > 21 indicates identity
Score > 14 indicates homology
ENGVCKSYK
1891 524.7634 1047.5122 1047.5270 -14.1 0 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology
AMSVTPLNAK + Deamidated (NQ); Oxidation (M)
3208 889.4397 1776.8648 1776.8936 -16.2 1 2 1 +1Score > 22 indicates identity
Score > 14 indicates homology
ILQMGAIMNLGMPSRK + 2 Deamidated (NQ); Oxidation (M)
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