MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3678 1043.4943 2084.9740
3679 696.0065 2084.9977
3680 1043.5101 2085.0056
3681 696.0636 2085.1690
3682 1044.0896 2086.1646
3683 696.9020 2087.6842
3684 1044.9382 2087.8618
3685 697.3286 2088.9640
3686 1045.5000 2088.9854
3687 1045.9911 2089.9676
3688 1045.9915 2089.9684
3689 1046.4956 2090.9766
3690 1046.9868 2091.9590
3691 698.3281 2091.9625
3692 1047.4895 2092.9644
3693 698.9664 2093.8774
3694 524.4941 2093.9473
3695 699.3085 2094.9037
3696 699.3583 2095.0531
3697 1048.9733 2095.9320
3698 525.0043 2095.9881
3699 1049.0378 2096.0610
3700 699.9865 2096.9377
3701 700.0023 2096.9851
3702 1049.5282 2097.0418
3703 1050.0012 2097.9878
3704 1050.5044 2098.9942
3705 1050.5155 2099.0164
3706 1050.5166 2099.0186
3707 701.0140 2100.0202
3708 1051.5050 2100.9954
3711 701.3657 2101.0753
3712 701.3669 2101.0789
3713 701.3676 2101.0810
3714 701.6860 2102.0362
3715 1052.4977 2102.9808
3716 702.3334 2103.9784
3717 1053.4863 2104.9580
3718 702.6647 2104.9723
3719 1053.4949 2104.9752
3720 1053.4949 2104.9752
3721 1053.5095 2105.0044
3722 1053.5203 2105.0260
3723 702.9944 2105.9614
3724 1053.9885 2105.9624
3725 1053.9900 2105.9654
3726 702.9980 2105.9722
3727 703.3323 2106.9751
3728 1054.4989 2106.9832
3729 1055.4818 2108.9490
3730 703.9916 2108.9530
3731 1055.4851 2108.9556
3732 703.9933 2108.9581
3733 1055.5050 2108.9954
3734 1055.5104 2109.0062
3735 1055.5228 2109.0310
3736 1055.5232 2109.0318
3738 1055.5378 2109.0610
3739 1056.5000 2110.9854
3740 704.6907 2111.0503
3741 1056.5359 2111.0572
3742 1056.9692 2111.9238
3743 705.0261 2112.0565
3744 1057.4968 2112.9790
3746 705.3460 2113.0162
3747 1057.5171 2113.0196
3748 705.3495 2113.0267
3749 1057.5234 2113.0322
3750 1058.0048 2113.9950
3751 705.6877 2114.0413
3752 1058.5142 2115.0138
3753 1058.5155 2115.0164
3756 1058.5825 2115.1504
3757 706.0582 2115.1528
3758 706.3416 2116.0030
3759 707.0656 2118.1750
3760 707.3215 2118.9427
3761 707.3320 2118.9742
3762 1060.4949 2118.9752
3763 1060.4952 2118.9758
3764 707.3327 2118.9763
3765 707.6620 2119.9642
3766 707.9952 2120.9638
3767 1061.4896 2120.9646
3768 1061.4919 2120.9692
3769 1061.9845 2121.9544
3770 708.3276 2121.9610
3771 1062.5281 2123.0416
3772 708.6879 2123.0419
3773 1062.5317 2123.0488
3775 1062.5444 2123.0742
3776 708.9979 2123.9719
3777 709.0258 2124.0556
3778 1063.0363 2124.0580
3779 1063.4814 2124.9482
3780 1064.5265 2127.0384
3781 1065.4980 2128.9814
3782 1065.5099 2129.0052
3783 710.9764 2129.9074
3784 1066.0173 2130.0200
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