MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3565 1022.4628 2042.9110
3566 1023.4987 2044.9828
3567 682.6700 2044.9882
3568 1023.9808 2045.9470
3569 1024.4689 2046.9232
3570 683.3156 2046.9250
3571 1024.4797 2046.9448
3573 683.6523 2047.9351
3574 1026.4879 2050.9612
3575 1026.9733 2051.9320
3576 684.9854 2051.9344
3577 1026.9867 2051.9588
3579 1027.0221 2052.0296
3581 686.0069 2054.9989
3582 1028.5093 2055.0040
3583 1028.5094 2055.0042
3584 1028.5326 2055.0506
3585 686.3271 2055.9595
3586 686.3456 2056.0150
3587 1029.4872 2056.9598
3590 686.6786 2057.0140
3591 515.2609 2057.0145
3592 1030.4401 2058.8656
3593 687.2960 2058.8662
3594 1030.5278 2059.0410
3596 687.3555 2059.0447
3597 1030.5343 2059.0540
3598 1030.5367 2059.0588
3599 1030.9312 2059.8478
3600 1031.5011 2060.9876
3601 688.0051 2060.9935
3602 1031.5042 2060.9938
3603 1031.5422 2061.0698
3604 688.3038 2061.8896
3605 1031.9598 2061.9050
3606 688.3107 2061.9103
3607 688.3182 2061.9328
3608 1031.9834 2061.9522
3609 688.6467 2062.9183
3610 1032.4709 2062.9272
3611 1032.4792 2062.9438
3612 1032.4821 2062.9496
3613 1032.5054 2062.9962
3614 688.9825 2063.9257
3615 1033.4733 2064.9320
3617 1033.5060 2064.9974
3619 689.6750 2066.0032
3620 1034.0288 2066.0430
3621 1034.5114 2067.0082
3622 1035.5135 2069.0124
3623 691.3326 2070.9760
3625 1036.9996 2071.9846
3626 1037.4935 2072.9724
3627 1037.5052 2072.9958
3628 1037.5161 2073.0176
3629 1037.5436 2073.0726
3630 1037.5457 2073.0768
3631 1037.5461 2073.0776
3633 692.0354 2073.0844
3634 1037.5502 2073.0858
3635 1037.5569 2073.0992
3636 692.0406 2073.1000
3637 1037.5585 2073.1024
3638 692.3416 2074.0030
3639 1038.0458 2074.0770
3640 692.3666 2074.0780
3641 1038.0464 2074.0782
3643 1038.0494 2074.0842
3644 692.3695 2074.0867
3645 1038.0510 2074.0874
3646 692.3707 2074.0903
3647 1038.0586 2074.1026
3648 1038.0598 2074.1050
3649 692.3872 2074.1398
3650 692.9930 2075.9572
3651 1038.9902 2075.9658
3652 1039.4802 2076.9458
3653 1039.4991 2076.9836
3654 693.3384 2076.9934
3657 1039.9403 2077.8660
3658 693.6326 2077.8760
3659 1039.9558 2077.8970
3660 1039.9696 2077.9246
3661 693.6573 2077.9501
3662 693.9794 2078.9164
3663 1040.4760 2078.9374
3664 693.9866 2078.9380
3665 1040.9746 2079.9346
3666 1041.9988 2081.9830
3667 1042.0020 2081.9894
3668 695.0044 2081.9914
3669 1042.5060 2082.9974
3670 1042.5082 2083.0018
3671 695.3416 2083.0030
3672 1042.5238 2083.0330
3673 695.3523 2083.0351
3674 1043.0219 2084.0292
3675 1043.4836 2084.9526
3676 695.9966 2084.9680
3677 1043.4941 2084.9736
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