MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3450 656.6656 1966.9750
3451 984.5005 1966.9864
3452 984.9843 1967.9540
3454 985.4862 1968.9578
3456 657.3298 1968.9676
3457 985.4932 1968.9718
3458 985.4937 1968.9728
3459 987.4771 1972.9396
3460 987.9858 1973.9570
3461 659.3198 1974.9376
3462 990.4626 1978.9106
3463 990.4808 1978.9470
3464 991.4464 1980.8782
3465 661.3099 1980.9079
3471 662.3220 1983.9442
3472 992.9795 1983.9444
3473 992.9797 1983.9448
3474 993.4840 1984.9534
3476 995.4524 1988.8902
3477 995.4639 1988.9132
3478 498.2389 1988.9265
3479 995.4706 1988.9266
3480 995.4828 1988.9510
3481 995.4839 1988.9532
3482 664.3072 1989.8998
3483 664.3174 1989.9304
3484 996.4551 1990.8956
3485 996.4622 1990.9098
3486 665.3291 1992.9655
3487 997.5043 1992.9940
3488 998.4642 1994.9138
3489 665.9828 1994.9266
3490 998.4744 1994.9342
3492 998.4857 1994.9568
3493 998.5079 1995.0012
3494 999.9656 1997.9166
3495 999.9977 1997.9808
3496 1000.9780 1999.9414
3497 1001.4661 2000.9176
3498 1002.4738 2002.9330
3500 1002.9489 2003.8832
3501 668.9700 2003.8882
3502 1003.0482 2004.0818
3503 669.2965 2004.8677
3504 1003.4550 2004.8954
3505 1003.4642 2004.9138
3506 669.3441 2005.0105
3507 669.6406 2005.9000
3508 669.6681 2005.9825
3509 1004.4721 2006.9296
3510 1004.9608 2007.9070
3511 1004.9698 2007.9250
3512 670.3690 2008.0852
3513 1005.4980 2008.9814
3514 670.6683 2008.9831
3515 1005.4993 2008.9840
3516 1005.5147 2009.0148
3518 1006.9999 2011.9852
3519 1007.0101 2012.0056
3520 1008.4697 2014.9248
3521 1008.9645 2015.9144
3522 1009.4640 2016.9134
3524 674.0284 2019.0634
3525 674.0290 2019.0652
3526 1010.5463 2019.0780
3527 1010.7111 2019.4076
3528 674.6276 2020.8610
3529 1011.4471 2020.8796
3530 674.6343 2020.8811
3532 1011.4713 2020.9280
3533 1011.4715 2020.9284
3535 674.9703 2021.8891
3536 675.6658 2023.9756
3537 1013.4826 2024.9506
3538 1013.4932 2024.9718
3539 1013.4935 2024.9724
3540 675.9991 2024.9755
3541 1013.5078 2025.0010
3542 1013.5081 2025.0016
3543 1014.5034 2026.9922
3544 677.6840 2030.0302
3545 677.6865 2030.0377
3546 678.3192 2031.9358
3547 678.6343 2032.8811
3548 1017.4861 2032.9576
3549 1018.4838 2034.9530
3550 1018.4919 2034.9692
3551 679.6545 2035.9417
3552 1020.9562 2039.8978
3553 680.9954 2039.9644
3554 681.0117 2040.0133
3555 1021.0151 2040.0156
3557 1021.4407 2040.8668
3558 1021.4888 2040.9630
3559 681.3284 2040.9634
3560 1021.4919 2040.9692
3561 1021.4997 2040.9848
3562 681.3560 2041.0462
3563 681.3589 2041.0549
3564 681.6850 2042.0332
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