MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3337 936.4495 1870.8844
3338 936.4757 1870.9368
3339 624.9841 1871.9305
3340 937.4477 1872.8808
3341 625.9705 1874.8897
3343 938.9548 1875.8950
3345 940.4535 1878.8924
3346 627.6385 1879.8937
3347 627.9781 1880.9125
3348 942.4589 1882.9032
3349 943.4713 1884.9280
3350 945.4447 1888.8748
3351 630.6467 1888.9183
3352 945.9370 1889.8594
3353 945.9854 1889.9562
3354 631.6310 1891.8712
3355 947.0013 1891.9880
3356 947.4419 1892.8692
3357 474.2270 1892.8789
3359 947.4940 1892.9734
3360 947.4994 1892.9842
3361 947.4997 1892.9848
3362 632.0032 1892.9878
3363 947.5079 1893.0012
3364 632.9923 1895.9551
3365 948.9918 1895.9690
3366 949.4730 1896.9314
3367 475.9577 1899.8017
3368 634.3104 1899.9094
3369 951.4450 1900.8754
3371 953.4402 1904.8658
3372 954.4385 1906.8624
3373 954.4440 1906.8734
3374 954.9337 1907.8528
3375 956.4499 1910.8852
3376 637.9892 1910.9458
3377 956.4803 1910.9460
3378 956.4834 1910.9522
3379 957.4597 1912.9048
3380 957.4625 1912.9104
3381 958.4492 1914.8838
3382 959.4630 1916.9114
3383 960.4485 1918.8824
3384 960.9534 1919.8922
3385 962.0019 1921.9892
3386 964.4773 1926.9400
3387 964.9452 1927.8758
3388 964.9730 1927.9314
3389 643.9920 1928.9542
3390 966.4469 1930.8792
3391 966.4612 1930.9078
3392 644.6501 1930.9285
3393 483.7400 1930.9309
3394 483.7400 1930.9309
3395 966.4735 1930.9324
3396 967.4646 1932.9146
3397 967.9532 1933.8918
3398 645.9711 1934.8915
3400 646.0052 1934.9938
3401 968.9870 1935.9594
3402 969.0090 1936.0034
3403 646.6576 1936.9510
3404 646.6639 1936.9699
3405 969.4944 1936.9742
3406 969.9943 1937.9740
3407 971.4844 1940.9542
3408 647.9948 1940.9626
3409 971.4888 1940.9630
3412 972.9829 1943.9512
3413 649.3196 1944.9370
3414 488.2293 1948.8881
3415 488.2300 1948.8909
3416 975.4528 1948.8910
3417 975.9482 1949.8818
3418 650.9682 1949.8828
3419 650.9694 1949.8864
3420 650.9724 1949.8954
3421 976.0057 1949.9968
3422 976.0167 1950.0188
3423 976.4515 1950.8884
3424 651.3248 1950.9526
3425 976.4885 1950.9624
3427 651.6681 1951.9825
3428 977.0020 1951.9894
3429 651.6710 1951.9912
3430 977.4866 1952.9586
3431 977.4870 1952.9594
3432 977.4989 1952.9832
3433 977.5033 1952.9920
3434 979.9471 1957.8796
3436 980.4484 1958.8822
3437 980.4499 1958.8852
3438 653.9699 1958.8879
3439 653.9702 1958.8888
3440 980.4603 1958.9060
3441 982.4708 1962.9270
3443 982.9483 1963.8820
3446 983.4488 1964.8830
3448 656.6565 1966.9477
3449 984.4870 1966.9594
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