MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3224 898.4492 1794.8838
3225 898.4640 1794.9134
3227 600.2256 1797.6550
3228 600.3354 1797.9844
3229 600.6047 1798.7923
3230 900.5052 1798.9958
3231 900.8787 1799.7428
3232 901.3660 1800.7174
3233 601.2481 1800.7225
3234 901.6968 1801.3790
3235 901.9313 1801.8480
3236 901.9457 1801.8768
3237 903.3915 1804.7684
3238 602.9324 1805.7754
3239 904.4183 1806.8220
3240 604.9651 1811.8735
3241 604.9686 1811.8840
3242 907.3192 1812.6238
3243 605.2161 1812.6265
3244 605.2969 1812.8689
3245 907.4426 1812.8706
3246 908.8756 1815.7366
3247 908.9526 1815.8906
3248 908.9555 1815.8964
3249 606.5800 1816.7182
3250 606.5858 1816.7356
3251 909.8685 1817.7224
3252 910.4421 1818.8696
3253 911.3896 1820.7646
3254 607.9293 1820.7661
3255 911.4747 1820.9348
3259 911.8898 1821.7650
3260 608.3196 1821.9370
3261 911.9856 1821.9566
3263 912.4450 1822.8754
3264 912.9089 1823.8032
3265 913.4324 1824.8502
3266 913.4762 1824.9378
3267 917.4363 1832.8580
3268 917.4407 1832.8668
3269 917.4521 1832.8896
3271 917.4916 1832.9686
3272 917.9396 1833.8646
3273 459.4828 1833.9021
3274 918.9293 1835.8440
3275 918.9349 1835.8552
3276 612.9601 1835.8585
3277 459.9724 1835.8605
3279 919.8887 1837.7628
3280 920.3957 1838.7768
3281 613.9716 1838.8930
3282 614.3002 1839.8788
3283 920.9515 1839.8884
3285 921.4623 1840.9100
3286 615.2468 1842.7186
3287 922.4349 1842.8552
3288 615.3237 1842.9493
3289 922.8817 1843.7488
3290 923.4446 1844.8746
3291 923.4525 1844.8904
3292 923.4727 1844.9308
3293 924.4417 1846.8688
3294 924.4656 1846.9166
3295 616.6466 1846.9180
3296 924.4714 1846.9282
3297 924.9329 1847.8512
3298 616.9919 1847.9539
3299 924.9858 1847.9570
3300 925.4326 1848.8506
3302 618.2863 1851.8371
3303 618.2919 1851.8539
3305 927.4714 1852.9282
3306 927.8290 1853.6434
3307 618.9543 1853.8411
3308 927.9596 1853.9046
3309 618.9756 1853.9050
3310 619.3466 1855.0180
3311 929.4516 1856.8886
3312 929.9423 1857.8700
3313 620.5788 1858.7146
3314 930.4435 1858.8724
3315 620.9700 1859.8882
3316 930.9570 1859.8994
3317 621.2987 1860.8743
3318 931.4869 1860.9592
3320 931.9406 1861.8666
3321 931.9856 1861.9566
3322 932.4425 1862.8704
3323 932.9341 1863.8536
3324 933.4193 1864.8240
3325 933.4341 1864.8536
3326 933.4468 1864.8790
3327 933.4489 1864.8832
3329 933.9314 1865.8482
3330 934.0502 1866.0858
3331 934.4454 1866.8762
3332 934.4489 1866.8832
3333 623.3721 1867.0945
3334 623.3738 1867.0996
3335 935.9535 1869.8924
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