MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3104 849.9171 1697.8196
3105 567.5806 1699.7200
3108 568.9513 1703.8321
3109 853.4157 1704.8168
3110 853.9173 1705.8200
3111 853.9265 1705.8384
3112 854.8843 1707.7540
3113 854.9269 1707.8392
3114 855.3804 1708.7462
3115 855.4095 1708.8044
3116 855.4183 1708.8220
3118 856.4050 1710.7954
3119 572.2411 1713.7015
3120 858.3922 1714.7698
3121 858.4219 1714.8292
3122 573.9493 1718.8261
3123 861.4476 1720.8806
3124 861.8744 1721.7342
3125 862.3748 1722.7350
3126 575.2548 1722.7426
3127 862.4058 1722.7970
3128 575.5822 1723.7248
3129 863.2010 1724.3874
3130 863.3675 1724.7204
3131 863.4120 1724.8094
3132 575.9439 1724.8099
3133 863.9139 1725.8132
3134 576.2974 1725.8704
3135 864.4291 1726.8436
3136 576.9968 1727.9686
3137 577.5652 1729.6738
3138 865.9691 1729.9236
3139 577.6490 1729.9252
3140 866.9224 1731.8302
3141 867.8753 1733.7360
3142 867.8939 1733.7732
3143 867.8956 1733.7766
3144 868.3985 1734.7824
3145 868.4163 1734.8180
3146 868.4256 1734.8366
3149 579.9344 1736.7814
3150 869.4005 1736.7864
3152 871.4135 1740.8124
3153 871.4341 1740.8536
3154 871.4915 1740.9684
3155 871.4922 1740.9698
3156 871.8680 1741.7214
3157 581.5820 1741.7242
3158 871.9067 1741.7988
3159 581.6453 1741.9141
3160 871.9664 1741.9182
3161 871.9683 1741.9220
3164 872.3659 1742.7172
3165 872.3698 1742.7250
3166 872.4258 1742.8370
3167 582.2409 1743.7009
3168 872.8589 1743.7032
3169 873.4401 1744.8656
3170 874.4280 1746.8414
3171 583.3076 1746.9010
3172 583.6419 1747.9039
3173 875.3926 1748.7706
3174 875.8919 1749.7692
3177 876.3962 1750.7778
3178 876.3998 1750.7850
3181 585.6002 1753.7788
3184 878.9911 1755.9676
3185 879.8812 1757.7478
3186 587.2429 1758.7069
3187 880.3644 1758.7142
3188 880.3662 1758.7178
3190 880.4236 1758.8326
3191 587.5721 1759.6945
3193 587.9585 1760.8537
3194 881.4347 1760.8548
3195 588.5934 1762.7584
3196 883.4202 1764.8258
3197 883.8954 1765.7762
3198 886.4276 1770.8406
3199 886.4868 1770.9590
3200 887.4527 1772.8908
3201 888.3611 1774.7076
3202 888.4203 1774.8260
3204 888.4771 1774.9396
3205 592.9776 1775.9110
3206 889.4094 1776.8042
3209 593.9794 1778.9164
3210 890.9540 1779.8934
3211 445.9873 1779.9201
3212 891.4520 1780.8894
3213 892.4393 1782.8640
3214 892.4393 1782.8640
3215 595.5632 1783.6678
3216 595.6385 1783.8937
3217 894.9824 1787.9502
3218 895.9151 1789.8156
3219 896.4109 1790.8072
3220 897.4040 1792.7934
3221 598.9424 1793.8054
3223 898.4364 1794.8582
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