MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2981 804.8605 1607.7064
2982 806.3687 1610.7228
2983 806.3719 1610.7292
2984 538.6041 1612.7905
2985 807.4070 1612.7994
2986 808.4109 1614.8072
2987 808.4181 1614.8216
2988 404.9311 1615.6953
2989 539.5732 1615.6978
2990 404.9321 1615.6993
2991 539.5740 1615.7002
2992 808.9050 1615.7954
2993 808.9122 1615.8098
2994 810.3939 1618.7732
2996 810.4344 1618.8542
2997 810.9067 1619.7988
2998 540.9410 1619.8012
2999 810.9096 1619.8046
3000 811.4122 1620.8098
3001 541.9142 1622.7208
3002 812.4100 1622.8054
3003 812.4125 1622.8104
3004 812.8679 1623.7212
3005 812.9105 1623.8064
3006 812.9497 1623.8848
3007 813.4189 1624.8232
3008 542.9229 1625.7469
3009 814.3936 1626.7726
3010 814.8824 1627.7502
3013 544.2256 1629.6550
3015 816.4158 1630.8170
3016 816.4304 1630.8462
3017 544.9230 1631.7472
3019 816.9055 1631.7964
3020 816.9197 1631.8248
3021 817.4286 1632.8426
3022 817.4362 1632.8578
3023 817.9097 1633.8048
3024 818.3864 1634.7582
3025 818.4131 1634.8116
3026 818.4319 1634.8492
3027 819.3593 1636.7040
3028 547.5959 1639.7659
3029 547.5985 1639.7737
3030 821.4021 1640.7896
3031 821.9038 1641.7930
3032 411.9392 1643.7277
3033 548.9166 1643.7280
3034 823.3601 1644.7056
3035 549.2465 1644.7177
3036 823.4300 1644.8454
3037 823.4321 1644.8496
3038 824.4207 1646.8268
3039 824.4278 1646.8410
3040 824.4291 1646.8436
3042 825.3748 1648.7350
3044 550.9202 1649.7388
3045 825.8845 1649.7544
3046 826.3909 1650.7672
3047 826.4379 1650.8612
3048 826.4387 1650.8628
3049 827.3998 1652.7850
3050 827.8918 1653.7690
3054 829.3605 1656.7064
3055 829.9034 1657.7922
3056 831.3885 1660.7624
3057 555.2573 1662.7501
3058 832.8837 1663.7528
3059 832.9044 1663.7942
3060 833.3721 1664.7296
3063 834.3992 1666.7838
3067 557.9431 1670.8075
3068 836.9369 1671.8592
3069 558.2964 1671.8674
3071 837.8991 1673.7836
3073 838.4221 1674.8296
3075 839.3879 1676.7612
3076 839.3912 1676.7678
3077 840.8871 1679.7596
3079 560.9277 1679.7613
3080 841.9615 1681.9084
3081 842.4279 1682.8412
3082 842.9081 1683.8016
3083 843.3942 1684.7738
3084 843.3950 1684.7754
3085 843.4023 1684.7900
3086 843.4049 1684.7952
3087 844.9174 1687.8202
3088 563.6142 1687.8208
3089 844.9335 1687.8524
3090 845.4104 1688.8062
3091 845.4261 1688.8376
3092 845.4352 1688.8558
3094 846.4284 1690.8422
3095 846.4335 1690.8524
3096 846.4338 1690.8530
3098 846.4498 1690.8850
3099 846.8824 1691.7502
3101 566.2590 1695.7552
3103 849.8747 1697.7348
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