MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2830 758.8696 1515.7246
2831 759.3741 1516.7336
2832 759.3886 1516.7626
2833 506.9091 1517.7055
2834 759.8615 1517.7084
2835 759.8721 1517.7296
2836 759.8827 1517.7508
2837 760.3810 1518.7474
2838 760.8618 1519.7090
2839 761.3369 1520.6592
2840 761.3826 1520.7506
2841 762.3500 1522.6854
2842 762.3882 1522.7618
2843 508.9249 1523.7529
2844 763.3565 1524.6984
2845 509.5846 1525.7320
2847 764.4448 1526.8750
2849 382.9209 1527.6545
2851 765.3730 1528.7314
2852 510.5855 1528.7347
2856 765.9116 1529.8086
2857 767.3588 1532.7030
2858 767.3702 1532.7258
2860 767.4042 1532.7938
2861 767.8558 1533.6970
2862 512.2418 1533.7036
2863 512.5663 1534.6771
2864 768.3616 1534.7086
2865 768.4030 1534.7914
2866 768.8519 1535.6892
2867 769.3370 1536.6594
2871 769.8868 1537.7590
2873 514.5891 1540.7455
2874 515.5482 1543.6228
2876 773.3958 1544.7770
2877 773.8118 1545.6090
2878 773.8188 1545.6230
2879 773.9013 1545.7880
2881 516.2722 1545.7948
2883 773.9095 1545.8044
2885 774.3713 1546.7280
2886 775.3663 1548.7180
2888 776.8959 1551.7772
2889 777.8843 1553.7540
2892 778.8360 1555.6574
2894 519.9189 1556.7349
2895 780.3826 1558.7506
2896 781.3732 1560.7318
2903 781.9071 1561.7996
2908 782.8627 1563.7108
2909 782.8882 1563.7618
2913 785.8832 1569.7518
2915 785.9298 1569.8450
2916 786.9160 1571.8174
2917 786.9211 1571.8276
2918 787.8964 1573.7782
2919 787.9164 1573.8182
2920 787.9189 1573.8232
2921 788.3684 1574.7222
2922 525.9166 1574.7280
2924 788.3812 1574.7478
2925 788.3959 1574.7772
2926 526.2275 1575.6607
2927 789.3953 1576.7760
2928 789.3991 1576.7836
2929 789.8977 1577.7808
2936 790.3881 1578.7616
2938 791.3906 1580.7666
2939 528.2227 1581.6463
2940 791.8869 1581.7592
2941 792.8658 1583.7170
2942 528.9211 1583.7415
2943 528.9267 1583.7583
2944 792.8871 1583.7596
2945 792.8899 1583.7652
2946 397.4422 1585.7397
2947 529.5878 1585.7416
2948 529.5881 1585.7425
2950 794.3867 1586.7588
2953 794.9161 1587.8176
2955 530.8832 1589.6278
2956 795.9122 1589.8098
2957 795.9125 1589.8104
2958 795.9166 1589.8186
2959 796.3692 1590.7238
2962 798.3734 1594.7322
2963 798.3761 1594.7376
2964 798.8857 1595.7568
2965 800.8651 1599.7156
2966 800.8687 1599.7228
2967 534.2488 1599.7246
2968 400.9395 1599.7289
2969 400.9397 1599.7297
2970 534.2510 1599.7312
2971 801.3716 1600.7286
2972 801.4290 1600.8434
2976 535.2798 1602.8176
2978 802.8431 1603.6716
2979 804.4062 1606.7978
2980 804.4094 1606.8042
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