MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2695 717.8888 1433.7630
2696 717.8929 1433.7712
2697 717.8935 1433.7724
2698 718.3460 1434.6774
2700 718.3472 1434.6798
2701 479.2659 1434.7759
2702 719.3352 1436.6558
2704 720.8362 1439.6578
2705 720.8660 1439.7174
2706 721.3211 1440.6276
2707 721.3630 1440.7114
2708 721.8533 1441.6920
2709 482.2282 1443.6628
2710 722.8806 1443.7466
2712 723.8549 1445.6952
2714 482.9134 1445.7184
2716 723.8717 1445.7288
2718 723.8722 1445.7298
2719 723.8723 1445.7300
2722 723.8734 1445.7322
2724 723.8745 1445.7344
2725 723.8754 1445.7362
2726 723.8769 1445.7392
2727 723.8774 1445.7402
2728 723.8790 1445.7434
2729 723.8802 1445.7458
2730 724.3778 1446.7410
2734 725.8792 1449.7438
2735 726.3455 1450.6764
2736 726.3734 1450.7322
2737 726.8251 1451.6356
2738 728.3550 1454.6954
2740 729.3627 1456.7108
2741 486.8903 1457.6491
2742 730.3536 1458.6926
2743 487.2393 1458.6961
2744 730.3887 1458.7628
2747 730.8758 1459.7370
2748 730.9002 1459.7858
2749 487.6032 1459.7878
2750 730.9012 1459.7878
2751 731.8290 1461.6434
2752 731.8544 1461.6942
2754 732.3702 1462.7258
2755 733.3723 1464.7300
2756 490.2083 1467.6031
2762 736.3514 1470.6882
2764 736.3721 1470.7296
2766 737.9068 1473.7990
2767 492.2737 1473.7993
2768 738.4067 1474.7988
2769 739.3696 1476.7246
2770 739.8618 1477.7090
2771 739.8627 1477.7108
2772 741.3799 1480.7452
2773 741.8376 1481.6606
2774 741.8726 1481.7306
2775 742.3752 1482.7358
2776 495.5621 1483.6645
2777 495.5806 1483.7200
2778 495.5811 1483.7215
2779 742.8785 1483.7424
2780 742.9338 1483.8530
2781 743.3651 1484.7156
2782 743.3747 1484.7348
2783 743.8773 1485.7400
2784 744.3435 1486.6724
2786 745.3648 1488.7150
2787 745.8533 1489.6920
2788 745.8603 1489.7060
2789 497.5762 1489.7068
2792 745.8640 1489.7134
2793 746.8574 1491.7002
2794 746.8650 1491.7154
2796 747.8449 1493.6752
2797 747.8765 1493.7384
2798 748.3571 1494.6996
2799 499.6006 1495.7800
2802 500.9117 1499.7133
2803 500.9126 1499.7160
2805 500.9493 1499.8261
2807 751.8711 1501.7276
2808 752.3568 1502.6990
2809 752.3724 1502.7302
2810 752.3795 1502.7444
2811 752.3805 1502.7464
2814 753.3889 1504.7632
2815 753.4042 1504.7938
2816 753.8583 1505.7020
2817 753.8585 1505.7024
2818 754.3168 1506.6190
2819 755.3266 1508.6386
2820 504.2257 1509.6553
2821 504.2528 1509.7366
2822 756.8796 1511.7446
2823 756.8810 1511.7474
2824 756.8825 1511.7504
2825 757.3755 1512.7364
2826 505.5621 1513.6645
2827 379.4240 1513.6669
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