MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 201–300 (out of 4645)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1286 418.2380 834.4614 834.4599 1.82 1 5 0.81 +1Score > 21 indicates identity
Score > 17 indicates homology
KPDGKYK
1196 781.4257 780.4184 780.4170 1.80 0 5 0.48 +1Score > 21 indicates identity
Score > 15 indicates homology
FVYPQK
2379 584.3120 1166.6094 1166.6295 -17.2 0 5 1 +1Score > 22 indicates identity
Score > 18 indicates homology
NTSPDPALKPK
983 586.3210 585.3137 585.3235 -16.6 0 5 1.2 +1Score > 19 indicates identity AVSPGR
2765 737.8652 1473.7158 1473.7385 -15.3 1 5 0.94 +1Score > 22 indicates identity
Score > 17 indicates homology
IGIPQEMIDDAKK + Deamidated (NQ); Oxidation (M)
3974 764.0583 2289.1531 2289.1207 14.1 1 5 1 +1Score > 22 indicates identity
Score > 18 indicates homology
MMNELLQKLPTCYYQTLK + Oxidation (M)
3997 1152.5724 2303.1302 2303.1467 -7.16 1 5 0.88 +1Score > 22 indicates identity
Score > 17 indicates homology
DVGEEKQTLHQIFADSMVIK + Oxidation (M)
4120 809.3668 2425.0786 2425.1049 -10.9 1 5 1.4 +1Score > 19 indicates identity LMENHACNGLTSCKEQLHQR
1715 997.4593 996.4520 996.4625 -10.5 0 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
DNPPNGEVR
1134 370.7014 739.3882 739.3752 17.7 0 5 1.2 +1Score > 18 indicates identity QYSTLK + Deamidated (NQ)
2721 723.8730 1445.7314 1445.7085 15.9 0 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
SHGAVLPSYCSLR
2501 610.8281 1219.6416 1219.6448 -2.60 0 5 0.35 +1Score > 23 indicates identity
Score > 13 indicates homology
AVGNIVNEIYK + Deamidated (NQ)
2643 685.3209 1368.6272 1368.6265 0.56 1 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
SMVKVMLNDNGK + 2 Deamidated (NQ); 2 Oxidation (M)
1264 411.7279 821.4412 821.4317 11.7 0 5 0.41 +1Score > 21 indicates identity
Score > 13 indicates homology
MSSAIVAK + Oxidation (M)
1504 928.4119 927.4046 927.4158 -12.1 1 5 0.94 +1Score > 17 indicates identity RTQNDHR + 2 Deamidated (NQ)
2914 785.8914 1569.7682 1569.7569 7.21 1 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
DIVASFMSRAGSASR + Oxidation (M)
1578 475.2596 948.5046 948.5029 1.88 1 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
GPRTLYDK
2194 562.8291 1123.6436 1123.6601 -14.7 0 5 0.81 +1Score > 16 indicates identity VVVATSLNPPK
3469 991.5035 1980.9924 1980.9792 6.67 1 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
NLDTFLNQLRDFLNEK + 2 Deamidated (NQ)
1064 678.3222 677.3149 677.3245 -14.2 0 5 0.54 +1Score > 22 indicates identity
Score > 14 indicates homology
NANFGR
1381 438.7389 875.4632 875.4726 -10.6 0 5 1 +1Score > 23 indicates identity
Score > 17 indicates homology
HNQKPPR
3442 655.6337 1963.8793 1963.9044 -12.8 0 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology
AANLGGVAVSGLEMAQNSQK + 4 Deamidated (NQ); Oxidation (M)
1506 928.4744 927.4671 927.4774 -11.1 0 5 0.45 +1Score > 20 indicates identity
Score > 14 indicates homology
QSHAVATSK
2891 519.2569 1554.7489 1554.7679 -12.2 0 5 0.57 +1Score > 22 indicates identity
Score > 15 indicates homology
WTVTGTFLTTGDTR
1190 779.4166 778.4093 778.4086 0.97 1 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
RNSFVR + Deamidated (NQ)
3737 704.0237 2109.0493 2109.0187 14.5 1 5 1 +1Score > 23 indicates identity
Score > 17 indicates homology
MKDYSNIVEPTLEDPTLK + Deamidated (NQ); Oxidation (M)
1295 420.7332 839.4518 839.4501 2.08 0 5 1.3 +1Score > 18 indicates identity NATPVNPK
3710 701.3641 2101.0705 2101.0731 -1.27 0 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
WLLELAVETFEDANINPK
3855 1085.5365 2169.0584 2169.0372 9.82 1 5 1 +1Score > 23 indicates identity
Score > 17 indicates homology
LTYDMLSQDKIINNTNNR + Deamidated (NQ); Oxidation (M)
1120 729.3410 728.3337 728.3381 -6.04 0 5 0.37 +1Score > 13 indicates identity TSLDFF
2887 776.8482 1551.6818 1551.6657 10.4 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
NTNIGGMIAECAER + Deamidated (NQ); Oxidation (M)
2960 531.2486 1590.7240 1590.7460 -13.9 0 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
VATMQVWDTAGQER
3467 991.4904 1980.9662 1980.9792 -6.55 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
NLDTFLNQLRDFLNEK + 2 Deamidated (NQ)
1575 948.4569 947.4496 947.4308 19.9 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
NNESNNKK + Deamidated (NQ)
2673 703.3413 1404.6680 1404.6885 -14.5 0 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
NPGSINIYEIER + Deamidated (NQ)
2551 639.3306 1276.6466 1276.6636 -13.3 1 4 1 +1Score > 23 indicates identity
Score > 17 indicates homology
SPTNNNPRIHK
1183 772.4235 771.4162 771.4239 -9.94 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
LGGQEIR
4264 904.0894 2709.2464 2709.1945 19.2 1 4 0.78 +1Score > 21 indicates identity
Score > 16 indicates homology
SGFHNVGNINMMAQQQMQQNRIK + 4 Deamidated (NQ); 2 Oxidation (M)
4117 807.7191 2420.1355 2420.1536 -7.49 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
FSYSPPKEGNYEIVFDTVSNK
1246 406.2206 810.4266 810.4348 -10.0 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
VSPNAAPR
1336 858.4187 857.4114 857.4171 -6.58 0 4 1.4 +1Score > 18 indicates identity YLSNIFT + Deamidated (NQ)
3207 889.4391 1776.8636 1776.8564 4.10 1 4 0.75 +1Score > 22 indicates identity
Score > 16 indicates homology
EQVQQVVMEGKTQEK + Deamidated (NQ); Oxidation (M)
2893 778.9205 1555.8264 1555.8205 3.80 1 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
QKNILPSAVQSNEK + Deamidated (NQ)
2974 801.9199 1601.8252 1601.8382 -8.06 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
STVPIIIRCCIDR
2146 557.2819 1112.5492 1112.5713 -19.9 0 4 0.4 +1Score > 20 indicates identity
Score > 13 indicates homology
DLEEGPTKPK
1751 1006.4833 1005.4760 1005.4740 2.01 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
FANRNNNR + Deamidated (NQ)
2930 789.8994 1577.7842 1577.7533 19.6 1 4 1 +1Score > 23 indicates identity
Score > 17 indicates homology
ERDGSTEETLNSLK
3967 1141.5909 2281.1672 2281.1273 17.5 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
NLAYNIGIHVEGACPQIQQR + Deamidated (NQ)
2353 581.7938 1161.5730 1161.5513 18.7 0 4 1 +1Score > 23 indicates identity
Score > 17 indicates homology
GLLGDSENETK
2739 728.8867 1455.7588 1455.7681 -6.38 1 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
KTTTSPINIPGGNR + Deamidated (NQ)
1962 533.2750 1064.5354 1064.5502 -13.8 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
KNEIIEYR + Deamidated (NQ)
2483 604.3085 1206.6024 1206.6026 -0.15 1 4 1 +1Score > 23 indicates identity
Score > 17 indicates homology
ILSMRNSENK + Oxidation (M)
4442 753.9074 3011.6005 3011.6233 -7.56 1 4 1.2 +1Score > 17 indicates identity IHALHTVISHHYSQKDLIVIFPSDLK + Deamidated (NQ)
3052 828.4097 1654.8048 1654.7910 8.34 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
KSQQHQTQGNQVLR + 4 Deamidated (NQ)
1170 762.3463 761.3390 761.3378 1.65 0 4 1.7 +1Score > 19 indicates identity ENSGPMK
2610 667.8417 1333.6688 1333.6547 10.6 0 4 0.5 +1Score > 22 indicates identity
Score > 14 indicates homology
MALGNEINITNK + Deamidated (NQ); Oxidation (M)
4414 993.1015 2976.2827 2976.2978 -5.08 1 4 1.3 +1Score > 18 indicates identity GSMSVFQSCHTGLAFNQIQGSSSSQRR + 4 Deamidated (NQ); Oxidation (M)
1639 485.7473 969.4800 969.4767 3.42 1 4 1.9 +1Score > 19 indicates identity KDTTGSSFK
4600 888.4207 3549.6537 3549.7144 -17.1 1 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
GVQSPPKPPPSNEISGTIENDVESIKQAADNMAK + 2 Deamidated (NQ)
1545 470.7406 939.4666 939.4709 -4.48 0 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
VERPMHR + Oxidation (M)
1716 499.2333 996.4520 996.4400 12.1 0 4 0.5 +1Score > 20 indicates identity
Score > 13 indicates homology
ADSFINNSK + 2 Deamidated (NQ)
2711 723.3762 1444.7378 1444.7596 -15.0 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
SIVEPSGALSVAGMK
2362 582.2960 1162.5774 1162.5982 -17.9 0 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
LQTNQIFNGK + Deamidated (NQ)
1294 840.4577 839.4504 839.4501 0.38 0 4 1.5 +1Score > 18 indicates identity NATPVNPK
3319 621.3299 1860.9679 1860.9615 3.43 1 4 0.81 +1Score > 21 indicates identity
Score > 15 indicates homology
IDSNIISSPMVSKVEAR + Oxidation (M)
3262 912.4165 1822.8184 1822.8155 1.60 1 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
NNFKIIDSHSMNNSGK + 2 Deamidated (NQ); Oxidation (M)
4625 927.1975 3704.7609 3704.7662 -1.42 1 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
MAHNQPAVTIDLGMVQSIGYVAETDSAVAERLQR + 3 Deamidated (NQ); 2 Oxidation (M)
2015 540.7523 1079.4900 1079.4917 -1.54 1 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
KNDDGSMVAK + Oxidation (M)
1431 899.4955 898.4882 898.4760 13.6 0 4 1.5 +1Score > 18 indicates identity KPVTPNDK + Deamidated (NQ)
2418 589.3042 1176.5938 1176.5887 4.35 1 4 0.69 +1Score > 24 indicates identity
Score > 15 indicates homology
QAKQLGFDDR
2973 801.9177 1601.8208 1601.8382 -10.8 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
STVPIIIRCCIDR
2846 764.3556 1526.6966 1526.7221 -16.7 1 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
GNSMLFTMNRDIK + Deamidated (NQ)
4093 797.4386 2389.2940 2389.3104 -6.87 0 4 1.3 +1Score > 18 indicates identity LQVHLLENNNDLDLTIGLLLK + 2 Deamidated (NQ)
1181 386.2256 770.4366 770.4399 -4.20 0 4 1.5 +1Score > 18 indicates identity GAGGIGALR
3358 631.9680 1892.8822 1892.8682 7.39 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
AMKEMQPQPGLVDIMR + 2 Deamidated (NQ); 3 Oxidation (M)
4668 1138.1057 4548.3937 4548.3726 4.63 1 4 0.61 +1Score > 14 indicates identity GNVCLNILREDWSPALDLQSIITGLLFLFLEPNPNDPLNK
3954 756.7098 2267.1076 2267.0740 14.8 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
VAVQQTQMQAGVDKLDSFER + 2 Deamidated (NQ); Oxidation (M)
3468 991.4933 1980.9720 1980.9792 -3.63 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
NLDTFLNQLRDFLNEK + 2 Deamidated (NQ)
2344 580.7849 1159.5552 1159.5770 -18.7 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
MVPTMFPPPK + Oxidation (M)
2668 700.3984 1398.7822 1398.7653 12.1 1 4 1.9 +1Score > 19 indicates identity QPNLKCIVLTGR + Deamidated (NQ)
1535 469.7158 937.4170 937.4327 -16.7 1 4 2.2 +1Score > 20 indicates identity GEMKSGWK + Oxidation (M)
1762 1008.4815 1007.4742 1007.4672 6.99 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
AVAEAYNNR + Deamidated (NQ)
1271 414.2175 826.4204 826.4185 2.39 0 4 2.1 +1Score > 19 indicates identity DPPSNGLK
3018 816.9001 1631.7856 1631.7712 8.86 1 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
LGQQNNLPKLDMNK + 4 Deamidated (NQ); Oxidation (M)
1440 451.7343 901.4540 901.4393 16.4 0 4 0.81 +1Score > 22 indicates identity
Score > 15 indicates homology
SNVEVQPK + 2 Deamidated (NQ)
4616 916.4536 3661.7853 3661.8363 -13.9 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
SISVVSLFDDSVNLSFPLGSILSSQTQSLSYNTR + Deamidated (NQ)
1213 794.3693 793.3620 793.3640 -2.49 0 4 0.62 +1Score > 21 indicates identity
Score > 14 indicates homology
QLGDSMK + Oxidation (M)
952 529.2993 528.2920 528.3020 -18.9 0 4 1.7 +1Score > 18 indicates identity AVPSR
3588 686.6617 2056.9633 2056.9371 12.7 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
SMTPFSAGNTSSQNKLENK + Deamidated (NQ); Oxidation (M)
1399 442.7286 883.4426 883.4399 3.08 1 4 2.2 +1Score > 19 indicates identity KDIDEHK
2079 1096.5520 1095.5447 1095.5271 16.1 0 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
ISDFVCLDK
1324 427.2303 852.4460 852.4527 -7.85 1 4 2.1 +1Score > 19 indicates identity KIASFCK
2910 783.3691 1564.7236 1564.7524 -18.4 1 4 0.78 +1Score > 22 indicates identity
Score > 15 indicates homology
MQNVCGDMTRILK
3624 1036.5026 2070.9906 2070.9965 -2.84 1 4 0.57 +1Score > 22 indicates identity
Score > 14 indicates homology
KNEVALMNVIETIMYDR + Deamidated (NQ); 2 Oxidation (M)
3041 824.9069 1647.7992 1647.8146 -9.33 0 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
ILQMGAIMNLGMPSR + Deamidated (NQ); Oxidation (M)
1116 725.3946 724.3873 724.3980 -14.7 1 4 1.9 +1Score > 19 indicates identity GNKHLR + Deamidated (NQ)
3278 919.4523 1836.8900 1836.9073 -9.41 1 4 0.5 +1Score > 22 indicates identity
Score > 13 indicates homology
ISDSTLNKILDMSMNR
2073 1095.5247 1094.5174 1094.5131 3.92 0 3 0.73 +1Score > 22 indicates identity
Score > 15 indicates homology
LDSFEENIK + Deamidated (NQ)
2954 794.9249 1587.8352 1587.8290 3.92 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
LGAVTGLDLAQNCKK + Deamidated (NQ)
2242 569.7789 1137.5432 1137.5302 11.5 0 3 0.81 +1Score > 22 indicates identity
Score > 15 indicates homology
SYSVNSEQPK
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