MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2550 639.3174 1276.6202
2552 639.3315 1276.6484
2553 639.3356 1276.6566
2554 640.2953 1278.5760
2556 641.8314 1281.6482
2557 643.3362 1284.6578
2559 644.2963 1286.5780
2560 644.3013 1286.5880
2562 645.2980 1288.5814
2564 430.8782 1289.6128
2565 647.3260 1292.6374
2567 649.3126 1296.6106
2568 649.8231 1297.6316
2569 649.8239 1297.6332
2570 649.8267 1297.6388
2571 434.2115 1299.6127
2572 651.3296 1300.6446
2573 651.3322 1300.6498
2574 651.8068 1301.5990
2575 434.9161 1301.7265
2579 652.3421 1302.6696
2581 655.8901 1309.7656
2582 656.8336 1311.6526
2584 438.8745 1313.6017
2585 658.3085 1314.6024
2586 658.3109 1314.6072
2590 659.3813 1316.7480
2592 660.3760 1318.7374
2593 660.8286 1319.6426
2594 662.8407 1323.6668
2595 662.8415 1323.6684
2596 663.3036 1324.5926
2597 663.3092 1324.6038
2598 664.8119 1327.6092
2600 664.8320 1327.6494
2601 664.8786 1327.7426
2602 664.8786 1327.7426
2605 666.8323 1331.6500
2606 666.8332 1331.6518
2607 667.3435 1332.6724
2608 667.3456 1332.6766
2609 667.3505 1332.6864
2613 669.3281 1336.6416
2616 669.8605 1337.7064
2617 335.9252 1339.6717
2618 447.5650 1339.6732
2620 672.8297 1343.6448
2621 673.2964 1344.5782
2623 674.3300 1346.6454
2624 674.3328 1346.6510
2625 674.8070 1347.5994
2626 450.2075 1347.6007
2627 674.8307 1347.6468
2628 675.3120 1348.6094
2634 454.2304 1359.6694
2635 682.3334 1362.6522
2636 682.3741 1362.7336
2637 682.3746 1362.7346
2638 455.5396 1363.5970
2639 682.8088 1363.6030
2640 683.2997 1364.5848
2641 683.8347 1365.6548
2642 456.5826 1366.7260
2644 685.3489 1368.6832
2645 457.2372 1368.6898
2646 687.3380 1372.6614
2648 688.8454 1375.6762
2649 689.3372 1376.6598
2650 690.3271 1378.6396
2651 690.3538 1378.6930
2652 691.3239 1380.6332
2653 691.8135 1381.6124
2654 692.8563 1383.6980
2655 692.8586 1383.7026
2657 462.5697 1384.6873
2661 695.8369 1389.6592
2663 464.5466 1390.6180
2664 697.8586 1393.7026
2665 699.8752 1397.7358
2666 700.3724 1398.7302
2667 700.3748 1398.7350
2669 701.3383 1400.6620
2670 701.7870 1401.5594
2671 702.8616 1403.7086
2672 469.2144 1404.6214
2674 469.8704 1406.5894
2675 704.8414 1407.6682
2677 707.3477 1412.6808
2678 708.3892 1414.7638
2681 710.8588 1419.7030
2682 710.8590 1419.7034
2683 711.8787 1421.7428
2684 711.8790 1421.7434
2685 712.8439 1423.6732
2686 713.8594 1425.7042
2687 714.3248 1426.6350
2688 715.8352 1429.6558
2689 715.8360 1429.6574
2690 715.8696 1429.7246
2692 716.3881 1430.7616
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