MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2413 588.8062 1175.5978
2414 588.8241 1175.6336
2415 1177.5527 1176.5454
2416 1177.5630 1176.5557
2417 589.2878 1176.5610
2419 1178.5592 1177.5519
2420 1178.5640 1177.5567
2421 1178.5719 1177.5646
2422 589.7944 1177.5742
2424 589.7961 1177.5776
2426 590.2875 1178.5604
2430 1180.5614 1179.5541
2431 1180.5696 1179.5623
2432 591.2818 1180.5490
2433 1181.5568 1180.5495
2434 1182.5492 1181.5419
2435 591.8121 1181.6096
2436 591.8134 1181.6122
2437 591.8135 1181.6124
2438 592.2724 1182.5302
2439 1183.5431 1182.5358
2440 1183.5461 1182.5388
2441 1183.5618 1182.5545
2443 1184.5437 1183.5364
2444 1184.5629 1183.5556
2445 592.7877 1183.5608
2446 1184.5687 1183.5614
2447 592.7908 1183.5670
2448 1184.5747 1183.5674
2449 593.7814 1185.5482
2450 1186.5596 1185.5523
2451 1188.5453 1187.5380
2452 1188.5497 1187.5424
2453 1188.5677 1187.5604
2454 1189.5476 1188.5403
2455 1189.5520 1188.5447
2456 1189.5735 1188.5662
2458 1190.5570 1189.5497
2459 1191.5428 1190.5355
2460 596.2933 1190.5720
2461 596.8218 1191.6290
2462 597.3002 1192.5858
2463 1194.5658 1193.5585
2464 597.7924 1193.5702
2465 598.2872 1194.5598
2466 1196.5623 1195.5550
2467 1196.5691 1195.5618
2468 1197.5542 1196.5469
2469 1197.5730 1196.5657
2470 599.2988 1196.5830
2473 600.7838 1199.5530
2474 600.7839 1199.5532
2475 600.8618 1199.7090
2476 601.2977 1200.5808
2477 601.3083 1200.6020
2478 601.7901 1201.5656
2481 603.7932 1205.5718
2484 604.8183 1207.6220
2485 604.8210 1207.6274
2486 604.8229 1207.6312
2491 607.7875 1213.5604
2492 607.7878 1213.5610
2494 608.7810 1215.5474
2495 608.8233 1215.6320
2496 609.2950 1216.5754
2497 609.7882 1217.5618
2498 609.8108 1217.6070
2500 610.7876 1219.5606
2502 611.8296 1221.6446
2503 611.8302 1221.6458
2504 611.8353 1221.6560
2505 613.7625 1225.5104
2506 615.7850 1229.5554
2507 615.7858 1229.5570
2508 617.2927 1232.5708
2510 618.8152 1235.6158
2511 620.3233 1238.6320
2512 620.7990 1239.5834
2513 620.8140 1239.6134
2515 621.3262 1240.6378
2516 621.8023 1241.5900
2517 623.3359 1244.6572
2518 625.3317 1248.6488
2519 625.3330 1248.6514
2523 628.3085 1254.6024
2527 629.8006 1257.5866
2528 629.8011 1257.5876
2529 629.8095 1257.6044
2533 631.3197 1260.6248
2534 631.3347 1260.6548
2535 631.3353 1260.6560
2538 635.8306 1269.6466
2540 636.2977 1270.5808
2542 636.3048 1270.5950
2543 636.3103 1270.6060
2544 636.3237 1270.6328
2546 637.8251 1273.6356
2547 638.3229 1274.6312
2548 638.7946 1275.5746
2549 638.8352 1275.6558
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