MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2183 1122.5317 1121.5244
2184 1122.5326 1121.5253
2185 1122.5459 1121.5386
2186 1123.5295 1122.5222
2187 1123.5332 1122.5259
2188 1123.5513 1122.5440
2189 1124.5159 1123.5086
2190 1124.5289 1123.5216
2191 1124.5370 1123.5297
2192 1124.5428 1123.5355
2193 562.7899 1123.5652
2195 375.8401 1124.4985
2196 1125.5139 1124.5066
2197 1125.5291 1124.5218
2198 563.2690 1124.5234
2199 1125.5358 1124.5285
2200 1125.5365 1124.5292
2201 1125.5447 1124.5374
2203 563.7592 1125.5038
2204 563.7621 1125.5096
2205 1126.5256 1125.5183
2206 1126.5261 1125.5188
2207 1127.5243 1126.5170
2208 1127.5399 1126.5326
2209 1127.5496 1126.5423
2211 1128.5328 1127.5255
2212 565.2791 1128.5436
2213 1130.5365 1129.5292
2214 1130.5421 1129.5348
2215 1130.5546 1129.5473
2216 1131.5275 1130.5202
2217 1131.5396 1130.5323
2218 1132.5322 1131.5249
2219 1132.5419 1131.5346
2221 1132.5535 1131.5462
2222 1132.5547 1131.5474
2223 1133.5282 1132.5209
2224 1133.5327 1132.5254
2225 1133.5441 1132.5368
2226 1133.5557 1132.5484
2227 567.2845 1132.5544
2228 1133.5637 1132.5564
2230 1134.5477 1133.5404
2231 567.7812 1133.5478
2232 1135.5435 1134.5362
2233 1136.5299 1135.5226
2234 568.7720 1135.5294
2235 1136.5419 1135.5346
2236 1136.5559 1135.5486
2238 569.2628 1136.5110
2239 1137.5446 1136.5373
2240 1137.5571 1136.5498
2241 1138.5400 1137.5327
2243 1139.5333 1138.5260
2244 1139.5350 1138.5277
2246 1139.5476 1138.5403
2247 1139.5505 1138.5432
2248 1140.5288 1139.5215
2249 1140.5325 1139.5252
2250 1140.5422 1139.5349
2251 1140.5505 1139.5432
2252 1141.5288 1140.5215
2254 1141.5568 1140.5495
2255 1141.5591 1140.5518
2256 571.2982 1140.5818
2257 571.3077 1140.6008
2258 1142.5176 1141.5103
2259 1142.5194 1141.5121
2261 571.7641 1141.5136
2262 1142.5365 1141.5292
2263 1142.5394 1141.5321
2264 1142.5509 1141.5436
2265 381.8458 1142.5156
2266 1143.5286 1142.5213
2267 1143.5471 1142.5398
2268 1144.5353 1143.5280
2269 572.7969 1143.5792
2270 572.7976 1143.5806
2271 1144.5991 1143.5918
2272 1145.5397 1144.5324
2273 1145.5591 1144.5518
2275 1146.5287 1145.5214
2276 1146.5385 1145.5312
2277 1146.5427 1145.5354
2278 1146.5443 1145.5370
2279 1146.5447 1145.5374
2280 1146.5497 1145.5424
2281 1146.5662 1145.5589
2283 1147.5494 1146.5421
2284 1147.5565 1146.5492
2285 1148.5396 1147.5323
2286 1148.5531 1147.5458
2287 1148.5643 1147.5570
2290 1149.5411 1148.5338
2291 575.2814 1148.5482
2292 1149.5569 1148.5496
2293 575.2844 1148.5542
2294 1149.5667 1148.5594
2296 575.2872 1148.5598
2297 1150.5430 1149.5357
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