MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2056 1090.5186 1089.5113
2057 1090.5239 1089.5166
2058 1091.5189 1090.5116
2059 1091.5237 1090.5164
2060 1091.5291 1090.5218
2061 1091.5358 1090.5285
2062 1092.5568 1091.5495
2064 1093.5153 1092.5080
2065 1093.5161 1092.5088
2066 1093.5331 1092.5258
2067 1093.5355 1092.5282
2068 1094.5195 1093.5122
2070 1094.5328 1093.5255
2072 1095.5197 1094.5124
2074 1095.5248 1094.5175
2075 1095.5249 1094.5176
2076 548.7503 1095.4860
2077 1096.5143 1095.5070
2078 1096.5310 1095.5237
2080 549.0034 1095.9922
2081 1097.4940 1096.4867
2082 1097.5116 1096.5043
2083 1097.5289 1096.5216
2084 1097.5310 1096.5237
2085 1098.5229 1097.5156
2086 1098.5267 1097.5194
2087 1098.5358 1097.5285
2088 1098.5376 1097.5303
2089 1099.5320 1098.5247
2091 1100.5187 1099.5114
2092 1100.5316 1099.5243
2093 1100.5394 1099.5321
2094 1100.5439 1099.5366
2095 550.7849 1099.5552
2096 1101.5360 1100.5287
2099 1102.5278 1101.5205
2102 1103.5179 1102.5106
2103 1103.5269 1102.5196
2104 552.2844 1102.5542
2106 1104.5135 1103.5062
2107 1104.5260 1103.5187
2108 1104.5272 1103.5199
2109 1105.5111 1104.5038
2110 1105.5173 1104.5100
2111 1105.5210 1104.5137
2112 1105.5302 1104.5229
2118 1106.5232 1105.5159
2119 1106.5250 1105.5177
2120 1106.5264 1105.5191
2122 1107.5131 1106.5058
2123 1107.5259 1106.5186
2124 1107.5283 1106.5210
2125 1107.5325 1106.5252
2126 1107.5543 1106.5470
2127 554.7684 1107.5222
2128 1108.5375 1107.5302
2130 1109.5236 1108.5163
2132 1109.5344 1108.5271
2133 1110.5208 1109.5135
2134 1110.5354 1109.5281
2135 1110.5359 1109.5286
2136 1110.5372 1109.5299
2137 1111.5028 1110.4955
2138 1111.5204 1110.5131
2139 1111.5245 1110.5172
2140 1111.5345 1110.5272
2141 1111.5366 1110.5293
2142 1111.5374 1110.5301
2143 1112.5352 1111.5279
2144 1113.5360 1112.5287
2145 1113.5475 1112.5402
2147 557.2940 1112.5734
2148 557.2945 1112.5744
2149 557.2961 1112.5776
2150 557.7592 1113.5038
2152 557.7610 1113.5074
2153 1114.5186 1113.5113
2154 1114.5189 1113.5116
2155 1114.5254 1113.5181
2157 1115.5220 1114.5147
2158 1115.5276 1114.5203
2159 1116.5291 1115.5218
2160 1116.5337 1115.5264
2161 1116.5439 1115.5366
2162 1117.5227 1116.5154
2163 1117.5396 1116.5323
2164 1117.5527 1116.5454
2165 1118.5226 1117.5153
2166 1118.5439 1117.5366
2167 1118.5464 1117.5391
2168 1119.5317 1118.5244
2169 1119.5342 1118.5269
2172 1120.5405 1119.5332
2175 1121.5176 1120.5103
2176 1121.5186 1120.5113
2177 1121.5304 1120.5231
2178 1121.5333 1120.5260
2179 561.3244 1120.6342
2181 561.7591 1121.5036
2182 1122.5267 1121.5194
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