MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1819 1027.4839 1026.4766
1820 1027.4900 1026.4827
1821 1028.4775 1027.4702
1822 1028.4945 1027.4872
1823 1028.5044 1027.4971
1824 1028.5092 1027.5019
1825 1029.4696 1028.4623
1826 1029.4811 1028.4738
1827 1029.5043 1028.4970
1828 1030.4967 1029.4894
1829 1030.4982 1029.4909
1830 1030.4988 1029.4915
1832 1031.4930 1030.4857
1833 1031.4983 1030.4910
1834 1032.4703 1031.4630
1835 516.7659 1031.5172
1836 1033.4889 1032.4816
1837 1033.4976 1032.4903
1838 1033.5012 1032.4939
1839 1033.5077 1032.5004
1840 1033.5165 1032.5092
1841 1034.4989 1033.4916
1842 1034.4990 1033.4917
1843 1034.5096 1033.5023
1845 518.2070 1034.3994
1846 345.8071 1034.3995
1847 1035.4863 1034.4790
1848 1035.5089 1034.5016
1849 1036.4877 1035.4804
1850 1036.4976 1035.4903
1851 1036.4979 1035.4906
1852 1036.5001 1035.4928
1854 1037.5016 1036.4943
1855 1037.5042 1036.4969
1857 1038.5024 1037.4951
1858 1038.5050 1037.4977
1860 1039.4822 1038.4749
1861 1039.4976 1038.4903
1862 520.2563 1038.4980
1863 1040.4780 1039.4707
1864 1040.5005 1039.4932
1865 1040.5011 1039.4938
1866 1040.5018 1039.4945
1867 1041.4879 1040.4806
1868 1041.4900 1040.4827
1869 1042.5088 1041.5015
1870 1042.5127 1041.5054
1871 521.7628 1041.5110
1873 521.7635 1041.5124
1874 1043.5023 1042.4950
1875 1043.5060 1042.4987
1876 1043.5110 1042.5037
1877 522.7339 1043.4532
1878 1044.4954 1043.4881
1879 1044.5015 1043.4942
1880 522.7817 1043.5488
1881 1046.4855 1045.4782
1883 1047.4841 1046.4768
1884 1047.5035 1046.4962
1885 1047.5193 1046.5120
1886 524.2725 1046.5304
1887 1048.4966 1047.4893
1888 1048.5085 1047.5012
1892 1048.5468 1047.5395
1893 524.7777 1047.5408
1894 524.7779 1047.5412
1896 1049.4891 1048.4818
1897 1049.5004 1048.4931
1898 1049.5009 1048.4936
1899 1049.5012 1048.4939
1901 525.2874 1048.5602
1902 525.2929 1048.5712
1903 525.6895 1049.3644
1904 1050.4990 1049.4917
1905 1050.5002 1049.4929
1906 1050.5018 1049.4945
1907 351.1312 1050.3718
1908 1051.5050 1050.4977
1909 1052.4978 1051.4905
1910 1052.5023 1051.4950
1912 1053.5034 1052.4961
1913 1053.5050 1052.4977
1914 1053.5187 1052.5114
1915 527.2912 1052.5678
1916 527.2913 1052.5680
1917 1054.0015 1052.9942
1920 1054.4971 1053.4898
1921 1054.5065 1053.4992
1922 1054.5072 1053.4999
1923 1054.5104 1053.5031
1924 1054.5173 1053.5100
1925 1055.4988 1054.4915
1926 1055.5070 1054.4997
1927 1055.5071 1054.4998
1928 1055.5221 1054.5148
1929 1056.5100 1055.5027
1933 1057.5231 1056.5158
1935 529.7510 1057.4874
1936 529.7510 1057.4874
1937 1058.5045 1057.4972
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