MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1701 992.4860 991.4787
1702 496.7634 991.5122
1703 496.7635 991.5124
1704 496.7636 991.5126
1705 496.7651 991.5156
1706 992.9830 991.9757
1707 993.4633 992.4560
1708 993.4867 992.4794
1709 993.4941 992.4868
1710 995.4722 994.4649
1711 995.4769 994.4696
1712 995.4870 994.4797
1714 996.4827 995.4754
1717 499.2335 996.4524
1719 997.4608 996.4535
1720 997.4677 996.4604
1721 997.4753 996.4680
1722 997.5225 996.5152
1723 998.4676 997.4603
1724 998.4744 997.4671
1725 998.4756 997.4683
1726 999.4587 998.4514
1728 500.2429 998.4712
1729 999.4813 998.4740
1730 999.4817 998.4744
1731 999.4875 998.4802
1732 1000.4963 999.4890
1733 1001.4733 1000.4660
1734 1001.4743 1000.4670
1735 1001.4820 1000.4747
1736 1001.4834 1000.4761
1737 1001.4837 1000.4764
1738 1002.4802 1001.4729
1739 1002.4805 1001.4732
1740 1002.4821 1001.4748
1741 1002.4854 1001.4781
1742 1003.4697 1002.4624
1743 1004.4884 1003.4811
1744 1004.8290 1003.8217
1745 1005.4753 1004.4680
1746 1005.4831 1004.4758
1747 1005.4868 1004.4795
1748 1006.4744 1005.4671
1749 1006.4802 1005.4729
1750 1006.4811 1005.4738
1752 1006.4937 1005.4864
1755 1007.4718 1006.4645
1756 1007.4800 1006.4727
1757 504.2507 1006.4868
1758 504.2628 1006.5110
1759 1007.5184 1006.5111
1760 504.2640 1006.5134
1761 504.7267 1007.4388
1763 504.7471 1007.4796
1764 1008.4882 1007.4809
1765 1008.4994 1007.4921
1766 1008.7154 1007.7081
1767 1009.4772 1008.4699
1768 1009.4850 1008.4777
1769 1010.4867 1009.4794
1770 1011.4769 1010.4696
1771 1011.4782 1010.4709
1772 1011.4940 1010.4867
1773 506.7354 1011.4562
1774 1012.4667 1011.4594
1775 1013.4818 1012.4745
1776 1013.4894 1012.4821
1777 1013.4922 1012.4849
1778 1015.4838 1014.4765
1779 1015.4857 1014.4784
1780 1015.4863 1014.4790
1783 1017.4913 1016.4840
1784 510.2242 1018.4338
1785 1019.4412 1018.4339
1786 1019.4854 1018.4781
1787 1019.4968 1018.4895
1788 1019.4972 1018.4899
1789 1020.4758 1019.4685
1790 1020.4810 1019.4737
1791 1020.4886 1019.4813
1792 1020.4962 1019.4889
1793 1020.5171 1019.5098
1794 1021.4774 1020.4701
1795 1021.4863 1020.4790
1800 1022.4801 1021.4728
1802 1022.5309 1021.5236
1803 512.2471 1022.4796
1805 1023.4898 1022.4825
1807 512.2605 1022.5064
1808 1023.5156 1022.5083
1809 512.2838 1022.5530
1810 1024.4819 1023.4746
1811 1024.4996 1023.4923
1812 1025.4891 1024.4818
1813 1025.4976 1024.4903
1814 1025.5015 1024.4942
1815 1025.5034 1024.4961
1816 513.2947 1024.5748
1817 1026.4897 1025.4824
1818 1026.5013 1025.4940
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