MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1586 952.4650 951.4577
1587 952.4758 951.4685
1588 952.5221 951.5148
1590 953.4585 952.4512
1591 953.4594 952.4521
1592 954.4626 953.4553
1594 955.4962 954.4889
1596 956.4563 955.4490
1597 956.4587 955.4514
1598 478.7363 955.4580
1599 478.7629 955.5112
1600 957.4614 956.4541
1601 958.4749 957.4676
1602 959.4623 958.4550
1603 959.4657 958.4584
1604 959.4695 958.4622
1605 959.4749 958.4676
1606 960.4565 959.4492
1607 960.4662 959.4589
1608 960.4678 959.4605
1609 960.4798 959.4725
1610 480.7676 959.5206
1611 961.4565 960.4492
1612 961.4686 960.4613
1613 962.4679 961.4606
1614 963.4667 962.4594
1615 964.4649 963.4576
1616 964.4763 963.4690
1617 965.4643 964.4570
1618 965.4693 964.4620
1619 965.4775 964.4702
1620 483.2580 964.5014
1622 483.7363 965.4580
1623 966.4656 965.4583
1624 966.4667 965.4594
1625 483.7747 965.5348
1626 484.2140 966.4134
1627 484.2463 966.4780
1629 484.7351 967.4556
1630 968.4655 967.4582
1632 968.4761 967.4688
1634 484.7443 967.4740
1635 969.4595 968.4522
1636 969.4633 968.4560
1637 969.4696 968.4623
1638 970.4716 969.4643
1640 971.4625 970.4552
1641 971.4749 970.4676
1642 973.4581 972.4508
1643 973.4622 972.4549
1644 973.4636 972.4563
1645 973.4698 972.4625
1646 973.4719 972.4646
1647 487.2473 972.4800
1648 974.4594 973.4521
1649 974.4605 973.4532
1650 975.4620 974.4547
1651 975.4836 974.4763
1653 976.4755 975.4682
1654 488.7654 975.5162
1655 977.4581 976.4508
1657 490.2281 978.4416
1658 979.4698 978.4625
1659 979.4774 978.4701
1660 980.4678 979.4605
1662 980.4830 979.4757
1663 981.4661 980.4588
1664 491.2371 980.4596
1665 981.4819 980.4746
1666 982.4572 981.4499
1667 982.4728 981.4655
1668 982.4779 981.4706
1669 982.4786 981.4713
1670 982.4791 981.4718
1671 982.4835 981.4762
1672 983.4597 982.4524
1673 983.4757 982.4684
1674 983.4866 982.4793
1677 984.4952 983.4879
1678 985.4650 984.4577
1679 985.4755 984.4682
1680 985.4884 984.4811
1681 493.2521 984.4896
1682 986.4711 985.4638
1683 986.4785 985.4712
1684 986.4796 985.4723
1685 986.4824 985.4751
1686 987.4602 986.4529
1687 987.4768 986.4695
1688 987.4826 986.4753
1690 989.4982 988.4909
1691 990.4750 989.4677
1692 990.4755 989.4682
1693 990.9767 989.9694
1695 991.5261 990.5188
1696 496.2673 990.5200
1697 992.4722 991.4649
1698 992.4726 991.4653
1699 992.4785 991.4712
1700 496.7451 991.4756
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