MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1460 911.4464 910.4391
1463 456.2354 910.4562
1464 912.4364 911.4291
1465 913.4302 912.4229
1466 913.4431 912.4358
1467 913.4549 912.4476
1469 915.4352 914.4279
1470 916.4345 915.4272
1471 916.4578 915.4505
1472 917.4373 916.4300
1473 917.4473 916.4400
1474 459.7441 917.4736
1475 919.4417 918.4344
1476 919.4426 918.4353
1478 920.4398 919.4325
1479 920.4494 919.4421
1480 921.4368 920.4295
1481 921.4382 920.4309
1482 921.4386 920.4313
1483 921.9831 920.9758
1484 922.4431 921.4358
1485 461.7549 921.4952
1486 923.4429 922.4356
1487 923.4485 922.4412
1488 923.4555 922.4482
1489 924.4073 923.4000
1490 462.7075 923.4004
1491 924.4586 923.4513
1492 924.4738 923.4665
1494 926.4374 925.4301
1495 926.4385 925.4312
1496 926.4402 925.4329
1497 926.4445 925.4372
1498 926.4511 925.4438
1499 926.4557 925.4484
1500 927.4410 926.4337
1501 309.8229 926.4469
1502 309.8229 926.4469
1503 464.2315 926.4484
1505 928.4387 927.4314
1507 464.7415 927.4684
1508 929.1556 928.1483
1509 929.4656 928.4583
1510 930.4349 929.4276
1511 930.4495 929.4422
1513 931.4409 930.4336
1514 931.4429 930.4356
1515 931.4578 930.4505
1516 931.4598 930.4525
1517 931.9885 930.9812
1518 932.4513 931.4440
1519 932.4527 931.4454
1520 932.4532 931.4459
1521 932.4540 931.4467
1522 932.4575 931.4502
1523 466.7325 931.4504
1524 932.4583 931.4510
1525 933.4435 932.4362
1526 934.4370 933.4297
1527 467.7423 933.4700
1528 468.2186 934.4226
1529 935.4501 934.4428
1530 935.4572 934.4499
1532 936.4543 935.4470
1533 937.4632 936.4559
1536 938.4819 937.4746
1537 939.4174 938.4101
1538 939.4447 938.4374
1539 939.4522 938.4449
1540 939.4604 938.4531
1543 940.4492 939.4419
1544 940.4507 939.4434
1546 940.4756 939.4683
1549 941.4440 940.4367
1550 941.4564 940.4491
1551 941.4691 940.4618
1552 942.4511 941.4438
1553 942.4587 941.4514
1554 942.4615 941.4542
1555 942.4636 941.4563
1556 942.4678 941.4605
1557 942.4726 941.4653
1558 943.4421 942.4348
1559 472.2271 942.4396
1560 943.4645 942.4572
1561 944.4412 943.4339
1563 946.4537 945.4464
1564 946.4556 945.4483
1565 473.9752 945.9358
1566 947.4442 946.4369
1567 947.4521 946.4448
1568 947.4619 946.4546
1569 947.4625 946.4552
1571 947.4910 946.4837
1572 947.4928 946.4855
1574 948.4467 947.4394
1576 949.4452 948.4379
1579 475.2598 948.5050
1583 475.7404 949.4662
1585 476.2164 950.4182
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