MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1245_Other_P1_B11.mgf
Database : S-cerevisiae-S288C 20191206 (6,886 sequences; 3,133,934 residues)
Timestamp : 24 Jun 2025 at 21:21:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,722

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 4645)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1176 384.2066 766.3986 766.3861 16.4 0 8 0.58 +1Score > 20 indicates identity
Score > 18 indicates homology		 QLNYTK + Deamidated (NQ)
1856 519.7475 1037.4804 1037.4778 2.58 0 8 0.2 +1Score > 21 indicates identity
Score > 14 indicates homology		 IGETDYNAR
1337 858.4199 857.4126 857.4171 -5.18 0 8 0.52 +1Score > 18 indicates identity YLSNIFT + Deamidated (NQ)
2253 571.2793 1140.5440 1140.5485 -3.90 1 8 0.91 +1Score > 20 indicates identity GIAEVNMKYT + Oxidation (M)
3301 926.4230 1850.8314 1850.8396 -4.43 1 8 0.94 +1Score > 20 indicates identity QGLEGNSYKFLFGDMK + 2 Deamidated (NQ); Oxidation (M)
3203 888.4402 1774.8658 1774.8849 -10.8 0 8 0.59 +1Score > 22 indicates identity
Score > 18 indicates homology		 LSSYPTPNLLNTADNR
1781 508.2668 1014.5190 1014.5346 -15.3 1 8 0.37 +1Score > 21 indicates identity
Score > 16 indicates homology		 KDIGDDKPK
2174 560.7802 1119.5458 1119.5672 -19.1 1 8 0.2 +1Score > 22 indicates identity
Score > 13 indicates homology		 DIINKHNHK + 2 Deamidated (NQ)
3906 1108.9802 2215.9458 2215.9552 -4.23 0 8 0.52 +1Score > 17 indicates identity APMGANAAVDNNAAGGGEFNDPR + Deamidated (NQ)
2577 651.8715 1301.7284 1301.7455 -13.1 1 8 0.77 +1Score > 20 indicates identity
Score > 19 indicates homology		 SLDLYLAPRVR
1995 537.7862 1073.5578 1073.5604 -2.40 0 8 0.25 +1Score > 23 indicates identity
Score > 14 indicates homology		 VAEEQLSGIK + Deamidated (NQ)
4115 806.1042 2415.2908 2415.2686 9.20 1 8 0.72 +1Score > 19 indicates identity AIFPNTVDGDLLKLVHLSNGYK + 2 Deamidated (NQ)
1754 503.7704 1005.5262 1005.5165 9.73 1 8 1 +1Score > 23 indicates identity
Score > 20 indicates homology		 MKAGDDILK + Oxidation (M)
1038 655.3784 654.3711 654.3701 1.63 0 8 0.25 +1Score > 14 indicates identity TAAPAPK
2790 745.8610 1489.7074 1489.6783 19.5 0 8 0.26 +1Score > 22 indicates identity
Score > 14 indicates homology		 AVEALNDSELNGEK + 2 Deamidated (NQ)
2591 660.3345 1318.6544 1318.6703 -12.0 1 7 1 +1Score > 23 indicates identity
Score > 20 indicates homology		 RSNMFVPLPNK + Deamidated (NQ); Oxidation (M)
2791 745.8613 1489.7080 1489.6783 19.9 0 7 1 +1Score > 22 indicates identity
Score > 20 indicates homology		 AVEALNDSELNGEK + 2 Deamidated (NQ)
1449 906.4333 905.4260 905.4277 -1.80 0 7 1 +1Score > 23 indicates identity
Score > 20 indicates homology		 AVPSSSACK
2539 635.8484 1269.6822 1269.6573 19.6 0 7 0.2 +1Score > 20 indicates identity
Score > 13 indicates homology		 VPHMCALSLVK + Oxidation (M)
962 555.3148 554.3075 554.3176 -18.3 0 7 0.32 +1Score > 15 indicates identity ITGHK
2131 555.2666 1108.5186 1108.5301 -10.4 0 7 0.65 +1Score > 22 indicates identity
Score > 18 indicates homology		 LWNSQNGYK
1211 397.6881 793.3616 793.3640 -2.94 0 7 0.51 +1Score > 21 indicates identity
Score > 17 indicates homology		 MAANSVGK + Deamidated (NQ); Oxidation (M)
2229 567.2896 1132.5646 1132.5798 -13.4 1 7 0.6 +1Score > 22 indicates identity
Score > 17 indicates homology		 LDPKTAETMK
2904 521.6173 1561.8301 1561.8028 17.5 1 7 0.56 +1Score > 22 indicates identity
Score > 17 indicates homology		 GILNYYKLATNYK + 2 Deamidated (NQ)
1304 842.4397 841.4324 841.4341 -1.97 1 7 0.61 +1Score > 17 indicates identity CLHRTR
1306 421.7601 841.5056 841.5021 4.17 0 7 0.8 +1Score > 19 indicates identity GNVIILGR + Deamidated (NQ)
2045 544.2868 1086.5590 1086.5669 -7.24 1 7 0.55 +1Score > 22 indicates identity
Score > 17 indicates homology		 ERQVELEGK
1307 421.7604 841.5062 841.5021 4.89 0 7 0.81 +1Score > 19 indicates identity GNVIILGR + Deamidated (NQ)
2884 774.3687 1546.7228 1546.7184 2.86 0 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 LNQPSNINMEEIK + 2 Deamidated (NQ); Oxidation (M)
2041 544.2844 1086.5542 1086.5709 -15.4 0 7 0.45 +1Score > 22 indicates identity
Score > 16 indicates homology		 EHTIIAEFK
1341 430.2304 858.4462 858.4447 1.84 0 7 0.33 +1Score > 22 indicates identity
Score > 15 indicates homology		 QNGIGIQK + 2 Deamidated (NQ)
1582 475.7389 949.4632 949.4651 -1.95 0 7 1 +1Score > 22 indicates identity
Score > 19 indicates homology		 VASSAVMNR + Oxidation (M)
2071 548.2595 1094.5044 1094.5026 1.67 0 7 1 +1Score > 22 indicates identity
Score > 19 indicates homology		 GSTANMSLGGGK + Oxidation (M)
2715 723.8684 1445.7222 1445.7085 9.49 0 7 1 +1Score > 22 indicates identity
Score > 19 indicates homology		 SHGAVLPSYCSLR
1385 878.4504 877.4431 877.4579 -16.8 0 7 0.74 +1Score > 24 indicates identity
Score > 18 indicates homology		 TLGSIEMK
1512 465.7313 929.4480 929.4389 9.87 1 7 0.88 +1Score > 19 indicates identity QHMQKNK + Deamidated (NQ); Oxidation (M)
2599 664.8300 1327.6454 1327.6408 3.51 0 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 NSATILNYFER + Deamidated (NQ)
2713 723.8663 1445.7180 1445.7085 6.59 0 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 SHGAVLPSYCSLR
1422 898.3901 897.3828 897.3716 12.5 0 7 0.69 +1Score > 17 indicates identity ADSGYEEK
2514 414.5516 1240.6330 1240.6339 -0.78 0 7 0.25 +1Score > 23 indicates identity
Score > 13 indicates homology		 YVLYGVSTPNK + Deamidated (NQ)
2699 479.2332 1434.6778 1434.6860 -5.74 1 7 0.36 +1Score > 22 indicates identity
Score > 15 indicates homology		 WVMGLSNGQMRR + Deamidated (NQ)
1050 667.3545 666.3472 666.3561 -13.4 0 7 0.6 +1Score > 17 indicates identity LQNHR
2536 633.8248 1265.6350 1265.6140 16.7 0 7 0.33 +1Score > 22 indicates identity
Score > 14 indicates homology		 VESGLFSVDGEK
1593 477.7492 953.4838 953.4971 -13.9 1 7 0.91 +1Score > 20 indicates identity
Score > 19 indicates homology		 KSGWVSYK
1796 511.2723 1020.5300 1020.5200 9.87 1 6 0.32 +1Score > 23 indicates identity
Score > 14 indicates homology		 LTSGSGSERK
2100 552.2568 1102.4990 1102.5142 -13.7 0 6 0.99 +1Score > 19 indicates identity EGGETQEKPK + Deamidated (NQ)
3994 1152.0634 2302.1122 2302.1414 -12.7 1 6 0.26 +1Score > 22 indicates identity
Score > 13 indicates homology		 ISQSGQSQHPTRQQEPLPER
3175 875.9624 1749.9102 1749.9149 -2.63 1 6 0.29 +1Score > 22 indicates identity
Score > 13 indicates homology		 IRTYLDLLLDDDASK
3051 552.6063 1654.7971 1654.7951 1.18 1 6 1 +1Score > 22 indicates identity
Score > 19 indicates homology		 GLFKFSDVETQDGGR
2442 592.7629 1183.5112 1183.5292 -15.1 0 6 0.95 +1Score > 19 indicates identity QSQFNLMSGR + Deamidated (NQ); Oxidation (M)
2530 630.3008 1258.5870 1258.5902 -2.48 1 6 1 +1Score > 20 indicates identity
Score > 19 indicates homology		 QNLRQADDGNK + Deamidated (NQ)
1138 371.7103 741.4060 741.4021 5.35 0 6 0.77 +1Score > 18 indicates identity QLQQPK + Deamidated (NQ)
2531 630.3038 1258.5930 1258.5902 2.29 1 6 0.43 +1Score > 20 indicates identity
Score > 15 indicates homology		 QNLRQADDGNK + Deamidated (NQ)
2745 487.5856 1459.7350 1459.7154 13.4 0 6 1 +1Score > 23 indicates identity
Score > 19 indicates homology		 INGLNSNNSIIGNK + 3 Deamidated (NQ)
2101 1103.5088 1102.5015 1102.5142 -11.5 0 6 1 +1Score > 19 indicates identity EGGETQEKPK + Deamidated (NQ)
1144 372.2072 742.3998 742.4086 -11.7 1 6 0.77 +1Score > 18 indicates identity KANPTGR
3180 877.4395 1752.8644 1752.8539 6.04 1 6 0.89 +1Score > 23 indicates identity
Score > 18 indicates homology		 FGMKELDNDILGVMR + Oxidation (M)
2151 557.7593 1113.5040 1113.5012 2.55 1 6 1.1 +1Score > 19 indicates identity YIDDGMQKK + Deamidated (NQ); Oxidation (M)
2578 652.3013 1302.5880 1302.6125 -18.8 0 6 0.55 +1Score > 20 indicates identity
Score > 16 indicates homology		 MQESAPPDITAK + Oxidation (M)
1097 705.3211 704.3138 704.3089 6.95 0 6 0.95 +1Score > 18 indicates identity GNSGVDR + Deamidated (NQ)
2210 564.3023 1126.5900 1126.5692 18.5 1 6 1.2 +1Score > 19 indicates identity YNVIKSMQK + Deamidated (NQ); Oxidation (M)
2370 583.2843 1164.5540 1164.5411 11.1 0 6 0.68 +1Score > 22 indicates identity
Score > 17 indicates homology		 QVNFNQETGK + Deamidated (NQ)
2614 669.3482 1336.6818 1336.6986 -12.6 0 6 0.3 +1Score > 23 indicates identity
Score > 13 indicates homology		 NSLSISNLAYVR + Deamidated (NQ)
1243 405.6857 809.3568 809.3668 -12.2 0 6 0.93 +1Score > 18 indicates identity NAAYDTR
2662 695.8731 1389.7316 1389.7252 4.65 1 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 IRELLNFNDEK
1003 308.6874 615.3602 615.3592 1.77 0 6 1 +1Score > 25 indicates identity
Score > 18 indicates homology		 AVIEGK
2032 542.7861 1083.5576 1083.5421 14.4 1 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 AVNGNPDRNK
1656 978.4752 977.4679 977.4600 8.13 1 6 0.47 +1Score > 22 indicates identity
Score > 15 indicates homology		 SLNKMNNR + 2 Deamidated (NQ)
1799 511.2805 1020.5464 1020.5492 -2.66 0 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 VTLFNTTPK + Deamidated (NQ)
4190 843.0338 2526.0796 2526.0881 -3.38 1 6 0.92 +1Score > 18 indicates identity ASDDKEEQNEQATLQDISNFDK + 2 Deamidated (NQ)
1676 492.7378 983.4610 983.4784 -17.7 0 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 NPSQRPQR + 2 Deamidated (NQ)
1363 435.2660 868.5174 868.5243 -7.84 1 6 0.47 +1Score > 15 indicates identity ISLPQRR
2604 665.3776 1328.7406 1328.7551 -10.9 0 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 NLLQLTSIGEIK + Deamidated (NQ)
1137 742.4133 741.4060 741.4021 5.31 0 6 0.88 +1Score > 18 indicates identity AAEGPVAK
1029 322.6724 643.3302 643.3289 2.04 0 6 0.87 +1Score > 18 indicates identity LGSPDR
1008 624.3359 623.3286 623.3279 1.19 0 6 0.97 +1Score > 20 indicates identity
Score > 18 indicates homology		 VSGSFK
3304 463.9710 1851.8549 1851.8275 14.8 0 6 1 +1Score > 21 indicates identity
Score > 18 indicates homology		 IVQSWYNDLDVNDDR + Deamidated (NQ)
1300 421.2268 840.4390 840.4341 5.87 0 6 1.2 +1Score > 19 indicates identity NATPVNPK + Deamidated (NQ)
2795 746.8992 1491.7838 1491.7681 10.5 1 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 SPDLINKSTFQSR
3709 701.3399 2100.9979 2101.0368 -18.5 1 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 STFREQIIYPDSIEQFK + Deamidated (NQ)
2853 765.8783 1529.7420 1529.7469 -3.19 0 6 0.64 +1Score > 22 indicates identity
Score > 16 indicates homology		 MIMNSTNTVVYIK + Deamidated (NQ); Oxidation (M)
1534 938.4230 937.4157 937.4327 -18.2 1 6 1.3 +1Score > 19 indicates identity GEMKSGWK + Oxidation (M)
4092 797.4355 2389.2847 2389.2839 0.33 0 6 1 +1Score > 18 indicates identity EQISSLIDSLLLNSTNLSSLLK + 2 Deamidated (NQ)
1344 431.2145 860.4144 860.3988 18.2 0 6 0.63 +1Score > 22 indicates identity
Score > 16 indicates homology		 QLQDNSR + Deamidated (NQ)
2758 734.8589 1467.7032 1467.6987 3.09 1 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 LASASMNKTATSNR + Deamidated (NQ); Oxidation (M)
1562 472.7387 943.4628 943.4723 -10.0 0 6 1.4 +1Score > 19 indicates identity LGEGQIDGR
1713 498.7424 995.4702 995.4712 -0.98 0 6 0.93 +1Score > 21 indicates identity
Score > 18 indicates homology		 LEFGNYPR + Deamidated (NQ)
1918 1054.4797 1053.4724 1053.4839 -10.9 1 6 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 TDNQYTRR + Deamidated (NQ)
3823 1076.0228 2150.0310 2150.0612 -14.0 1 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 RSPTAECTALGAAIAANMAFK
3829 718.0323 2151.0751 2151.0742 0.40 1 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 VAVNSLENMPSQRLPGEPGR + Deamidated (NQ)
1412 446.7350 891.4554 891.4596 -4.69 1 6 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 LGMRGQSK + Oxidation (M)
1115 363.2009 724.3872 724.3908 -4.91 0 5 1.2 +1Score > 19 indicates identity IGNFFK
1325 427.2306 852.4466 852.4527 -7.15 0 5 1.4 +1Score > 19 indicates identity FSIIMAR + Oxidation (M)
1218 398.2224 794.4302 794.4174 16.1 0 5 0.74 +1Score > 20 indicates identity
Score > 17 indicates homology		 VSTTYPK
3531 1011.4574 2020.9002 2020.9082 -3.91 0 5 1 +1Score > 20 indicates identity
Score > 18 indicates homology		 LNPDGTMAIDEETMVVDR + Oxidation (M)
2051 1088.5400 1087.5327 1087.5397 -6.40 0 5 0.62 +1Score > 22 indicates identity
Score > 16 indicates homology		 QINDLQIDK + 2 Deamidated (NQ)
2934 789.9188 1577.8230 1577.8089 8.94 0 5 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 YSLPPQTIQDLFR + Deamidated (NQ)
3848 722.3472 2164.0198 2163.9953 11.3 0 5 0.54 +1Score > 22 indicates identity
Score > 15 indicates homology		 NMTNSAASLATSPTSNQLNPK + 2 Deamidated (NQ); Oxidation (M)
1150 751.3749 750.3676 750.3548 17.1 0 5 0.76 +1Score > 22 indicates identity
Score > 17 indicates homology		 QPYESK
2804 750.8653 1499.7160 1499.7157 0.22 0 5 1 +1Score > 21 indicates identity
Score > 18 indicates homology		 YHSHNDTIFIPR + Deamidated (NQ)
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